Global ETD Search
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Showing 1 to 19 of 19 for “"LAMP-1"”.
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Identification of Cathepsin B and L as Novel Uva Targets Upstream of Cutaneous Lysosomal-Autophagic Dysregulation
… of lysosomal associated membrane protein-1 (Lamp-1) and Lysotracker-based flow cytometric analysis. While lysosomal membrane integrity remained intact, autophagic blockade was suggested by accumulation of cellular protein levels of LC3-II and p62 (sequestosome 1) in UVA-exposed fibroblasts. …
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Signalbindung und Membraninteraktion von heterotetrameren Adaptorprotein-Komplexen
… Signale (YXXΦ) der lysosomalen Proteine LAMP-1, LAMP-2A, LAMP-2B, CD63 und des am TGN-lokalisierten Proteins TGN38 mittels Oberflächenplasmonresonanz-Analyse (Biacore) in vitro untersucht. Alle getesteten Signale interagierten kaum mit AP4, aber gut mit AP3. Die Bindung an AP1 und AP2 war …
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Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice
… of lysosomal associated membrane protein-1 (LAMP-1). LyEXO is a calcium-regulated physiological process which results in the fusion of lysosomes with the plasma membrane and consequent remodeling of the PM and release of lysosomal luminal content into the extracellular space. Defective …
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Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol
… transferrin receptor, and the lysosomal marker, lamp-1, were then immunostained with cy-5 conjugated secondary antibodies, and colocalization of bacteria with each marker was evaluated separately using confocal scanning laser microscopy (cslm). In both tissue models, colocalization of viable map …
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Analyse der Reifung von Afipien- und Rhodokokken-enthaltenden Phagosomen in Makrophagen
… das auch zu späteren Zeitpunkten negativ für LAMP-1 ist. Nur die circa 30 Prozent der Afipien, die sich in einem Kompartiment befinden, das zum endozytischen System gehört, gelangen nach der Aufnahme durch den Makrophagen in ein EEA1-positives Kompartiment, das zu einem späteren Zeitpunkt …
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Isolierung und Charakterisierung Neuromelanin-assoziierter Proteine aus Neuromelanin-Granula des menschlichen Gehirns mittels subzellulärer Proteomanalyse
… z.B. charakteristischen Membranproteinen (LAMP-1), sämtlichen Proteasen, Proteinen des Metabolismus von (Glyco-)Lipiden und Glycoproteinen, aber auch Proteinen des Cytosols und des vesikulären Verkehrs. Entscheidend war die Anwesenheit von Proteinen des Endoplasmatischen Reticulums (ER); …
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Investigation and Characterization of the Optical, Thermal, and Adsorption Properties of Carbon Aerogels for CO2 Capture Applications
… Mid-wave infrared thermography under a xenon arc lamp (1 Sun irradiance) quantified transient heating and cooling. Dynamic breakthrough experiments with a 11% CO₂ / 89% N₂ mixture at 25 °C, followed by photothermal desorption at 110 °C, were performed to determine CO₂ capacity and regeneration …
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Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells
… of lysosomal-associated membrane protein-1 (LAMP-1) and cathepsin D). Moreover, human primary cTBCs harboring S.Tm underwent rapid death of the cells. Infected cTBCs expressed phosphorylated-receptor-interacting serine/threonine-protein kinase (RIPK)-1 protein and phosphorylated-mixed lineage …
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Studies of intraorganelle dynamics: the lysosome, the pre-lysosomal compartment, and the golgi apparatus
… protein intermixing was shown by the transfer of LAMP-2, a mouse specific lysosomal membrane antigen, from mouse lysosomes to hamster sucrosomes, sucrose-swollen lysosomes. Lysosomal membrane protein intermixing was also shown by the co-localization of LIMP I, a rat specific lysosomal membrane …
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Cibler la vulnérabilité lysosomale dans le cancer du rein héréditaire comme nouvelle approche thérapeutique
… a été étudiée par immunofluorescence pour LAMP-1 à l’aide de la microscopie confocale, tandis que l’expression de protéines reliées à l’autophagie, les lysosomes et de plusieurs autres protéines a été étudiée par l’immunobuvardage de type Western. Nos résultats ont montré que les mutations …
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Light field microscopy for 3D imaging flow cytometry
… distribution of a type-1 membrane protein (LAMP-1) was imaged at multiple time points and analysed to reveal the heterogeneity in trafficking rate across large numbers of cells. In summary, this thesis charts the development, characterisation, optimisation, and application of a novel 3D …
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Untersuchungen zu intrazellulären Folgereaktionen von Neisseria meningitidis und Escherichia coli K1 in HBMEC (human brain microvascular endothelial cells)
… als Marker für das frühe Endosom und des Lamp-1 (lysosomal associated membrane protein 1) als Marker für das späte Endosom konnte durch Kolokalisationsstudien mittels Immunfluoreszenzmikroskopie gezeigt werden.
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Die MHC-Klasse-I-regulatorische Funktion des 48 kDa Glykoproteins des murinen Cytomegalovirus
… das ER und werden durch den Golgi-Apparat in ein Lamp-1+ Kompartment, höchst wahrscheinlich in die Lysosomen, transportiert und dort rasch abgebaut. Die Degradation ist sensitiv gegenüber verschiedenen endosomalen/lysosomalen Inhibitoren. Eine Hemmung führt zur Akkumulation von komplex …
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The physiological role of P2X4 receptors in lysosome function
… fusion in response to the agonist BzATP. LAMP-GECO was used to measure changes in cytosolic [Ca2+] within the vicinity of the lysosome. Fusion of the Ca2+ reporter (GECO) to the C-terminus of LAMP-1 targets GECO to the cytosolic surface of the lysosome. Co-expression of P2X4R with P2X7R …
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Analysis of the maturation of Rhodococcus equi-containing vacuoles in macrophages
… some late endosomal markers, such as Rab7, LAMP-1, and Lamp-2, they did not acquired vATPase, did not interact with pre-labeled lysosomes, and failed to acidify. These data clearly suggest that the RCV is a compartment which has left vacuoles that resemble multivesicular body compartments …
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Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins
… endosomal marker, Rab7, and lysosomal marker, LAMP-1, implicating bacterial-derived proteins in the ApV's altered fusogenicity. Therefore, we rationalized that A. phagocytophilum-encoded proteins that associate with the AVM may establish interactions with the host cell that are important for …
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Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
… sorting of lysosomal membrane proteins like Lamp-1 and LimpII is AP-3 regulated (Eskelinen et al., 2003). To study how AP-1A and AP-3 coats are stabilized on membranes and to identify the protein networks involved, a liposome based in vitro assay that recapitulates the fidelity of protein …
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Intracellular triggering of inflammation by the extracellular bacterium Pseudomonas aeruginosa
… by lysosomal-associated membrane protein (LAMP)-1-specific staining of the plasma membrane. We hypothesize a role for membrane potential in triggering of inflammasome activation by bacteria possessing a secretion system. Potassium-efflux has previously been identified as a activator of the …
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The Interactions of Clostridium Perfringens With Phagocytic Cells
Clostridium perfringens is the most common cause of gas gangrene (clostridial myonecrosis), a disease that begins when ischemic tissues become contaminated with C. perfringens. C. perfringens quickly multiplies in ischemic tissues and spreads to healthy areas, leading to high levels of morbidity …