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Showing 1 to 20 of 22 for “"Kinase assay"”.
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Investigating the regulation of the PCTAIRE-1 protein kinase : a new target of AMP-activated protein kinase and beyond?
PCTK1 is a Cdc2-related protein kinase that is poorly characterised. Several studies have investigated its role and regulation, and have provided some insight into its upstream regulation by PKA/CDK5 phosphorylation. Proposed physiological functions include vesicle trafficking and neurite …
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Spatial Stochastic Modeling of the ErbB Receptor Family
… receptors are a family of 4 receptor tyrosine kinases that interact with one another through homo and heterodimer interactions. When these dimers form, the kinase domains on the receptor tails interact with one another, transphosphorylating one another, initiating a signal cascade. The …
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Enzymatic Characterization Of The Ammonia Tunnel In Helicobacter Pylori Asp-Trnaasn/glu-Trnagln Amidotransferase
… activities of <i>H. pylori</i> AdT (glutaminase, kinase and transamidase) and used these reactions as probes to examine the inter-domain communication and ammonia delivery mechanisms between this enzyme's two isolated active sites. We adapted and optimized an assay to kinetically characterize a …
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Growth regulation in the protozoan parasite Trypanosoma brucei: Evidence of tyrosine-specific protein kinase activity
… is conducted by a tyrosine-specific protein kinase. The activation of tyrosine kinases is an important step in transducing extracellular signals to a cell's interior. Until recently, protozoan organisms were thought not to possess tyrosine-specific protein kinases. This investigation …
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The Role of Post-Translation Regulation of Twist Expression In The Tumor Progression of Squamous Cell Carcinoma of Head and Neck
… has a putative phosphorylation site for casein kinase 2 (CK2) suggesting that this kinase could serve as a link between IL-6 stimulation and Twist stability. To test this hypothesis, we used a CK2 inhibitor and shRNA to CK2 and found that these interventions inhibited IL-6 stimulation of TWIST …
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Charakterisierung der konservierten Domänen des Transkriptionsfaktors N.t.BZI-1
… Zusammenhang untersucht werden. Durch einen Kinase-Assay sollen Phosphorylierungsereignisse als posttranslationale Modifikationen nach unterschiedlichen Stimuli nachgewiesen werden. Durch eine Grobkartierung der Phosphorylierungsstellen kann so möglicherweise eine Funktionszuweisung an die …
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
… 5 E. Immunoprecipitation and in vitro binding assay 5 F. In vitro kinase assay 6 G. Cell fractionation 6 H. Chromosome spreading 7 I. Immunocytochemistry 7 J. Live cell imaging 7 III. RESULTS 9 A. RSF1 localizes at mitotic kinetochores and directly interacts with PLK1 9 B. RSF1 plays a crucial …
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Up-modulation of Hepatocyte Growth Factor/ c-Met Signaling by Protein Kinase C-g and its Mechanism
Protein kinase C (PKC) family is composed of 11 isoenzymes, and has been known to down-modulate the tyrosine kinase activity of c-Met, the receptor for hepaotcyte growth factor (HGF). However, specified roles by PKC isoenzymes on the regulation of HGF/c-Met signaling have never been addressed yet. …
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Non-canonical sensing mechanisms in bacteria
… basis for bipolar pH sensing. Modified capillary assay was used to measure responses to opposite pH gradients. Through in vivo chimeric receptor and site-directed mutagenesis studies, we found that the lower regions of the extracellular ligand binding domains of the chemoreceptors are involved in …
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Development of Tyrosine Kinase Peptide Biosensors and Methods for Detection
<p>New methods to monitor tyrosine kinase activity are critical for studying kinases in cell biology, drug discovery and the clinic. Peptide-based biosensors for detection of kinase activity utilitize a kinase specific artificial peptide substrate, which can report intercellular kinase activity …
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Designing Compounds that Probe the Phosphatidylinositol 3-Kinase Signalosome
Phosphoinositide 3-kinases (PI3K) are major regulators of many cellular functions, and its hyperactivation is a major target for anticancer drug discovery. PI3Kα is the isoform most implicated in cancer, and this thesis is dedicated to investigating how specific protein-ligand interactions in the …
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Investigating the role of PINK1-dependent Rab signalling in Parkinson's disease
… phosphorylated across various sites. Large-scale kinase assay screens were employed to identify the direct upstream Rab kinase. Although the Ser111 kinase remained elusive, further novel Thr72 Rab kinases were identified. <br/><br/>In order to elucidate the function of Rab Ser111 phosphorylation, …
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Anti-cancer Functions and Mechanisms of a pRb2/p130 Peptide Fragment
… pRb2/p130 was reported to be able to inhibit the kinase activity of Cdk2. The region responsible for the inhibitory effect was further narrowed down to a 39-amino-acid sequence, which was named as Spa310. In this dissertation, the anti-cancer functions and mechanisms of Spa310 were studied. The …
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Characterization of Jak, Stat, and Src Interactions In Head and Neck Squamous Cell Carcinoma
… During short-term Src inhibition, Janus kinase (Jak) 2, and signal transducer and activator of transcription (STAT) 3 and STAT5 are dephosphorylated and inactivated. Following sustained Src inhibition, STAT5 remains inactive, but Jak2 and STAT3 are reactivated following their early …
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Isolation and Functional Mapping of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein DNA-Damage Complexes
… itself. We identified the DNA-dependent Protein Kinase catalytic subunit (DNA-PKcs) as a novel Tax-interacting protein. Using bioinformatics analysis, we created a database of Tax-interacting proteins and, using this tool, identified DNA-PKcs as a predicted member of the Tax complex.</p> …
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
Pik1 is a yeast phosphatidylinositol-4 kinase that is critical for vesicular traffic to the plasma membrane and endosomes from the trans-Golgi (1, 2). Pik1 is a soluble enzyme, and the small myristoylated, Ca2+ binding, EF hand protein, Frequenin (Frq1) facilitates its membrane localization (3). It …
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Novel peptide-based approaches to study the activity and substrate specificity of protein kinases
Protein phosphorylation, catalyzed by protein kinases, is the predominant post-translational modification made to proteins. This process is readily reversible, with protein phosphatases catalyzing removal of the installed phosphate groups. Together, protein kinases and phosphatases modulate the …
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Mechanistic Investigation of Genotype-Phenotype Correlations in PIK3R1-Related Diseases
… subunits of Class IA phosphoinositide 3-kinases (PI3Ks). These regulatory subunits heterodimerise with one of three catalytic subunit isoforms, namely p110$\alpha$, p110$\beta$, or p110$\delta$. Class IA PI3Ks are critical enzymes involved in fundamental metabolic and mitogenic signalling …
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Transcriptional and Translational Products of Avian Erythroblastosis and Avian Myeloblastosis Viruses
… proteins, AEV p75 was not found to be a protein kinase using the immune complex protein kinase assay. AEV nonproducer cells were found to contain two viral related RNA species of 28 S and 21 S. Both RNA species contain the AEV unique sequences and the "strong stop" region of the viral genome, as …
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Analysis of cyclin dependent kinases in Leishmania
… to further explore the role of cyclin dependent kinases and cyclins in the protozoan parasite Leishmania major. Cdks in kinetoplastids, CRKs, are the key regulators that allow cells to progress through different cell cycle phases and promote parasite proliferation during infection. In chapter 3 …
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