Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"Klenow Fragment"”.
-
Investigation Into The Binding Interactions Of Klenow Fragment To Dna Modified With Carcinogens Af And Aaf Using Surface Plasmon Resonance
… of exonuclease deficient E. coli polymerase I, Klenow fragment (KF), to DNA modified with AF or AAF at two locations: as a templating base or in the last formed base pair. KF binding to the modified DNA bases was also monitored to DNA that contained a terminal mismatch. When the templating …
-
New insights into the mechanism of dna replication on unmodified and benzo[a]pyrene modified templates using surface plasmon resonance
… used to study the effect various dNTPs have upon Klenow fragment binding to several (-)-trans-anti-B[a]P-N2-dG adducted primer-templates. Binding constants were determined for Klenow fragment binding to primer templates containing either a correctly paired C:G-B[a]P, mismatched T:G-B[a]P, or …
-
Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
… by participating in DNA repair processes. The Klenow fragment of E. coli DNA polymerase I has served as a model polymerase for decades because it is straightforward to purify, well-characterized kinetically, and it is able to carry out DNA synthesis and proofreading. In 2009, Christian, et al. …
-
Poly(glutamic acid) promoted assembly of nucleosome cores on the histone gene quintet of psammechinus miliaris
… DNA at single base pair resolution, a series of fragments, excised from the H2A-Hl and the Hl-H4 spacer areas, were inserted into pGV403 plasmids. These plasmids can be strand specifically end-labelled with the Klenow fragment at the two different Tth 111 I excision sites utilised to isolate the …
-
Synthetic approaches to the understanding of DNA nucleobase methylation
… dG (^{7Me7C}dG) and 7-deaza- dG (^{7C}dG) by Klenow fragment of E. coli DNA polymerase I is examined. The misincorporation frequencies on the ^{7Me9C}dG, ^{7Me7C}dG, and 7CdG templates are comparable to the dG template, although the 7-methyl group slows down the turnover rate of the polymerase …
-
Expression Profiling by DNA Microarrays : Development of Amplification Methods for the Analysis of Minimal Tumor Samples
… followed by dye-incorporation using the Klenow fragment of E. coli DNA polymerase I generates fluorescent antisense cDNA. This protocol provides high fidelity and up to 105-fold amplification, starting from 2 ng total RNA. The generated data are highly reproducible and maintain relative …