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Showing 1 to 20 of 86 for “"L-lysine"”.
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Retention of fermentation biomass for extended L-Lysine fermentations
… thesis it was demonstrated that the current L-lysine fermentation technology can be enhanced by continuously withdrawing spent medium while recycling the biomass in the culture suspension to the bioreactor. The biomass in the reactor outlet stream is separated from the spent medium using …
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Branchpoint flux analysis in the L-lysine pathway of Corynebacterium glutamicum
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1997.
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Metabolic activity control of the L-lysine fermentation by restrained growth fed-batch strategies
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1992.
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Development of mouse morulae after encapsulation in alginate microgels or poly-l-lysine microcapsule
Three experiments were conducted to evaluate in vitro and in vivo development of zona pellucid a-intact (ZPI) and zona pellucida-free (ZPF) mouse embryos after encapsulation in either 2% sodium alginate or 0.1% poly-L-Iysine (PLL). In Experiment 1, rate of development of ZPI embryos (n = 150) from …
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The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa
… were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) did …
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… of GdnHCl with three model systems: poly-L-lysine (PLL), horseradish peroxidase (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the interpretation of the FTIR results, fluorescence and …
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Development and Application of a Microfluidic Cultivation Platform for the Analysis and Control of Industrially Relevant Synthetic Microbial Co-Cultures
… studied in a model consortium consisting of an L-lysine producing Corynebacterium glutamicum strain and an L-lysine auxotrophic C. glutamicum variant in spatially separated but nanochannel-connected cultivation chambers at the single-cell level. L-lysine secreted by the L-lysine producer could be …
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Dietary Methionine and Lysine Utilization by Chicks as Influenced by Coccidial Infection and Drug Therapy
… and narasin, and the dietary factors were L-lysine$\cdot$HCl, L-arginine, DL-methionine, protein level and source as well as dietary electrolyte balance. Digestibility and bioavailability of crystalline L-lysine$\cdot$HCl were also studied. Coccidial infection was produced via crop intubation …
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Conformational and Orientational Dynamics of Semi-Rigid Macromolecules in Shear Flow
… and phase stability of aqueous Poly-L-Lysine solutions in shear flow. Poly-L-lysine exists as an $\alpha$-helix at high pH and a random coil at neutral pH. When the $\alpha$-helix is heated above 27$\sp\circ$C, the macromolecule undergoes a conformational transition to a $\beta$-sheet. …
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Single label fluorescent derivatization of peptides
… group selectivity, using $\rm N\alpha$-t-BOC-L-lysine and $\rm N\varepsilon$-t-BOC-L-lysine as model compounds for derivatization through $\varepsilon$-amino and $\alpha$-amino groups, respectively. The optimal pH was found to be 8.5. Six small peptides, Arg-Lys, Lys-Trp-Lys, Lys-Lys, …
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Structural and Mechanistic Studies on N-Hydroxylating Monooxygenases Involved in Siderophore Biosynthesis
… catalyze the hydroxylation of L-ornithine or L-lysine. This is the first, committed step to siderophore biosynthesis. Pathogenic microbes including Aspergillus fumigatus and Mycobacterium tuberculosis secrete these low molecular weight compounds in order to uptake FeIII from their hosts for …
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Studies in the biosynthesis of virginiamycin S₁
… with either radioactive or stable isotopes. L-Lysine-U-¹⁴C was incorporated into both the 4-oxo-L-pipecolic acid and 3-hydroxypicolinic acid residues. The formation of the heterocyclic ring of both of these amino acids was shown to occur with retention of the nitrogen from the ε-amino group of …
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Non-electrolyte transport in brush border membrane vesicles from bovine small intestine
… for the two systems. Transport of L-proline, L-lysine, L-methionine, and L-phenylalanine were sodium stimulated. Data indicated sodium independent transport accounted for more influx of L-lysine, L-methionine, and L-phenylalanine than sodium dependent transport. Sodium dependent and sodium …
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Engineering semiconductor quantum dots for quantitative imaging of cell motility and invasion
… acid, and solely PEG, were incubated on poly-l-lysine, collagen, fibronectin, and gelatin coated glass coverslips. Based upon the emission intensity normalized by the quantum yield (QY), the binding of the QDs were directly compared. The QD coated substrates exhibited photoluminescent …
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The Engineering and Development of pH-Responsive Biopolymers for Drug Delivery Applications
… of pH responsive biocompatible poly (L-lysine iso-phthalamide) (PLP) polymer and its derivatives. Poly (L-lysine isophalamide) is a unique polymer that contains pendant carboxylate group and modification with phenylalanine was able to enhance the intracellular delivery efficiency of the …
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The activity of pH membrane disruptive pseudopeptides and their subcellular fate in mammalian cells cultured in-vitro
… were obtained by the copolymerisation of L-lysine and L-lysine ethyl-ester with various dicarboxylic acid moieties. In this way a linear polyamide comprising of alternating pendant carboxylic acids and pendant hydrophobic moieties was made. At physiological pH (pH 7.4), electrostatic …
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L-arginine overproduction in Corynebacterium glutamicum ATCC 13032
… of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving …
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