Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 66 for “"K562"”.
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Análise funcional do gene FOXO3 na indução de hemoglobina fetal em células K562
As β-hemoglobinopatias são conhecidas como desordens genéticas com ampla distribuição mundial e prognóstico clínico crônico e variável. Essas patologias têm seus sintomas e sequelas amenizados com o aumento dos níveis de hemoglobina fetal (HbF; α2γ2). O único fármaco autorizado pelo Food and Drug …
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COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
… cell line and chronic myeloid leukemia model, K562. Both knockdowns had similar consequences for CRL activity whilst having divergent effects on the levels of SRS mRNA. Knockdown of either subunit also resulted in a common sequential proteasome-dependent loss of SRS proteins, an observation …
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Identification of a Minimal Cis-element and Cognate Trans-factors Required for the Regulation of Rac2 Gene Expression during K562 Cell Differentiation
… for the induction of Rac2 gene expression during K562 cell differentiation. The Rho family GTPase Rac2 is expressed in hematopoietic cell lineages and is further up-regulated upon terminal myeloid cell differentiation. Rac2 plays an important role in many hematopoietic cellular functions, such as …
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Der Einfluss von HLA-G und HLA-E auf Large Granular Lymphocytes
… HLA-E), zum anderen die Leukämiezellllinien K562 (untransfiziert), K562 (mit HLA-G transfiziert) und K562 (mit HLA-E transfiziert) als Modell für fetalen Trophoblasten verwendet. In Kokulturen dieser Zelllinien mit Large Granular Lymphocytes (LGL, CD 56++) sowie LGL freier Dezidua (WASH) …
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Avaliação do potencial citotóxico dos compostos organoselenados, HSe-01 e HSe-02.
… cytotoxic effect on human cancer lines (HL-60, K562, K562-Lucena, and Thp-1) and a less expressive cytotoxic effect on non-cancerous cells (HUVEC), from a concentration of 6.25 μM. Due to potent and selectivity cytotoxic effect of the compounds in 24-hour in K562 cells line, it was evaluated the …
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Regulation der Genexpression bei der Erythroiden Differenzierung
… anderen Modelle basieren auf der CML-Zelllinie K562, wobei die Zellen mit AraC, Natriumbutyrat oder Hämin behandelt wurden. Bei allen Modellen wurden Zellproben in Form von Zeitreihen entnommen. Mit Hilfe der aus den Zellen präparierten total-RNA wurden Hybridisierungsproben hergestellt und auf …
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Avaliação do potencial antileucêmico de adutos aromáticos de morita-baylis-hillman.
… the culture of some leukemic cells, HL - 60, K562, K562 - Lucena and MOLT - 4, as well as normal cells from peripheral blood mononuclear cell (PBMC). The effect of these MBHA synthetic molecules were evaluated by MTT reduction and neutral red uptake techniques and the mechanism of cell death …
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Functional Investigation of Dual αvβ3 and αllbβ3 Integrin Inhibition in Haematological and Solid Tumour Models
… in inducible and natural expression models (K562 and MCF-7 cells respectively), functional tests for cellular adhesion, detachment and migration were determined. Phorbol 12-myristate 13-acetate (PMA)-treated K562 cells showed increased adhesion on fibrinogen compared to untreated cells. …
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The role of signal regulatory protein alpha in regulating macrophage subset activity in homeostasis and inflammation
… a co-culture system between CD47+ HL-60 and K562 cells, and SIRPα+ Mφ subsets in order to investigate whether the regulatory effect of SIRPα-CD47 ligation extends to negative regulation of TLR-mediated TNFα production, and how this differs between Mφ subsets and under various TNFα-inducing …
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Predicting DNA Methylation State of CpG Dinucleotide Using Genome Topological Features and Deep Networks
… data from immortalised myelogenous leukemia (K562) and healthy lymphoblastoid (GM12878) cell lines to train the learning models and assess prediction performance. We have tested various SdA architectures with different configurations of hidden layer(s) and amount of pre-training data and …
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NLRP2 expressziójának vizsgálata különböző immortalizált sejtvonalakon
… humán sejtvonalakon. A kísérletek során THP-1, K562, Caco2, A549, NB4, U937, Hela és MCF-7 sejtvonalakat használtunk, melyeket 6, valamint 24 órás GM-CSF és dexametazon kezeléseknek vetettük alá. A kezelt sejtekből RNS-t izoláltunk, majd RT-qPCR segítségével meghatároztuk az NLRP2 relatív …
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Circulating Natural Killer Cells and Natural Killer Receptor Positive T Cells In Patients with Hepatitis C
… and interleukin-2-activated killing o f K562 and Daudi cells by peripheral blood mononuclear cells (PBMCs) were assessed using 5'chromium release assays. NK cells and NKR+ T cells were depleted in patients with chronic HCV compared to the healthy controls (3.4% vs 13.7% for NK cells; …
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Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection
… ability to directly kill the tumour cell line K562, as a measure of direct NK cell cytotoxicity. The individual with the highest granzyme B and caspase activity against K562 cells was chosen as the universal NK cell donor for this study.
