Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Jurkat T cells"”.
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Regulation Mechanisms of TIM-3 expression in Jurkat T cells and HMC-1 cells
… TIM-3의 발현 조절 기작을 밝히기 위해 다양한 inhibitor들을 사용한 결과 Jurkat T 세포와 인간 CD4+ T 세포에서는 MEK 경로, HMC-1 세포에서는 MEK 경로와 SMAD 의존적 경로가 TIM-3 발현에 중요하게 작용함을 밝혔다. 또한 T 세포에서 TIM-3 발현 조절에 중요하게 작용하는 promoter 부분을 찾기 위해 다양한 길이의 TIM-3 promoter를 포함하는 luciferase reporter vector를 제작하여 luciferase 활성도를 측정하였다. 그 결과 TIM-3 …
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Regulation and function of hyaluronan binding by CD44 in the immune system
… fusion protein expressed in HEK293 cells, and full-length CD44 expressed in murine L fibroblast cells. In bone marrow-derived macrophages, hyaluronan binding induced by the inflammatory cytokines tumor necrosis factor-α and interferon-γ corresponded with reduced chondroitin sulfate …
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Upstream conserved sequence element that regulates TIM-3 induction in CD4+ T cell
… of TIM-3 transcription in human CD4+ T cells between -70 kbp to -1 bp from the TIM-3 transcription start site. Given that regulatory DNA regions show nucleotide sequence homology between species, four conserved non-coding sequences (CNS -29, CNS -53, CNS -56 and CNS -63) were analyzed by …
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Development of a Lentivirus-mediated Gene Therapy Targeting HIV-1 RNA to Eliminate Infected Cells
… therapeutic strategy that aims to eliminate cells actively expressing HIV-1 and those harbouring HIV-1 reactivated from latency. This approach hijacks the currently unexploited HIV-1 alternative RNA splicing process to functionalize a defective cell suicide enzyme (*HSVtk*<sub>ΔAUG1</sub>) …
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Structure-based development of novel chemical scaffolds as inhibitors of the store-operated calcium entry pathway
… using Fura-2 based Ca2+ imaging in RBL1-cells identified 5 potent molecules (namely compounds E1, E7, E8, E10, E11) with consistent Thapsigargin (Tg)-evoked SOCE inhibitory properties. I confirmed this by assessing the effect of the compounds on nuclear translocation of NFAT (Nuclear …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… splicing of apoptotic genes. To test this, Jurkat T cells were treated with the selective inhibitor of iPLA2β, bromoenol lactone (BEL), RNA was isolated and converted to cDNA, and caspase 9 and BCL-x mRNA viii species were amplified using RT-PCR. Inhibition of iPLA2β activity with BEL caused …
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Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… positive controls. Three different cell lines, Jurkat T cells, CaCo-2 cells and HepG-2 cells, representing the main target organs, were exposed to OA and AZA1-3 at different concentrations and exposure times. Data obtained from the COMET assay showed an increase in DNA fragmentation for all …
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The Suppressive Mechanisms of TIM3 on T Cell Cytokine Production and The Anti-Tumor Effect of TIM3-Pathway Inhibition
… (TIM3) has been shown to be expressed on Th1 cells and negatively regulates these cell functions. TIM3 has been implicated in the Th1-driven pathogenesis of autoimmune disease and the viral infection. However, molecular mechanisms of TIM3 inhibiting Th1 cells and the efficacy of tumor vaccine …
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Tim-3 and Cell Death in Murine Mercury Induced Autoimmunity
… induces both polyclonal activation of B cells, as well as the production of highly specific autoantibodies targeting the nucleolar protein fibrillarin. Tim proteins are a family of immune cell surface molecules that have been implicated in modulation of autoimmune diseases in both humans …
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The role of the adapter protein TSAd in T cell activation
Introduction: T cells are a part of the adaptive immune system, which is designed to attack pathogens in the body. T cells recognize antigen presented by HLA molecules on the surface of antigen presenting cells (APC) through their T cell receptor. Conjugation formation between T cells and APC turns …
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Autogramin-2, a synthetic sterol, inhibits T cell adhesion and effector function
… boosts anti-viral and anti-cancer immunity in T cells. Inhibition of cholesterol transport from the plasma membrane to the endoplasmic reticulum in adoptively transferred T cells therefore constitutes a promising therapeutical avenue for improving cancer therapy. I hypothesised that inhibition of …
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Investigating The Distinct Role of Pd-L2 In Modulating Human T Cells Responses
… the PD-1/PD-L1/PD-L2 signaling axis in human T cells. To this end, we established an in vitro experimental system using human Jurkat T cells that allowed us to study the T cell regulatory functions of PD-1/PD-L1 and PD-1/PD-L2 complexes, exclusive of other protein-protein interactions on the …