Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Ku heterodimer"”.
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Shedding Light on the Biological Effects of Ionising Radiation on DNA Using Advanced Optical Microscopy
… resulted in increased foci persistence. Although Ku70/Ku80 are known as major effectors in DSB repair and genome integrity, their interaction in relation to DSB repair has not been detected in living cells. Using GFP technology and advanced imaging, Ku70-80 interaction was shown for the first time …
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Investigating the Molecular Mechanisms Underlying Ku Essentiality in Human Cells
The Ku heterodimer is composed of two subunits, Ku70 and Ku80, and is well- known for its role in nonhomologous end-joining DNA repair. Ku has also been associated with noncanonical functions in the cell for which its mechanisms are not fully understood such as its roles in telomere maintenance, …
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Functional Analysis of the Yku Complex in Telomere Length Regulation
The Ku protein from Saccharomyces cerevisiae (Yku) forms, like its human homologue hKu, a heterodimer comprised of a 70kD and an 80 kD subunit. In yeast and mammals, the Ku heterodimer is required for the repair of DNA double strand breaks (DSBs) via nonhomologous end-joining (NHEJ). Interestingly, …
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Investigations into the vaccinia virus immunomodulatory proteins C4 and C16
… as well. Like C16, C4 associates with the Ku heterodimer to block its binding to DNA leading to reduced production of cytokines and chemokines. For both proteins, the function localised to the C termini and was abrogated by mutating three residues. Secondly, C16 induces a hypoxic response …
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Development of monoclonal antibodies for the identification of novel invasion associated targets in human cancer.
… and 80kDa. LC-MS/MS analysis identified these as Ku70 and Ku80 respectively, two subunits of the Ku heterodimer, which is involved in DNA double strand break repair. siRNA silencing of these two subunits in the MiaPaCa-2 clone 3 and DLKP-M cell lines significantly reduced levels of invasion and …
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Cryo-EM as a Tool to Study the Diverse Interactome of Ku 70/80 in NHEJ and Beyond
… Briefly, DSBs are initially recognised by the Ku 70/80 (Ku) heterodimer (6), which subsequently recruits DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to form the DNA-PK holoenzyme complex, to mediate synapsis of the broken DNA ends (7, 8). Depending on the DNA end configuration, …