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Investigating the anti-proliferativeproperties and their mechanism(s) of actionof marine derived glycosaminoglycans fromtwo marine sources
… differences between the two derived GAGs on K562 cancer cells. Furthermore, the MTT assay was employed to investigate the chemotherapeutic properties of the prawn derived GAGs on healthy and cancer cell lines other than K562. The final aspect of this investigation was using affinity …
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Avaliação da citotoxicidade e da indução de apoptose de adutos da reação Morita-Baylis-Hillman derivados da isatina em células tumorais
… linhagens tumorais (MCF-7, HEP-2, HepG2, HL60, K562 e NCI H292), nos quais os 8 mais promissores foram testados em células mononucleares do sangue periférico (CMSP). As CI50s determinadas pelo método do MTT após 72h para células tumorais dos adutos mais citotóxicos variaram entre 0,044uM e …
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Opto-Electronic Modulation of Quantum Dots by Cells and Bioactive Molecules
… of optically excited colloidal QDs with HL60, K562, and T98G cell lines are performed. Our results show statistically significant (p < 0.001 and p < 0.0001) quenching of the emission spectra of the colloidal dispersions due to bioactive molecules in common chemotherapeutic drugs and due to …
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Membrane Bound Il21 Promotes Natural Killer Cell Expansion Through Mir 124-3P Mediated Regulation
… for ex vivo expansion of human NK cells using K562 expressing membrane bound IL-21 (K562.mbIL21), and showed that K562.mbIL21 enables significantly higher expansion of functionally potent NK cells compared to K562.mbIL15. Both membrane bound IL15 (mbIL15) and membrane bound IL21 (mbIL21) …
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Studies of the interaction of hepatitis B virus with human haemopoietic cells.
… binding of HBV to the erythroleukaemia cell line K562 was observed, while other myeloid cell lines did not appear to bind virus. This assay was then adapted to a suspension cell assay with analysis by flow cytometry, using phycoerythrin as the detecting fluorochrome. The parameters of binding were …
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Mapping and characterisation of genomic binding sites of the chromatin barrier protein VEZF1
… human genome, known as the ENCODE regions, in K562 erythroid cells. It was found that VEZF1 preferentially targets gene regulatory elements and promoters of actively transcribed genes in particular. A significant proportion of intergenic sites were identified at the boundary regions between …
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Differenzierungsabhängige Modulation der Zytokin- und Chemokinexpression bei humanen myeloischen Zell-Linien
… von drei myeloischen Zell-Linien (NB4, HL60, K562) untersucht, ob die Zyto- und Chemokinexpression durch eine Differenzierung moduliert wird. Markerzytokine für bestimmte Richtungen der Differenzierung sollten identifiziert und <br>linienspezifische Zytokin- und Chemokinprofile bestimmt …
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