Global ETD Search
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Showing 1 to 5 of 5 for “"KPf"”.
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Development of high-throughput screening assays to identify small molecule inhibitors of Plasmodium falciparum Hsp70-1
… the positive control, while those expressing KPf-V436F were the negative control and the cells heterologously expressing KPf were the test cells. KPf contains a substrate binding domain (SBD) from PfHsp70-1. Protein expression was confirmed by sodium dodecyl sulfate-polyacyrlyamide and Western …
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Comparative analysis of a chimeric Hsp70 of E. coli and Plasmodium falciparum origin relative to its wild type forms
… system. To this end, a chimeric Hsp70, KPf consisting of E. coli DnaK NBD and P. falciparum Hsp70-1 (PfHsp70-1) SBD was previously designed. KPf was shown to confer cytoprotection to E. coli DnaK deficient cells that were subjected to heat stress. In this study it was proposed that KPf …
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Sedimentology of Kingston Peak Formation KP1 Beds in Beck Canyon Region, Kingston Range, California
<p>The Neoproterozoic Kingston Peak Formation (KPF) is known for its diamictites interpreted as of glacial origin. The KPF overlies the Beck Spring Dolomite (BSD) that contains microfossils and microbialites. This study aims to test correlation between outcrops and understand the abrupt change from …
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In cellulo and biophysical exploration of the role of GGMP residues of Hsp70
… using E. coli Hsp70 (DnaK) and chimeric protein, KPf as models. Chimeric protein KPf is made up of NBD of DnaK and SBD of PfHsp70-1. E. coli DnaK lacks the GGMP residues that are present in PfHsp70-1. DnaK-G (DnaK modified to include GGMP residues) was created to elucidate the function of these …
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Effects of co-expression of chaperone combinations on production of soluble plasmodial protein PfAdoMetDC in E. coli
… PfHsp70 with DnaJ; combination 3 (pBB535-KPfJ) encoding KPf with DnaJ; combination 4 (pBB542) encoding DnaK with DnaJ and GroEL; combination 5 (pBB542-Pf70JE) encoding PfHsp70 with DnaJ and GroEL; and combination 6 (pBB542-KPfJE) encoding KPf with DnaJ and GroEL. The effects of these …