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Showing 1 to 3 of 3 for “"Kemp eliminase"”.
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
… important esterase reaction and another for the Kemp elimination, an artificial reaction. In Chapter 2, I describe the state-of-the-art fluorescence-activated droplet sorter (FADS) I developed for use with fluorogenic enzyme substrates. I improved this instrument incrementally based on the needs …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… systems were investigated: the de novo designed Kemp Eliminase (KE07), the metallo-beta-lactamases NDM1 and VIM2, and the N-acyl-homoserine lactonase AiiA. Based on these studies, I was able to identify three clear phenomena that are important in molecular evolution: first, preorganization of the …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… systems were investigated: the de novo designed Kemp Eliminase (KE07), the metallo-beta-lactamases NDM1 and VIM2, and the N-acyl-homoserine lactonase AiiA. Based on these studies, I was able to identify three clear phenomena that are important in molecular evolution: first, preorganization of the …