Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Jurkat T cells"”.
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Regulation Mechanisms of TIM-3 expression in Jurkat T cells and HMC-1 cells
… TIM-3의 발현 조절 기작을 밝히기 위해 다양한 inhibitor들을 사용한 결과 Jurkat T 세포와 인간 CD4+ T 세포에서는 MEK 경로, HMC-1 세포에서는 MEK 경로와 SMAD 의존적 경로가 TIM-3 발현에 중요하게 작용함을 밝혔다. 또한 T 세포에서 TIM-3 발현 조절에 중요하게 작용하는 promoter 부분을 찾기 위해 다양한 길이의 TIM-3 promoter를 포함하는 luciferase reporter vector를 제작하여 luciferase 활성도를 측정하였다. 그 결과 TIM-3 …
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Regulation and function of hyaluronan binding by CD44 in the immune system
… fusion protein expressed in HEK293 cells, and full-length CD44 expressed in murine L fibroblast cells. In bone marrow-derived macrophages, hyaluronan binding induced by the inflammatory cytokines tumor necrosis factor-α and interferon-γ corresponded with reduced chondroitin sulfate …
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Upstream conserved sequence element that regulates TIM-3 induction in CD4+ T cell
… of TIM-3 transcription in human CD4+ T cells between -70 kbp to -1 bp from the TIM-3 transcription start site. Given that regulatory DNA regions show nucleotide sequence homology between species, four conserved non-coding sequences (CNS -29, CNS -53, CNS -56 and CNS -63) were analyzed by …
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Development of a Lentivirus-mediated Gene Therapy Targeting HIV-1 RNA to Eliminate Infected Cells
… therapeutic strategy that aims to eliminate cells actively expressing HIV-1 and those harbouring HIV-1 reactivated from latency. This approach hijacks the currently unexploited HIV-1 alternative RNA splicing process to functionalize a defective cell suicide enzyme (*HSVtk*<sub>ΔAUG1</sub>) …
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Structure-based development of novel chemical scaffolds as inhibitors of the store-operated calcium entry pathway
… using Fura-2 based Ca2+ imaging in RBL1-cells identified 5 potent molecules (namely compounds E1, E7, E8, E10, E11) with consistent Thapsigargin (Tg)-evoked SOCE inhibitory properties. I confirmed this by assessing the effect of the compounds on nuclear translocation of NFAT (Nuclear …
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Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… positive controls. Three different cell lines, Jurkat T cells, CaCo-2 cells and HepG-2 cells, representing the main target organs, were exposed to OA and AZA1-3 at different concentrations and exposure times. Data obtained from the COMET assay showed an increase in DNA fragmentation for all …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… splicing of apoptotic genes. To test this, Jurkat T cells were treated with the selective inhibitor of iPLA2β, bromoenol lactone (BEL), RNA was isolated and converted to cDNA, and caspase 9 and BCL-x mRNA viii species were amplified using RT-PCR. Inhibition of iPLA2β activity with BEL caused …
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Untersuchungen zur Rolle von Zytokinen bei der Beta-2-Mikroglobulin-Synthese in verschiedenen humanen Zelltypen
… of beta-2-M gene expression in human hepatoma cells, T-lymphocytes and monocytes. Methods. HepG2 and HuH7 human hepatoma cells, Jurkat T-cells, monocytic MonoMac6 cells, primary human monocytes and synoviocytes were stimulated with IL-1-beta, IL-6, interferon-alpha (IFN-alpha), IFN-gamma or …
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The Suppressive Mechanisms of TIM3 on T Cell Cytokine Production and The Anti-Tumor Effect of TIM3-Pathway Inhibition
… (TIM3) has been shown to be expressed on Th1 cells and negatively regulates these cell functions. TIM3 has been implicated in the Th1-driven pathogenesis of autoimmune disease and the viral infection. However, molecular mechanisms of TIM3 inhibiting Th1 cells and the efficacy of tumor vaccine …
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Tim-3 and Cell Death in Murine Mercury Induced Autoimmunity
… induces both polyclonal activation of B cells, as well as the production of highly specific autoantibodies targeting the nucleolar protein fibrillarin. Tim proteins are a family of immune cell surface molecules that have been implicated in modulation of autoimmune diseases in both humans …
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The role of the adapter protein TSAd in T cell activation
Introduction: T cells are a part of the adaptive immune system, which is designed to attack pathogens in the body. T cells recognize antigen presented by HLA molecules on the surface of antigen presenting cells (APC) through their T cell receptor. Conjugation formation between T cells and APC turns …
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Autogramin-2, a synthetic sterol, inhibits T cell adhesion and effector function
… boosts anti-viral and anti-cancer immunity in T cells. Inhibition of cholesterol transport from the plasma membrane to the endoplasmic reticulum in adoptively transferred T cells therefore constitutes a promising therapeutical avenue for improving cancer therapy. I hypothesised that inhibition of …
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Developing an in vitro model to study trained immunity
… ex vivo using human peripheral blood mononuclear cells (PBMCs) and bone marrow derived macrophages (BMDMs) from mice. However, we aim to develop and characterize an in vitro cell model to study trained immunity. J774A.1 macrophages and THP-1 monocytes were evaluated as murine and human models, …
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Investigating The Distinct Role of Pd-L2 In Modulating Human T Cells Responses
… the PD-1/PD-L1/PD-L2 signaling axis in human T cells. To this end, we established an in vitro experimental system using human Jurkat T cells that allowed us to study the T cell regulatory functions of PD-1/PD-L1 and PD-1/PD-L2 complexes, exclusive of other protein-protein interactions on the …