Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 73 for “"LC3"”.
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
Atg8 and its eukaryotic orthologues LC3 and GARBARAP family proteins (referred here to Atg8 family proteins) play crucial roles in autophagy through their covalent ligation to lipids, typically phosphatidylethanolamine (PE), in a process known as lipidation. Lipidation of Atg8 family proteins …
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Discovering LC3-interacting region (LIR) motifs in hemorrhagic fever viruses: Implications for host autophagy and viral replication.
… factor of RVFV and we discovered four novel LC3-interacting region (LIR) motifs within NSs (NSs1-4), indicating that NSs interacts with LC3, the host key autophagy protein. Autophagy is a cellular process that can act anti-virally by enhancing immune responses and promoting viral degradation, …
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Admixture compatibility and freeze-thaw resistance of concrete prepared using commercially produced limestone calcined clay cements
… produced limestone calcined clay cements (LC3s) were evaluated during this study. Incompatibility was evaluated for commercially available high range water reducers (HRWRs) and air entraining agents (AEAs) by studying the impact on flow diameters and initial setting time of mortars. …
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Characterisation of a novel interaction partner for Atg16L1 and its role in regulating autophagy
… of nutrient deprivation. Membrane targeting of LC3, the major structural protein of the autophagosome, is essential for autophagy. LC3 is processed from its cytosolic form (LC3-I) to its lipidated form (LC3-II) in a series of ubiquitin-like conjugation events. Of particular importance is …
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Novel mechanisms of Atg16L1 recruitment in non-canonical autophagy
… is the recruitment, processing and lipidation of LC3 to autophagosomes. Recently it has become increasingly apparent that, through the unconventional use of some autophagy related proteins, LC3 can also become lipidated to distinct non-autophagosomal membranes of the endolysosomal system. This …
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Directed Evolution of Macrocyclic Peptides For Inhibition of Autophagy
… peptides targeting the autophagy protein LC3. The resulting peptides bound LC3A and LC3B—two essential components of the autophagosome maturation machinery—with mid-nanomolar affinities and disrupted protein-protein interactions (PPIs) between LC3 and its binding partners in vitro. …
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Investigation of selected autophagic proteins and the effect of tPS1-GFP expression.
… selected autophagy regulators (mTOR, raptor and LC3 proteins) and subsequently on the autophagy process. To investigate this, many different commercial antibodies were characterized against whole cell lysates from three different cell lines (NRK, MCF-7 and HEK293) using western blotting to obtain …
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Polar Body Membrane Breakdown Occurs Independent of Macroautophagy in C. Elegans
… disorders in mammals. To better understand if LC3-associated phagocytosis (LAP) drives cell corpse breakdown in phagolysosomes, we studied the clearance of the <em>C. elegans</em> polar body during embryonic development. ATG16L1 has separable roles in regulating LC3 recruitment during …
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The study of two transmembrane autophagy proteins and the autophagy receptor, p62
… can specifically associate with and sequester LC3-I in autophagy-impaired cells (ATG9 and ATG16 null cells) leading to formation of LC3-positive structures that can be misinterpreted as mature autophagosomes. Perturbations in the levels of p62 were seen to affect the formation of these …
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Development of low-clinker concrete: partial replacement of cement with calcined clay and limestone, based on selected African raw materials
… countries. An attractive option is to produce LC3 binders, which consist of ground limestone, calcined kaolinite clay, and cement. In this study, the suitability of kaolinite clays for use in LC3 binders from selected deposits in South Africa and Tanzania was assessed. Four samples of clays …
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Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
… tumours express high levels of autophagy marker LC3. Bioinformatics showed how MAP1LC3B (LC3B gene) can be highly expressed in tumours associated with poor disease outcome. Drug-treated OS cell lines showed a remarkable increase in LC3 puncta (autophagy marker) detected by Immunofluorescence. …
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Visualization and quantification of autophagy in primary gastrointestinal epithelia cell cultures.
… from human intestinal biopsy material. Atg8/LC3 is the major structural protein of the autophagosome. Images of GFP-Atg8/LC3 puncta generated in response to starvation, or NOD2 signalling, were taken by fluorescence microscopy, and pixel densities were rendered to generate digital datasets …
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Cationic amphiphilic drug-induced autophagosome accumulation is due to autophagosome sequestration within vimentin intermediate filament networks resulting in prolonged autophagosome half-life
… accumulation.</p> <p>Measurement of LC3-II conversion in the presence of lysosomal inhibitors</p> <p>bafilomycin A1 and NH4Cl, degradation of long-lived proteins, and</p> <p>colocalization of GFP-LC3 and LAMP1 indicated an increase in</p> <p>autophagosome synthesis without …
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Μελέτη του ρόλου των εξωσωματίων των μεσεγχυματικών στρωματικών κυττάρων στην οξεία ηπατική ανεπάρκεια
… ειδικών μοριακών δεικτών, όπως η μετατροπή της LC3-I σε LC3-II (δείκτης σχηματισμού αυτοφαγοσωμάτων) και τα επίπεδα της πρωτεΐνης p62 (sequestosome 1), η οποία συσσωρεύεται σε περιπτώσεις παρεμποδισμένης αυτοφαγίας, αξιολογήθηκε η λειτουργικότητα του μηχανισμού υπό συνθήκες ηπατικής βλάβης. Τα …
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Autophagy During and Following Dynamic Exercise in Young Adults: Influence of Exercise Intensity
… condition. A significant increase (p≤0.05) in LC3-II and LC3-II/I protein expression was observed immediately post high-, but not low- or moderate-intensity exercise. Additionally, elevations in LC3-II immediately following high intensity exercise in a hot environment were significantly greater …
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Interactions between Human Two-pore Channels and Nonaspanin Proteins
… Indeed, my results showed that TM9SF1 increases LC3-II/LC3-I ratio in TPCs knockdown groups significantly compared with groups without TPCs knockdown, which indicates TM9SF1-induced autophagosome formation is dependent on TPCs knockdown. Therefore, it is of great interest to pursue to discover …
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Mitochondrial response to axonal injury
… (mitoDsRed) as well as fluorescently tagged LC3 (GFP-LC3), an autophagosomal marker, was injected into primary motor cortex, to label the corticospinal tract (CST), of adult rats. The axons of the CST were then injured by dorsal column lesion at C4-C5. We found that mitochondria in injured …
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Functional characterisation of ANKH mutants in the pathology of CPPDD and CMD
… and the interactions with autophagy marker LC3 was investigated. Next we determined extracellular inorganic pyrophosphate (ePPi), mineralization, ENPP1 activity, expression of ENPP1, TNAP and PIT-1 to characterise mutant biochemical effects in the pathogenesis of CPPDD and CMD. Our data …
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Records of Great Basin precipitation during MIS 11 from two Lehman Cave stalagmites
… were measured in two coeval stalagmites, LC3 and BT1, from the Lehman Caves, Nevada. BT1 spans 388 to 384 ka. The LC3 record is split into two parts due to a hiatus observed through fluorescent imaging. The section prior to the hiatus spans 411 ka to 402 ka. The post-hiatus section of the …
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Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target
… of microtubule-associated protein light chain 3 (LC3) by western blot, confocal microscopy, transmission electron microscopy, and flow cytometry. IL-8 levels were measured by ELISA. To induce autophagy, HL-60 cells, SAECs, and A549 cells were treated with IL-5, IL-1β, and TNF-α. To inhibit …
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