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Showing 1 to 20 of 123 for “"Kinesin"”.
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Microtubule interactions and regulation of the mitotic kinesin-like protein-1 and kinesin-like calmodulin-binding protein
… Microtubules operate in conjunction with the kinesin superfamily of microtubule-dependent motor proteins, which use the energy from ATP hydrolysis to "walk" along microtubule tracks, and in doing so generate force for the transport of cellular cargo and mitosis. The goal of this project was to …
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Structural Basis for Coordination in Dimeric Kinesin
Kinesin-1 (conventional kinesin) is a protein motor that carries organelles and vesicle cargo along its microtubule track. The two catalytic heads of Kinesin-1 are linked to function as a highly processive "molecular walker'' that can take hundreds of steps before falling off the track. A key …
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Kinesin-1 mechanical flexibility and motor cooperation
Conventional kinesin (kinesin-1) transports membrane-bounded cargos such as mitochondria and vesicles along microtubules. In vivo it is likely that several kinesins move a single organelle and it is important that they operate in a coordinated fashion so that they do not interfere with each other. …
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Small-Molecule Control of Kinesin-5 Proteins
… the "work" within the apparatus. In particular, Kinesin-5 motor proteins, a subset within the kinesin motor protein superfamily, are primarily responsible for organization of microtubules (MTs) within the mitotic apparatus, and are consequently vital for efficient mitosis. These proteins utilize …
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KINESIN-MICROTUBULE INTERACTIONS DURING GLIDING ASSAYS UNDER MAGNETIC FORCE
Conventional kinesin is a motor protein capable of converting the chemical energy of ATP into mechanical work. In the cell, this is used to actively transport vesicles through the intracellular matrix. The relationship between the velocity of a single kinesin, as it works against an increasing …
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Experimental and theoretical investigation of mechanism of Kinesin motility
Kinesin is a motor protein capable of utilizing chemical energy from ATP hydrolysis to generate mechanical force to power its progressive motility along a microtubule track. The mechanism of motility has been a subject of extensive study for last decade. Recently, it has been proposed that novel …
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Tools to study the kinesin mechanome using optical tweezers
… by which molecular motor function has been the kinesin family. Missing, however, is a clear understanding of the series of events that take place at the atomistic level when kinesin walks on a microtubule and generates force. Recent MD simulations have identified the force-generating mechanism …
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Force studies of multiple kinesin-1 and EG5 molecular motors
Kinesin-1 and Eg5 are molecular motors that are essential for eukaryotic cell growth and development. Kinesin-1 motors transport intracellular cargo along the microtubule cytoskeleton through and against the dense viscoelastic cytoplasm. Eg5 motors mediate proper mitotic, microtubule spindle …
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Single-molecule studies of protein degradation and kinesin-8 motility
… two biological systems: ClpXP proteases and kinesin-8 Kif18B. As members of bigger protein families, these two fascinating nanomachines perform important cellular tasks in protein quality control and spindle formation, respectively. AAA+ ClpX unfoldases use energy from ATP binding and …
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Characterization and cooperative dynamics of kinesin motor proteins in intracellular transport
… a modular plasmid toolkit for controlled kinesin expression and purification, and I created a novel DNA origami scaffold that allows motors to be positioned with defined stoichiometry and geometry on a rigid cargo-like platform. Together, these advances provided a programmable experimental …
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How Kinesin-1 Deals With Roadblocks: Biophysical Description and Nanotechnological Application
… on biotinylated microtubules, it was shown that kinesin-1 motors first stop and then detach when they encounter a streptavidin obstacle on their path along the microtubule. Consequently, the deceleration of streptavidin coated microtubules in gliding assays could be attributed to an obstruction …
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Regulation of Kinesin-3 activity by active zone protein SYD-2
… auf die Charakterisierung des Effekts der SYD-2/Kinesin Interaktion auf die Motilität und mechanochemische Eigenschaften des UNC-104 Motors.In vitro können die Motilitätseigenschaften von molekularen Motoren am besten mittels rekombinant exprimierten Motoren, welche an fluoreszierende Proteine …
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Studies of Huntington's disease associated motor domain phosphorylation of kinesin-1
… conserved residue found in all three isoforms of kinesin-1 in impaired axonal transport associated with Huntington’s disease. Phosphorylation adds both negative charge and bulk to a protein. The mechanism by which S175 modification is related to impaired transport is not very well understood. It …
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Leveraging the motor protein Kinesin to manipulate DNA molecules in synthetic environment
… Ihr Ziel ist es, das Motorprotein Kinesin und Mikrotubuli einzusetzen, um DNS-Moleküle in einem synthetischen Umgebung zu manipulieren. Diese Doktorarbeit setzt sich aus fünf Kapiteln zusammen. In der Einführung wird die makromolekulare Struktur der Zelle beschrieben, z.B. das …
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Biology's wires and motors : single-molecule mechanics of M13 bacteriophage and kinesin
… systems - M13 filamentous bacteriophage and kinesin motor protein - and the properties of their underlying parts. M13 is a filamentous virus that has elicited the interest of the engineering community for its use in phage display of combinatorial peptide libraries and more recently as a 1D …
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Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1
The motor proteins dynein and kinesin are fascinating biological machines which, like vehicles in a city, move various cellular cargoes along cytoskeletal microtubules (MT). In filamentous fungi, these motors are important for the hyphal growth, which is characterized by localized extension at the …
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Gates and Latches: the mechanochemistry of ATP binding in kinesin 1 and Eg5
… cargo transport along microtubule filaments by Kinesin 1. A gating mechanism is needed to coordinate ATP binding to the two catalytic domains within Kinesin 1 in order to prevent simultaneous binding, hydrolysis and detachment of both heads which could prematurely terminate cargo transport. The …
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A Novel Collapsing Response Mediator Protein 2 Binding Domain for Kinesin Light Chain 3
… is not clear, but was proposed to involve kinesin proteins. KLC3 is the only known kinesin light chain expressed in elongating spermatids that develop the flagellum. In this study, we investigated the expression of CRMP2 in mouse testis and we studied if CRMP2 and KLC3 interact. We observed …
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Negative interference in systems of coupled kinesin: A study of self-assembling complexes with defined structure
… that allows structurally-defined complexes of kinesin-1 to be created. This approach also provides synthetic control over intermotor spacing and the elasticity of the mechanical motor linkages to rigorously characterize the effects of system structure on the interactions of exactly two motors. …
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The characterisation of family-13 kinesins in Trypanosoma brucei
Kinesins are motor proteins involved in the movement of organelles and sub-organelles along microtubule tracks within the cell. Phylogenetic analysis of the 46 kinesin genes coded by the Trypanosoma brucei genome resulted in the grouping of seven kinesin sequences into the Kinesin-13 family. …
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