Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"LC-ESI-MS/MS"”.
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LC-ESI-MS/MS quantification of hair cortisol, cortisone and DHEA in combined oral contraceptive users
… many COC adverse effects coincide with symptoms of stress-related illnesses, including insulin resistance and hypertension, it is believed that these adverse effects are a result of chronic biological stress from long-term COC use and that COC users are at greater risk of developing …
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Investigation of protein induction in vascular-targeted strategies.
The aims of the study reported in this thesis were to develop and utilise mass spectrometry imaging techniques (MALDI-MSI), in combination with conventional proteomic methodologies, to investigate protein induction in vascular-targeted strategies. Proteins thought to be involved in tumourigenesis …
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Computational methods in palaeoproteomics: making the most of mass spectrometry data
This thesis addresses the computational challenges that hinder progress in zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI MS/MS) based palaeoproteomics on ceramics, advocating for the development of open-source, …
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Structural Studies on the Estrogen Receptor Ligand Binding Domain Using Electrospray Ionization Mass Spectrometry
A protocol using LC/ESI-MS and LC/ESI-MS/MS was developed and used successfully to identify the site covalent attachment of the photoaffinity label hexestrol diazirine. The application of tandem MS/MS sequencing permitted the identification of several residues near the C-terminus of the protein.
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Towards understanding the fate of perfluoroalkyl compounds (PFCs) within urban environments: implications for human exposure.
… about human exposure. The purpose of this thesis is to investigate the presence of perfluoroalkyl compounds (PFCs) in UK indoor and outdoor microenvironments and the impact this may have on human exposure. Both PFOS and PFOA were chosen for analysis (via LC-ESI-MS/MS) because of their highly …
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Investigating the sources and magnitude of human exposure to halogenated organic pollutants using advanced methods for environmental analysis.
Analytical methods based on LC-ESI-MS/MS or LC-APPI-MS/MS were developed and/or validated for the separation and determination of BDE-209 and TBBP-A, in addition to HBCD diastereomers, enantiomers and degradation products in a wide range of samples including air, dust, diet, simulated GIT fluid, …
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The Group IVA Cytosolic Phospholipase A2/C1P Interaction and Its Role in Eicosanoid Synthesis and Inflammation
… is crucial for production of eicosanoid synthesis in inflammation. Inflammation is a critical component of many disease states including anaphylaxis, cancer, cardiovascular disease, rheumatoid arthritis, diabetes and asthma. Eicosanoids are well established mediators of inflammation, and the …
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Mass Spectrometric Characterization of the MCF7 Cancer Cell Line: Proteome Profile and Cancer Biomarkers
… technologies with mass spectrometry detection (MS) have the potential to affect the entire spectrum of cancer research by identifying these biomarkers. Simultaneously, microfabricated devices have evolved into ideal analysis platforms for minute amounts of sample, with promising applications for …
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Characterization of ceramide synthases (Cers) in mammalian cells
This thesis describes the characterization of ceramide (Cer) biosynthesis by mammalian cells. The possibility that Cer undergo developmental changes was explored using mouse embryonic stem cells versus embryoid bodies by analysis of the Cer subspecies by liquid chromatography, electrospray …
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The genetic and biochemical analysis of Drosophila Wwox protein function.
… subjected to targeted ‘loss of function’ mutagenesis. The resulting mutants were found to be viable when homozygous with no obvious defects in the adult fly. As Wwox mutant flies were found to exhibit an increased sensitivity to ionising radiation (IR), a number of Wwox proteins specifically …
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The effect of eicosapentaenoic acid on brain and platelet produced bioactive lipid mediators. The effect of eicosapentaenoic acid, docosapentaenoic acid and other polyunsaturated fatty acids on the eicosanoids and endocannabinoids produced by rat brain and human platelets using electrospray ionisation tandem mass spectrometry-based analysis.
… and pathological processes in many bodily systems. The purpose of this study was to examine the production of eicosanoids, hydroxy fatty acids and fatty acid ethanolamides in young and aged rat brain following EPA or DPA enriched diets. The effects of specific PUFA on human platelet eicosanoid …
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Studies on Bioactive Lipid Mediators Involved in Brain Function and Neurodegenerative Disorders. The effect of ¿-3PUFA supplementation and lithium treatment on rat brain sphingomyelin species and endocannabinoids formation; changes in oxysterol profiles in blood of ALS patients and animal models of ALS.
… production of such lipid mediators. The aims of this study were to evaluate the effects of n-3 EPA and n-3 DPA supplementation on RBC membranes and in this way assess dietary compliance and to investigate brain sphingomyelin species of adult and aged rats supplemented with n-3 EPA and n-3 …
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KINETICS AND PROTEOLYTIC SIGNATURES OF HISTATIN 5 DEGRADATION IN SALIVA AND GINGIVAL CREVICULAR FLUID IN PERIODONTAL HEALTH AND GINGIVITIS
… ionization tandem mass spectrometry (LC-ESI-MS/MS)) and degradation kinetics (Cationic polyacrylamide gel electrophoresis (PAGE) with band degradation quantification), can provide insights into periodontal disease activity in saliva and gingival crevicular fluid (GCF). Four objectives …
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Functionality of Azadirachta indica A. Juss (neem) in beverages
… has rekindled interest in age old medicinal systems such as Ayurveda and Unani. The various trees, herbs and shrubs used in these ancient practices are being incorporated today into foods and beverages to meet the health expectations of consumers from their diet. There is an intense curiosity …
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Toxicological evaluation of azole fungicides in agriculture and food chemistry
… CYP19 wie auch humanes CYP51 sollten durch ein solches Azol nicht inhibiert werden. Ein ideales Azol-Zytostatikum sollte eine starke inhibitorische Potenz gegenüber humanem CYP19 aufweisen, hingegen sollten humanes und Pilz-CYP51 nicht inhibiert werden. Ziel dieser Arbeit war es nun festzustellen: …
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An examination of the bioactive lipids involved in skin cell inflammation and in response to ultraviolet radiation. Effect of n-3 polyunsaturated fatty acid supplementation on red blood cell and human dermal fatty acid and production of eicosanoids by HaCaT keratinocytes and 46BR.1N fibroblasts following exposure to UVR.
… as photoprotective agents, their exact mechanisms of action is not completely understood. The aim of the work is to determine the effect of UVR and the n-3 PUFA eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA) on human skin cells (in vitro study), specifically on: cell viability, …
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Production, characterization and evaluation of anti-diabetes peptides from proteins of improved common bean (Phaseolus vulgaris L.) cultivars using bioinformatic tools, enzymatic systems, and in vitro and in vivo models
… attributed to bioactive compounds; including resistant starch and oligosaccharides that contribute to dietary fiber; phenolic compounds and bioactive peptides released from proteins during enzymatic digestion. Biological potential of bean peptides has been related to antihypertensive, …
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Applications of LC-MS/MS in forensic toxicology for the analysis of drugs and their metabolites
This thesis studied opioids and alcohol in forensic toxicology by LC-MS/MS, which avoids time-consuming procedures involving hydrolysis, extraction and derivatisation. Initially, a method was validated for quantification of opioids and unhydrolysed polar metabolites in autopsy specimens and was …
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Anti-inflammatory and anti-colon cancer potential of yerba mate (Ilex paraguariensis St. Hilaire) bioactive constituents
… the protective effects against colon carcinogenesis is not clearly understood. The objective of this study was to evaluate the dietary prevention of colon carcinogenesis by identifying novel constituents in yerba mate. The first aim was to investigate the potential anti-inflammatory effect of …
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