Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 33 for “"Knock-out mouse"”.
-
Early onset of age related hearing loss in LDHB knock out mouse
… study of Age-related hearing loss in LDHB knockout mice. In response to deletion of LDHB gene in mouse caused early onset of age related hearing loss, as increase of high frequency threshold, hair cell death, which are the typical features of Age-related hearing loss. We investigate the …
-
Molecular analysis of decay accelerating factor as a potential susceptibility factor to developing treatment resistant extraocular muscle involvement in Myasthenia Gravis
… of complement pathways. The absence of DAF, in knock-out mouse models, has been shown to significantly increase the susceptibility to experimental autoimmune MG. A previous study showed that a high proportion of South African MG patients of African genetic ancestry develop immunosuppressive …
-
ELUCIDATING REGULATORY NETWORKS PROMOTING B-CELL DEVELOPMENT
… we have re-evaluated previously characterized knock-out mouse models in order to obtain a higher resolution analysis of critical events in early B-cell commitment. Based on these studies, we propose a transcriptional hierarchy where the transcription factors E2A and HEB initiate the B-cell …
-
Force-extension of the Amylose Polysaccharide
… of complement pathways. The absence of DAF, in knock-out mouse models, has been shown to significantly increase the susceptibility to experimental autoimmune MG. A previous study showed that a high proportion of South African MG patients of African genetic ancestry develop immunosuppressive …
-
The roles of DPPA2, DPPA4 and SMARCA5 in mouse zygotic genome activation, epigenetic reprogramming and development
… 2 cell stage. Previous screens in the lab, using mouse embryonic stem cell (mESC) identified the small DNA binding proteins DPPA2, DPPA4 and the chromatin remodeler SMARCA5 as potential inducers of major ZGA. Here I focus on validating these three factors and I assess their roles in vivo, using …
-
Hormonal regulation of phosphodiesterase 3B in adipocytes
… activation of PDE3B we used primary rat and mouse adipocytes, 3T3-L1 adipocytes and caveolin-1 knock out mouse adipocytes. The study on PDE3B phosphorylation shows that PDE3B is multisite phosphorylated in response to both insulin and catecholamines. We were able to identify six …
-
Die Rolle der Thrombozyten- und Leukozyten-Endothelzell-Interaktion in der Atherogenese
… in atherogenesis - an in vivo-study in the ApoE-knock-out-mouse - Platelets play a crucial role in thromboembolic complications of advanced atherosclerotic lesions but their involvement in the initiation of the atherosclerotic process in unclear. Platelet adhesion regulates secretion of …
-
Yellow Fever: Role of Heterologous Flavivirus Immunity on Urban Emergence
… Next, we utilized an interferon α/β receptor knock-out mouse model to determine the role of pre-existing DENV-2 and ZIKV immunity in YF virus infection, and to determine mechanisms of cross-protection. We utilized African and Brazilian YF strains and found that DENV-2 and ZIKV immunity …
-
Mechanisms of p19Arf-Mediated Regulation of Perivascular Cell Biology During Mammalian Eye Development
… space and serves to nourish the lens and retina. Knock-out mouse models for p19Arf demonstrate that in the absence of Arf, there is an abnormal accumulation of cells that persist into the adult secondary vitreous and cause detrimental ocular defects including blindness, retinal detachment and lens …
-
Phenotypic characterisation of the tremor mutant and AAV mediated aspartoacylase gene transfer in the rat model of Canavan disease
… phenotypes with both human patients and to the knock-out mouse model. The deletion of aspartoacylase results in a total loss of the capacity to metabolize N-acetyl-aspartate to acetate and aspartate in brain, leading to elevations in brain N-acetyl-aspartate levels, changes in cell and tissue …
-
Prevalence of Premature Ovarian Failure In Women With Tuberous Sclerosis
… the use of ovarian specific conditional knock-out mouse models has demonstrated a crucial role of the TSC genes in ovarian function. Mice with complete deletion of <em>Tsc1</em> or <em>Tsc2</em> showed accelerated ovarian follicle activation and subsequent premature follicular depletion, …
-
HEDGEHOG SIGNAL TRANSDUCTION IN EFFECTOR IMMUNE CELLS
… receptor engagement. Using a conditional knock-out mouse model and pharmacological blockade, I find that the Hh pathway is critical for tumour cell killing in murine and human NK cells. Mechanistically, the Hh pathway impacts the kinetics of NK immune synapse formation, without affecting …
-
Hypothalamic Nutrient Sensing
… imaging of adult neuron cultures from the mouse mediobasal hypothalamus, I demonstrated that leucine bidirectionally regulates neuronal activity in a neurochemically heterogeneous population of neurons, including AgRP/NPY and POMC neurons. Using pharmacological tools, I demonstrated, …
-
The Role of Slow-Twitch Skeletal Muscle in the Musculoskeletal Endocrine Axis
… using osteocyte-specific Mbtps1 conditional knock-out mouse (cKO) using Dmp1-driven Cre. MBTPS1 is a widely expressed proprotein convertase involved in proprotein processing within the secretory pathway. In bone, MBTPS1 is required for normal skeletal development and mineralization. Ex vivo …
-
Aspects of T cell development contributing to autoimmunity.
… resistance in the non-obese diabetic (NOD) mouse. The role of thymic expression of TGF-β was dissected by using TGF-β type II receptor cre/lox conditional knock out mouse model. By transplanting bone marrow to irradiated recipients we restricted the phenotype to hematopoietic stem cells. …
-
Dissecting the function of MYEF2 in neural stem cells and glioblastoma
… cell containing regions. CRISPR/Cas9 mediated knock-in of mCherry and HA to Myef2 was successfully carried out in mouse NSCs. We observed that MYEF2 is a nuclear protein which retains nuclear localisation and expression in both proliferative and quiescence NSCs, as well as differentiating …
-
Vascular Disease Pathogenesis In Smooth Muscle Dysfunction Syndrome and Majewski Osteodysplastic Primordial Dwarfism Type Ii
… dysfunction of smooth muscle cells (SMCs) throughout the body. Complications of SMDS include early-onset thoracic aortic aneurysms and dissections, moyamoya-like cerebrovascular disease, patent ductus arteriosus, pulmonary hypertension and poorly characterized pulmonary disease, hypoperistalsis of …
-
Exploring Dystrophin-Mediated Control of Neural Stem Cell Fate Associated with Intellectual Disability In Duchenne Muscular Dystrophy Patients
… used animal model to study DMD is the mdx mouse which completely lacks Dp427 but no other DMD isoforms. Although the muscle phenotype is well-established, behavioural characterization of the mdx mouse model has been inconclusive. In this thesis I investigated the hippocampal and amygdala …
-
Computational Analysis of Transcriptional Regulation
… viral integrants. Integration occurs without disrupting host 3D chromatin structure but alters the expression of many neighbouring host genes. The advent of reliable protocols for performing single-cell RNA sequencing (scRNA-seq) has revealed that transcriptional noise is widespread and a …
Page 1 of 2