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Showing 1 to 12 of 12 for “"Klenow"”.
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Investigation Into The Binding Interactions Of Klenow Fragment To Dna Modified With Carcinogens Af And Aaf Using Surface Plasmon Resonance
… of exonuclease deficient E. coli polymerase I, Klenow fragment (KF), to DNA modified with AF or AAF at two locations: as a templating base or in the last formed base pair. KF binding to the modified DNA bases was also monitored to DNA that contained a terminal mismatch. When the templating …
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… to their sequence and structural similarities, Klenow and Klentaq, the “large fragments” of the Pol I DNA polymerases from <I>Escherichia coli</I> and <I>Thermus aquaticus</I>, are considered functional homologues. Klentaq, however, does not have a functional proofreading site. Examination of …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
… aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then compared to their known crystal structures to assess the …
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New insights into the mechanism of dna replication on unmodified and benzo[a]pyrene modified templates using surface plasmon resonance
… used to study the effect various dNTPs have upon Klenow fragment binding to several (-)-trans-anti-B[a]P-N2-dG adducted primer-templates. Binding constants were determined for Klenow fragment binding to primer templates containing either a correctly paired C:G-B[a]P, mismatched T:G-B[a]P, or …
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Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
… by participating in DNA repair processes. The Klenow fragment of E. coli DNA polymerase I has served as a model polymerase for decades because it is straightforward to purify, well-characterized kinetically, and it is able to carry out DNA synthesis and proofreading. In 2009, Christian, et al. …
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Poly(glutamic acid) promoted assembly of nucleosome cores on the histone gene quintet of psammechinus miliaris
… can be strand specifically end-labelled with the Klenow fragment at the two different Tth 111 I excision sites utilised to isolate the propagated insert. On the free linearised DNA a complex digestion pattern is produced due to the sequence specificities of Micrococcal nuclease and DNase I. When …
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Assessment of LS-DYNA and Underwater Shock Analysis (USA) Tools for Modeling Far-Field Underwater Explosion Effects on Ships
This thesis investigates the use of the numerical modeling tools LS-DYNA and USA in modeling general far-field underwater explosions (UNDEX) by modeling a three-dimensional box barge that is subjected to a far-field underwater explosion. Past UNDEX models using these tools have not been validated …
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Finite and Spectral Element Methods for Modeling Far-Field Underwater Explosion Effects on Ships
The far-field underwater explosion (UNDEX) problem is a complicated problem dominated by two phenomena: the shock wave traveling through the fluid and the cavitation in the fluid. Both of these phenomena have a significant effect on the loading of ship structures subjected to UNDEX. An approach to …
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Environmental health risks, inequality and welfare beyond GDP
… welfare measure developed by Jones and Klenow (2016) to include these risks. We use the GBD estimates of environmentally related morbidity as a lower bound estimate of these risks to adapt the expected lifetime component of the Jones-Klenow welfare measure for each country relative to …
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Synthetic approaches to the understanding of DNA nucleobase methylation
… dG (^{7Me7C}dG) and 7-deaza- dG (^{7C}dG) by Klenow fragment of E. coli DNA polymerase I is examined. The misincorporation frequencies on the ^{7Me9C}dG, ^{7Me7C}dG, and 7CdG templates are comparable to the dG template, although the 7-methyl group slows down the turnover rate of the polymerase …
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Expression Profiling by DNA Microarrays : Development of Amplification Methods for the Analysis of Minimal Tumor Samples
… followed by dye-incorporation using the Klenow fragment of E. coli DNA polymerase I generates fluorescent antisense cDNA. This protocol provides high fidelity and up to 105-fold amplification, starting from 2 ng total RNA. The generated data are highly reproducible and maintain relative …
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Market frictions, allocative efficiency and aggregate productivity in the manufacturing sector: evidence from Zimbabwe
… chapters. Chapter 2 draws on the Hsieh & Klenow (2009) approach to measure the extent of allocative inefficiency within and between the formal and informal manufacturing sector firms in Zimbabwe. The results reveal a high degree of misallocation that is more pronounced in informal sector …