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Showing 1 to 20 of 21 for “"Kinase substrate"”.
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Identification of a protein kinase substrate in Sulfolobus solfataricus P2
… products were probed with a purified protein kinase, SsoPK4, and radiolabeled ATP to identify potential native substrates. A protein fragment of the ORF sso0563, the catalytic A-type ATPase subunit A (AtpA), was phosphorylated by SsoPK4. Full length and truncated forms of AtpA were …
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Synthesis And Application Of Atp Analogs For Phosphorylation-Dependent Kinase-Substrate Crosslinking
… of signaling cascades and cellular functions. Kinases phosphorylate protein substrates in a highly regulated manner and are promiscuous. Understanding kinase-substrate specificity has been challenging and there is a need for new chemical tools. To this end we developed -phosphate modified ATP …
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Statistical methods to infer kinase activities and kinase-substrate interactions using phosphoproteomic data
The activity of kinases play an important role during cell signalling. They phosphorylate proteins to regulate their interactions, structural conformation, cell location and enzymatic activation. Mass Spectrometry (MS) based phosphorylation studies (i.e. phosphoproteomics) has revolutionized the …
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Analysis of the understudied parts of the phospho-signalome using machine learning methods
… through adding phosphoryl groups onto substrates such as lipids or proteins, which in turn leads to changes in substrate function. Crucial components of this system include kinases, which phosphorylate on the substrate molecule and phosphatases that remove the phosphoryl group from the …
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Engineering orthogonal signaling pathways to probe sequence space capacity
… 58 new insulated pathways, in which functional kinase-substrate pairs have different specificities than the parent proteins, and demonstrate that several new pairs are orthogonal to all 27 paralogous pathways in E. coli. Additionally, I readily identify sets of 6 novel kinase-substrate pairs …
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Design, Synthesis and Evaluation of Diphenylamines as MEK5 Inhibitors
The mitogen-activated protein kinases (MAPK) are a family of interrelated signal transduction kinases mediating intracellular responses to extracellular events. They are involved in mediating complex cellular events including, cell differentiation, cell proliferation, and cell death. Extracellular …
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Regulation of Cell Invasion by the Podosome/Invadopod Protein Tks5
Tks5 is a Src tyrosine kinase substrate and scaffolding protein for specialized cytoskeletal structures called podosomes/invadopodia. My doctoral thesis has focused on how Tks5 regulates the formation of these related structures and their impact on the invasive phenotype of cells. The podosomes of …
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Expression of the P-glycoprotein Homologue1 on food vacuoles isolated from Chloroquine-sensitive and resistant Plasmodium falciparum strains
… food vacuoles indicated that Pgh1 is not a major kinase substrate.
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In-depth bioinformatics analysis of the phosphoproteome of triple negative breast cancer treated with a tumor selective NQO1 bioactivatable drug
… Computational and bioinformatic analysis of kinases involved through kinase substrate enrichment analysis revealed changes in downstream signaling of cell cycle checkpoint proteins. CDK1 and CDK2 substrate phosphorylation was decreased in response to combination drug therapy. Based on the …
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Regulation of PKCalpha and the role of PKC in neuroblastoma cell migration
Members of the protein kinase C (PKC) family of serine/threonine kinases play critical roles in cellular regulation. Depending on differences in regulation, PKCs can be divided into classical (alpha, betaI, betaII and gamma), novel (delta, epsilon, eta and theta) and atypical (zeta and iota/lambda) …
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Development of Tyrosine Kinase Peptide Biosensors and Methods for Detection
<p>New methods to monitor tyrosine kinase activity are critical for studying kinases in cell biology, drug discovery and the clinic. Peptide-based biosensors for detection of kinase activity utilitize a kinase specific artificial peptide substrate, which can report intercellular kinase activity …
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MECHANISM OF PINK1-MEDIATED UBIQUITIN PHOSPHORYLATION
… phosphorylation by PINK1 (PTEN-induced Putative Kinase 1) is crucial for mitochondrial quality control and loss or mutation of PINK1 can lead to autosomal recessive juvenile parkinsonism (AR-JP). PINK1 is an unusual kinase, as it is characterised by three unique insertions in its kinase N lobe …
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Implication des protéines adaptatrices Tks4 et Tks5 dans la dégradation du cartilage articulaire par les synoviocytes dans la polyarthrite rhumatoïde
… que les protéines adaptatrices Tyrosine Kinase Substrate 4 et 5 (Tks4,Tks5) sont essentielles à la formation de l’invadosome ainsi qu’à sa maturation menant à la dégradation de la matrice extracellulaire. L’objectif de cette étude est de déterminer si l’activation de Src chez les A-FLS et …
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Identification of motifs in the tribbles kinase required for substrate interactions
The gene tribbles encodes an atypical kinase that is evolutionarily conserved with important roles in regulation of cell signaling. In Drosophila melanogaster Tribbles (Trbl) directs the proteasomal degradation of the C/EBP transcription factor Slbo (slow border cells) to regulate cell migration in …
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3D Coiling at the Protrusion Tip: New Perspectives on How Cancer Cells Sense Their Fibrous Surroundings
… curvature sensing Insulin Receptor tyrosine kinase Substrate protein of 53 kDa (IRSp53) as a key regulator of protrusive activity with IRSp53 knockout (KO) cells exhibiting significantly slower protrusion dynamics and diminished coil width compared to their wild-type (WT) counterparts. We …
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Elucidating the mechanistic impact of single nucleotide variants in model organisms
… greatly aid our understanding of variant impact. Kinase–substrate phosphorylation is one of the most prominent post-translational modifications (PTMs) which is mediated by such motifs. We first describe a computational method which utilises interaction and phosphorylation data to predict sequence …
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Proteomic Analysis Delineates the Signaling Networks of Plasmodium falciparum
… Because the links between Plasmodium protein kinases as key cell cycle regulators to cellular events are largely unknown, it is of importance to define their cognate physiological substrates. To test the hypothesis that genetic screening would be a useful approach for discovery of candidate …
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THE ROLE OF ATP AND FK-506 BINDING PROTEIN IN THE COUPLED GATING OF SKELETAL RYANODINE RECEPTORS
… the indirect action of ATP to act as a kinase substrate or alter the cytoskeletal network. The addition of kinases, phosphatases and cytoskeletal modulators did not produce a significant disruption of coupled RyR1 gating. I also tested the role of addition of exogenous FKBP12 to RyR1s …
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Molecular Mechanisms Regulating Chemokine Receptor CXCR4 Signaling and Trafficking
… and hepatocyte growth factor-regulated tyrosine kinase substrate (HRS). It is the first complex that recognizes ubiquitinated CXCR4 and targets it for lysosomal degradation. We show that depletion of STAM-1 by siRNA and selective disruption of the STAM-1/arrestin-2 interaction accelerates agonist …
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