Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"K562 CELLS"”.
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The role of signal regulatory protein alpha in regulating macrophage subset activity in homeostasis and inflammation
… a co-culture system between CD47+ HL-60 and K562 cells, and SIRPα+ Mφ subsets in order to investigate whether the regulatory effect of SIRPα-CD47 ligation extends to negative regulation of TLR-mediated TNFα production, and how this differs between Mφ subsets and under various TNFα-inducing …
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Avaliação do potencial citotóxico dos compostos organoselenados, HSe-01 e HSe-02.
… in terms of removal and attack of cancer cells, especially early in the disease. However, those treatments haven’t been perfectly selective. Consequently, the study of new selective target compounds with therapeutic potential against cancer has been intensified. Selenides compounds are one …
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Identification of a Minimal Cis-element and Cognate Trans-factors Required for the Regulation of Rac2 Gene Expression during K562 Cell Differentiation
… for the induction of Rac2 gene expression during K562 cell differentiation. The Rho family GTPase Rac2 is expressed in hematopoietic cell lineages and is further up-regulated upon terminal myeloid cell differentiation. Rac2 plays an important role in many hematopoietic cellular functions, such as …
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Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection
… HIV-specific ADCC responses. To do this, NK cells from a panel of healthy HIV-negative individuals were screened for their ability to directly kill the tumour cell line K562, as a measure of direct NK cell cytotoxicity. The individual with the highest granzyme B and caspase activity against …
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β-Globin Enhancers Regulate Bistable Gene Expression States
… this model has been challenging to test in human cells. To examine the role of the human β-globin enhancers in controlling single-cell expression phenotypes, we engineered a haploid locus in K562 cells that fluorescently reports the expression of HBG1. We isolated combinatorial enhancer deletion …
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Development of novel isolation methodologies for microvesicles and exosomes, as potential biomarkers for health (Therapeutic Lifestyle Changes), and use of microvesicle-delivered β-Gly in erythroleukaemia
… shown to inhibit MV release. MV release from K562 cells was inhibited by Y27632 and calpeptin but less effectively by chlorpromazine or methyl-β-cyclodextrin, MV release from THP-1 acute monocytic leukaemia cells was greater than from peripheral blood monocytes which were in turn shown to …
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Mapping and characterisation of genomic binding sites of the chromatin barrier protein VEZF1
… human genome, known as the ENCODE regions, in K562 erythroid cells. It was found that VEZF1 preferentially targets gene regulatory elements and promoters of actively transcribed genes in particular. A significant proportion of intergenic sites were identified at the boundary regions between …
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Studies of the interaction of hepatitis B virus with human haemopoietic cells.
… of hepatitis B virus (HBV) with haemopoietic cells and cell lines, to address the reported tropism of HBV for haemopoietic tissues. Emphasis was directed at demonstrating specific attachment of HBV to defined subpopulations of peripheral blood leucocytes (PBL) and bone marrow cells (BM), and …
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Investigating the anti-proliferativeproperties and their mechanism(s) of actionof marine derived glycosaminoglycans fromtwo marine sources
… differences between the two derived GAGs on K562 cancer cells. Furthermore, the MTT assay was employed to investigate the chemotherapeutic properties of the prawn derived GAGs on healthy and cancer cell lines other than K562. The final aspect of this investigation was using affinity …
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Avaliação do potencial antileucêmico de adutos aromáticos de morita-baylis-hillman.
… (MBHA), using the culture of some leukemic cells, HL - 60, K562, K562 - Lucena and MOLT - 4, as well as normal cells from peripheral blood mononuclear cell (PBMC). The effect of these MBHA synthetic molecules were evaluated by MTT reduction and neutral red uptake techniques and the mechanism …
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Functional Investigation of Dual αvβ3 and αllbβ3 Integrin Inhibition in Haematological and Solid Tumour Models
… in inducible and natural expression models (K562 and MCF-7 cells respectively), functional tests for cellular adhesion, detachment and migration were determined. Phorbol 12-myristate 13-acetate (PMA)-treated K562 cells showed increased adhesion on fibrinogen compared to untreated cells. …
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Elucidating the role of Mitoferrin (Mfrn), Iron Regulatory Proteins (IRP1 and IRP2) and Hephaestin (Heph) in Iron Metabolism by tagSNP and Protein-Protein Interaction (PPI) analysis
… Hephaestin (Heph) facilitate iron release from cells is poorly understood. The work in this thesis tried to establish the role of different iron metabolic proteins, Mitoferrin (Mfrn), IRPs and Heph in iron homeostasis. Analysis of 18 tagSNPs in the Mfrn gene was carried out in an Asian-Caucasian …
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Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death
… during hibernation, we and others have seen that cells from hibernating rodents cultured in vitro maintain this ability. Although others have studied characteristics of cells from hibernating and non-hibernating organisms, the genes and pathways that are involved in cold-induced cell death have …
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Circulating Natural Killer Cells and Natural Killer Receptor Positive T Cells In Patients with Hepatitis C
… chronic liver disease. Natural killer (NK) cells are thought to mediate immunity against viruses. They can lyse virus infected cells and release anti-viral cytokines. The roles of NK cells and natural killer receptor positive (NKR+) T cells in immunity agamst HCV is largely unknown. The aim …
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Modulation of heme oxygenase-1 activity by novel synthetic compounds for pharmacological applications
… may be detrimental, especially in cancer cells where the enzyme can sustain tumor aggressiveness and resistance to therapies. Therefore, HO-1 inhibition has been proposed as a stand-alone or adjuvant anticancer therapy, and a library of imidazole-based HO-1 inhibitors has been synthesized. …
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Role of Stat3 In Human Nk Cell Functions
<p>Natural Killer (NK) cells are cytotoxic lymphocytes, which play a critical role in the immune response against malignant cells and microbial infections. NK cells are equipped with activating receptors, which upon detecting ligands expressed on stressed cells induce cytolytic activity of NK …
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Redirecting T Cells With Chimeric Antigen Receptors to Target Cd123+ Leukemia
… g><strong>I</strong><strong>N</strong><strong>G T CELLS WITH CHIMERIC ANTIGEN RECEPTORS TO TARGET CD123</strong><strong>+ </strong><strong>LE</strong><strong>U</strong><strong>K</strong><strong>E</strong><strong>M</strong><strong>I</strong><strong>A</strong></h1> <p>Radhika Thokala, Ph.D*</p> …
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Repurposing Alzheimer’s disease medications in a drug combination for the study of the crosstalk between cell apoptosis and autophagy in the modulation of cell death in chronic myeloid leukaemia
… (cell model of AD) and K-562 (cell model of CML) cells were individually treated with the test drugs for 24 and 48 h, depending on the assay. Cell viability was assessed by overlaying the treated cells with CyQUANT® Direct assay dye for 40 min before fluorescence signal detection at a wavelength …
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ΜΕΛΕΤΗ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΟΥ ΑΝΘΡΩΠΙΝΟΥ ΕΜΒΡΥΟΝΙΚΟΥ Ε-ΣΦΑΙΡΙΝΙΚΟΥ ΓΟΝΙΔΙΟΥ
… THE HUMAN HYBRID PUTKO CELL LINE AND FROM HELA CELLS TRANSIENTLY TRANSFECTED WITH THE E-GLOBIN GENE. FOR HELA CELL TRANSIENT EXPERIMENTS WE CONSTRUCTED A SERIES OF PLASMID VECTORS CONTAINING THE E-GLOBIN GENE AND, IN ALL POSSIBLE COMBINATIONS, THE SV40 ENHANCER SEQUENCES AND SV40 ORIGIN …