Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Kupffer cells"”.
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KUPFFER CELLS¿ MITOCHONDRIAL PLASTICITY AFFECTS SYSTEMIC IMMUNO-METABOLISM
BACKGROUND: Kupffer cells (KCs) are hepatic resident macrophages that are essential for liver physiology and contribute to the development of metabolic-associated fatty liver disease (MAFLD). Due to their high plasticity, KCs can maintain immunotolerance and mediate cell-cell interaction within the …
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Production of prostaglandin E2 and thromboxane A2 by rat liver macrophages and involvement of nitric oxide and cytokines in mediator pathways under inflammatory conditions
… hepatocytes as well as non-parenchymal liver cells like resident liver macrophages (Kupffer cells (KC)), Stellate cells and endothelial cells. Kupffer cells play a critical role in liver (patho)physiology and in the defense of the liver during inflammation. They constitute about 50% of …
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Metabolism of supplemental iron by hepatic parenchymal and sinusoidal cells of the neonatal pig
… livers by differential centrifugation, while Kupffer cells and endothelial cells were isolated by centrifugal elutriation. One day old piglets were injected with iron-dextran (Fe-dextran) and the concentration of accumulated iron was determined in all three cell types 1, 5, and 10 days later. …
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Study of Physiologic and Immunologic Incompatibilities of Pig to Human Transplantation
… were evaluated using liver macrophages, Kupffer cells, leading to identification of CD18, beta-2 integrin, as a potential target for modification. When disruption of CD18 was accomplished, human platelet binding and clearance by pig Kupffer cells was inhibited. Further, human and pig …
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Συσχέτιση ιστολογικών, βιοχημικών και αιματολογικών ευρημάτων μετά από χορήγηση LPS P. aeruginosa σε επίμυες wistar: Πειραματική μελέτη
… Adrenals with cytoplasmic vacuoles mainly in cells of the fascicular zone, and kidneys with necrosis of epithelial cells of the proximal convoluted tubules. Ultrastractural examination of the liver showed swollen mitochondria, dilated canaliculi, reduction of Hglycogen, prominent lysosomes and …
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All- trans-retinol modulation of chemically-induced pulmonary and hepatic toxicity
… of reactive oxygen species from retinol-primed Kupffer cells. However, in the lung it has been shown that retinol can protect against many of the inflammatory effects caused by bleomycin. Whether or not retinol pretreatment can modulate chemical-induced injury of compounds that cause both liver …
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PI3K/Akt activation is critical for early hepatic regeneration after partial hepatectomy
… (ie, 48h and 72h). Fewer macrophages and Kupffer cells were present in the regenerating liver of mice treated with wortmannin or siRNA to p85ƒÑ or p110ƒÑ, as reflected by a paucity of F4/80+ cells present by immunohistochemistry (IHC). Additionally, PI3K inhibition led to an aberrant …
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Towards Understanding Nanoparticle Uptake by the Liver: The Role of Flow Dynamics, Organ Microarchitecture and Cellular Phenotype
… In Aim 1, we isolated and analyzed liver cells from a quantum dot-treated animal. We report that not only Kupffer cells, but also sinusoidal endothelial cells and hepatic B cells are important for quantum dot clearance. In Aim 2, we identify three factors that determine nanoparticle …
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NONCANONICAL PYROPTOSIS PROMOTES NONALCOHOLIC STEATOHEPATITIS VIA LIPID PEROXIDATION AND TRAINED IMMUNITY
… macrophages (HMΦ’s), which include resident Kupffer cells and monocyte-derived macrophages, are the primary drivers of liver inflammation in both human and mouse models. In macrophages, chronic lipid exposure promotes pro-inflammatory polarization and the activation of pyroptosis via the …
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Use of gene expression to characterize heterogeneous liver cell populations
Non-parenchymal cells (NPC's) are integral to recreate the native hepatic microenvironment and necessary to maintain in vivo liver function. A variety of in vitro culture systems have been developed to address different aspects of liver physiology and architecture in order to recreate the …
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Development of high-throughput nanoparticle screening technologies to facilitate the delivery of genetic therapies to non-liver cell types
… clinical success as delivery methods to liver cells. In order to treat genetic diseases outside the liver, we hypothesized that a novel methodology for LNP discovery must be applied. Utilizing DNA barcodes to allow for the testing of >100 LNPs simultaneously, we determined that the correlation …
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Engineering Organoids for Stem Cell Maturation
… functions. However, sourcing these adult liver cells is extremely difficult since they are usually obtained through biopsies and are limited in quantity. Induced pluripotent stem cell (iPSC)-hepatocyte-like cells (iHLCs) hold tremendous potential to be used as a substitute since they can be …
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Prevention of Ischemia-Reperfusion Injury in the Rat Liver by Atrial Natriuretic Peptide
… comprised hepatocytes and sinusendothelial cells predominantly in the periportal liver areas. As target molecules for ANP action, the cGMP-dependent protein kinases did not seem to be responsible for the conferred cytoprotection. In the liver, no expression of these kinases could be detected …
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Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
… with non-parenchymal cholangiocytes, endothelial cells, Kupffer cells, and hepatic stellate cells. Disorders affecting hepatocytes are life threatening and increasing in prevalence12, with end-stage treatment relying upon a limited supply of liver donations for transplant. Consequently, the …
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The Design of Three-Dimensional Multicellular Liver Models Using Detachable, Nanoscale Polyelectrolyte Multilayers
… rat hepatocytes, liver sinusoidal endothelial cells (LSECs), and Kupffer cells (KCs). LSECs and KCs in the liver model were separated from hepatocytes by a nanoscale, detachable, optically transparent chitosan and hyaluronic acid (HA) polyelectrolyte multilayer (PEM) film. The properties of the …
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Investigating the Heterogeneity of Tumor Associated Macrophages in Human Hepatocellular Carcinoma
… are transcriptionally distinct from canonical Kupffer cells and exhibit a higher composition of C1QA+Marcolo population. Macrophages in the HCC liver also had an increased expression of regulatory surface markers (such as CD206 and PDL1) and functional phenotype (decreased TNF-α secretion after …
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Analysis of the Metabolism of Dextran-Methylprednisolone Succinate Using
… with 2.5% rat serum. Separation of rat liver cells was accomplished by perfbsion of the liver with HEPES buffer containing collagenase, followed by centrihgation through a density gradient of Percoll to yield populations of hepatocytes (93% pure, with 80% viability) and Kupffer cells (98% …
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Engineering complex liver models for drug screening and infectious diseases: a biomaterials and co-culture perspective
… human hepatocytes (PHHs) and supportive stromal cells have been shown to display high hepatic functions for long-term drug and disease studies. However, MPCCs lack liver non-parenchymal cells (NPCs) and the proper microenvironmental cues that can play important roles in conditions such as …
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The role of caspase-1 in liver and adipose tissue during metabolic dysregulation in mouse models on NASH
… was detected in hepatocyte and non-parenchymal cells. However, after feeding mice the MCD diet, fractionation of the liver further demonstrated an increase of caspase-1 and interleukin-1¿¿ only in the non-parenchymal fraction. Casp1-/- mice on the MCD diet were protected from diet-induced …
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
… indicated that HLOs comprise of 60,4% EpCAM+ cells, 11,2% EpCAM-/CD166+ cells and 5% EpCAM-/CD68+ cells, respectively. HLOs exhibited high CYP3A4 enzyme activity compared to HepaRG 3D model. Proinflammatory (IL-6, IL-1β and TNF-α) and anti-inflammatory (IL-4 and IL-10) cytokines were elevated …
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