Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Krab"”.
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Deep vertebrate roots for mammalian krab zinc-finger transcription factors
KRAB-associated C2H2 zinc-finger (KRAB-ZNF) proteins are the products of a rapidly evolving gene family that traces back to early tetrapods, but which has expanded dramatically to generate an unprecedented level of species-specific diversity. While most attention has been focused on the more …
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Characterising the interaction between TRIM28 and members of the Krab-ZFP family in transcription silencing
… box-containing zinc finger proteins (Krab-ZFPs). Silencing is initiated by the recruitment of TRIM28 to a target locus by members of the Krab-ZFP. Subsequently, TRIM28 functions as a scaffold protein to recruit chromatin modifying effectors featuring SETDB1 (SET domain bifurcated …
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The Estrogen Response Element and the KRAB Repression Domain as Modulators of Estrogen Receptor Action
… ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand …
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The KRAB Zinc Finger Protein ZKSCAN3 binds to a SINE-embedded regulatory sequence to repress enhancer activity
KRAB Zinc Finger Proteins (KZFPs) are the largest family of transcription factors in mammals and are generally associated with the binding and repression of transposable elements. KZFPs use their KRAB domain to recruit the cofactor KAP1, which acts as a scaffold for the induction of …
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Primate-specific KRAB zinc finger proteins and their targets rewire regulatory networks related to inflammation and pancreas development
… subfamily of DNA binding factors in human, KRAB Zinc Finger proteins (KZFPs), leads to the epigenetic silencing of their bound loci and have been recently shown to mainly target repetitive regions of the genome, mostly TEs. Interestingly most TEs targeted by KZFPs have lost their ability to …
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Molecular mechanism of KAP1-dependent transcriptional silencing
… of potentially harmful retroelements is KRAB-associated protein 1 (KAP1, also known as TRIM28 or TIF1β). Following its recruitment to retrotransposons by sequence-specific KRAB domain-containing zinc finger proteins (KRAB-ZFPs), KAP1 induces the assembly of an epigenetic silencing …
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Selection and Characterization of a Steroid Receptor DNA Binding Domain-Based Repressor of Estrogen Response Element Containing Promoters
… with a flexible linker and fused it to a strong KRAB repressor domain. The dimerized FLAGDBD5-KRAB (FdDBD5K) is a much more potent repressor than the monomer FLAGDBD5-KRAB (FDBD5K). The FdDBD5K elicited significant repression of both constitutive and estrogen receptor induced transcription on a …
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Unlocking Compacted Heterochromatin Through Modulation Of Associated Proteins
… In an additional study, I investigated how KRAB domain-containing zinc finger proteins (KRAB-ZFPs) might silence hepatic genes in alternate lineages. I identified six putative liver lineage antagonistic KRAB-ZFPs. A mostly unstudied primate-specific KRAB-ZFP known as ZNF695 became the top …
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Comparison of orthologs across multiple species by various strategies
… yet. For example, the vertebrate roots of the KRAB-ZNF family in particular and of polydactyl ZNF genes in general, remain somewhat mysterious. In addition, due to its repeating gene structure and the fact that they often reside as clusters, these genes can be difficult to model correctly with …
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Gene duplication and alternative splicing play a role in modulating the functions of the ZNF286A transcription factor
… in ZNF286B codes for the chromatin-interacting KRAB-domains that are present in the ZNF286A gene; in this respect the new human paralog resembles a natural KRAB-less alternative isoform that we demonstrate to be expressed naturally from the parental ZNF286A gene. Using ChIP and siRNA knockdown, …
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
… and accelerated by persistent binding of a KRAB-containing transcriptional repressor. Furthermore, additional repetitive elements (LINE and SINE) located adjacent to the promoter could confer DNA methylation spreading into the CGI particularly in the setting of KRAB-factor binding. However, …
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Functional Analysis of KAP1 Requirements for HIV-1 Transcription and Latency Reactivation
… One such regulator is Krüppel-associated box (KRAB)-associated protein 1 (KAP1: also named TRIM28 or TIF1β). While initial studies have revealed KAP1 to be a positive regulator of latency reversal in transformed and primary CD4+ T cells, subsequent studies have proposed KAP1 to be a repressor …
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An integrated CRISPR-Cas toolkit for engineering human cells
… VP64 and mammalian transcriptional repressor KRAB, we build and characterize tunable, multifunctional and orthogonal CRISPR/Cas transcription factors (CRISPR-TFs) in human cells. By integrating CRISPR-TFs and Cas6/Csy4 based RNA processing with multiple mammalian RNA regulatory strategies …
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Delineating the Mechanisms Through Which ZNF165 Supports Growth and Survival of Triple-Negative Breast Cancer
… functions. In addition, I identified the KRAB zinc finger protein, ZNF446, and its associated tripartite motif protein, TRIM27, as obligate components of the ZNF165-SMAD3 complex that also support tumor cell viability. I found that while ZNF446 is bound to chromatin with the ZNF165-SMAD3 …
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A TRIM28 centric strategy to efficiently uncover the contribution of KZFPs to the evolution of gene regulatory networks
The family of KRAB zinc-finger proteins (KZFPs) is the largest group of DNA-binding factors in tetrapods and is rapidly evolving, with >350 protein-coding members in humans. While the individual roles of most human KZFPs is poorly characterized, it is known that their primary role is to …
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Characterisation of the non-canonical zinc finger protein ZFP263 in mouse
… that influence this in human and mouse is the KRAB zinc finger protein (KZFP) family. KZFPs are thought to have rapidly evolved alongside transposable elements and be mediators of transcriptional repression. The few KZFPs that have been characterised so far have been shown to be involved in a …
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Integration Sites in the Persistence of Latent HIV-1
… likely to be mapped to Krüppel-associated box (KRAB) domain-containing zinc finger (ZNF) genes on chromosome 19. Transcriptional and epigenetic meta-analysis of primary CD4+ T cells reveal that these specific chromosomal locations harboring integrated provirus are associated with genes …
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Identification and characterisation of KZFPs directly regulated by p53
… our genome across evolutionary history. KRAB zinc finger proteins (KZFPs) are the largest group of DNA binding factors in the human genome and mostly target TEs, controlling chromatin accessibility by effecting epigenetic changes at their binding sites which result in a localized …
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Developmental and Cellular Functions of Delta-Catenin
… its interaction with an uncharacterized KRAB family zinc finger protein I named ZIFCAT. My results indicate that ZIFCAT is nuclear, and suggest that it may associate with DNA as a transcriptional repressor. I further determined that other p120 sub-class catenins are similarly cleaved by …
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Using a novel large-scale epigenetic analysis method to uncover the cis-regulatory potential of individual transposable elements
… potential. One such mechanism is orchestrated by KRAB zinc finger proteins (KZFPs). These form the largest family of DNA binding factors in tetrapods, with more than 350 protein-coding members in humans. While little is known about their individual function, they have a recognised general role in …
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