Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"KATP-channel"”.
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Investigation of KATP channel function in response to metabolic and pharmacological manipulation, in the hypothalamic GT1-7 cell line
… and a reduction in the sensitivity of the KATP channel to activation by MgADP. In an attempt to reverse the changes observed in KATP channel activity, the SUR1-selective KATP channel opener, NN414, was applied chronically to GT1-7 cells. However, chronic KATP channel activation severely …
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K(ATP) channel Kir62 subunit distribution differs between muscles and between fiber types in skeletal muscle
The activity of the ATP-sensitive potassium (KATP) channel, whose sensitivity to ATP and ability to permit K+ flux together allow the channel to couple the metabolic state of a cell to its membrane excitability, is important in several tissues for the maintenance of glucose homeostasis and …
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Spontaneous tone in deoxycorticosterone acetate-salt hypertensive rats
… mediated by its COX metabolites. Cromakalim, a KATP channel opener, abolished spontaneous tone in a glibenclamide-sensitive fashion. In aortic cells from DOCA-salt hypertensive rats, ATP-dependent K+(KATP) channel currents were either absent or weak in response to challenges by elevated …
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Zebrafish Models of KATP Channelopathies
… and function of ATP-sensitive potassium (KATP) channels in mammalian tissues, as well as the molecular basis of the channelopathies that result from loss-of-function or gain-of-function mutations in these channels. These studies have also highlighted that the available pharmacology to …
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Investigations into the roles of potassium channels in hair growth. Studies confirming the presence of several ATP-sensitive potassium (K+ATP) channels in hair follicles and exploring their mechanism of action using molecular biological, cell culture, organ culture and proteomic approaches.
… loss is minoxidil, an ATP-sensitive potassium (KATP) channel opener whose mechanism of stimulation is unclear. The regulatory component of KATP channels has three forms: SUR1, SUR2A and SUR2B which all respond to different molecules. Minoxidil only opens SUR2B channels, though SUR1 and SUR2B are …
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The role of ATP-sensitive inwardly rectifying potassium channels in the honey bee (Apis mellifera L.)
… for inwardly-rectifying ATP-sensitive potassium (KATP) channels in the cardiac regulation of the fruit fly antiviral immune response, but no information is available on their role in the heart-specific regulation of bee immunity. The results of this work demonstrate that KATP channel modulators …
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Mechanistic investigation of coordinated conformational changes in multisubunit ion channels and enzymes
Many enzymes and ion channels consist of multiple subunits and/or multiple distinct functional components. Coordinated conformational changes through allosteric interactions between subunits and/or between functional units can efficiently regulate protein activity. This dissertation describes …
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Recurrent Hypoglycaemia-examining Impact and Strategy
… finding that hypothalamic ATP-sensitive K + (KATP) channels are integral to the detection of<br/>hypoglycaemia as well as instrumental to mounting a counter-regulatory response to hypoglycaemia. In a randomised, double-blind hyperinsulinaemic hypoglycaemia clamp<br/>study, I compared the …
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Role of KATP channels in angiogenesis
… on the activation of ATP-sensitive potassium channels (KATP). Intriguingly, C-type Natriuretic Peptide (CNP), which is also known to activate KATP, has been reported to promote endothelial cell growth; however, its angiogenic properties have not been explored at any depth. This pattern …
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Type-5 Phosphodiesterase Inhibition in the Prevention of Doxorubicin Cardiomyopathy
… protecting against apoptosis via mitochondrial KATP channel opening in vitro. The current investigations are designed to determine if sildenafil, a phosphodiesterase-5 inhibitor and known mitochondrial KATP channel opener, would protect against chronic doxorubicin cardiomyopathy both in vivo and …
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The role of the large conductance Ca2+-activated K+ channel in adenosine receptor-mediated cytoprotection
… and trigger effectors such as the mitochondrial KATP channel – however, the role of the mitochondrial BKCa channel in adenosine receptor-mediated cardioprotection has not been investigated. GPCR assays provided evidence for functional expression of Gi-coupled adenosine A1 and κ-opioid receptors, …
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Osteoarthritis: the role and mechanisms of the chondroprotective peptide Urocortin I
… Kir6.1, Kir6.2, SUR1 and SUR2B subunits of the KATP channels suggesting that functional KATP channels are also present in these cells, which represent a further putative mechanism of action for UCNI. Quantitative PCR studies were performed to determine regulation of CRFR and Kir6.1 expression by …
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Photochemical harpoons: covalent labels for multi-protein complexes
… between the toxin analogue and the enzymes. The KATP channel opener, diazoxide displays marked cardioprotective effects and is reported to bind to mitochondrial KATP channels. However, the molecular structure of these channels is still largely unknown.
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Extracellular adenosine as a novel neuroimmune modulator
… (KO) mice. Co-perfusion of adenosine with the KATP channel blocker, glyburide, also blocked adenosine-dependent activation of caspase-1. Finally, mice i.p. injected with 50 μm adenosine displayed reduced locomotor activity and food intake and increase anxiety-like bahviors. These …
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Exploration of Hypotensive Effects of Novel Hydrogen Sulfide Donors: Mechanisms and Their Delivery
… H2S synthesis, and glibenclamide (GLBN), a KATP channel blocker. These studies confirmed that both endogenous H2S production and the activation of KATP channels are crucial in mediating the observed effects, thereby supporting the biological significance of the donor’s design. To establish a …
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Membrane trafficking of ATP-sensitive potassium channels
ATP-sensitive potassium (KATp) channels are known to play a vital role in the regulation of insulin secretion from pancreatic 0-cells. Changes in the ratio of [ATP]/[ADP] within the cell are known to regulate the activity of channels, but very little is known how the number of channels at the cell …
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Regulation of glucagon secretion from pancreatic alpha cells
… excitable and equipped with different ion channels. But unlike β-cells, α-cells electrical activity is only possible within a narrow window of low K(ATP) channel activity. At low glucose, the K(ATP) channels are almost fully – but not completely –inhibited. This allows firing of action …
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Mechanisms of pathological adipogenesis
… including some forms of osteoporosis. Ion channels and ghrelin signalling have been shown to be involved in regulating many cell processes such as proliferation, differentiation and apoptosis, particularly in bone. This thesis investigated the roles of ghrelin and ion channels in the …
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Studies of TNF- α-induced -GSIS Inhibition in INS-1 β-cell
… treatment for 24 hours. Bay K8644, Ca(2+)+ channel agonist, induced insulin secretion decreased about 50% by pretreatment of TNF-α in INS-1 cell. But KCl-induced insulin serection, evocked by depolarization, was decreased a little. And PMA, forskolin induced insulin secretion was not …
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Structural Study of Disease Relevant ABC Transporters-Cystic Fibrosis Transmembrane Conductance Regulator and ABCA4
… exceptions: SUR—a regulatory protein for the KATP channel, cystic fibrosis transmembrane conductance regulator (CFTR)—an chloride channel and ABCA4 (aka the Rim protein and ABCR)—an retinylidene-PE importer. In addition to their unique functional properties, both CFTR and ABCA4 are very …
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