Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"Jurkat Cells"”.
-
Investigation of the effects of chinese herb medicine on TNF-[alpha] induced apoptosis in Huvec and Jurkat cells and their antioxidative effects
… apoptosis of human umbilical vein endothelial cells (Huvec) and Jurkat cells in a concentration of 100 µM and 1mM respectively, when applied 2 hours prior to TNF-alpha exposition. To investigate molecular consequences on cellular signal transduction pathways, NF-kappaB reporter gene and DNA …
-
The role of membrane potential dynamics in cell behaviours : investigating the membrane potential dynamics in the Jurkat and HMEC-1 cell lines using the continuous wavelet transform
… the generation of action potentials in excitable cells, however, experimental evidence suggests that this membrane potential is also linked to various behaviours in all cells (Blackiston et al., 2009). These cell behaviours include cell proliferation, cell migration and even cell survival. The …
-
Signalling reactions of PI3Kgamma in T cells
… The involvement of PI3Ks in the activation of T cells is clearly demonstrated and so far mostly related to the PI3K class IA enzymes (alpha, beta and delta isoforms), but it was found that PI3Kgamma, the single member of class IB PI3K, is also involved in the regulation of activation-induced …
-
Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1
… induces apoptosis in a variety of tumor cells through activation of TRAIL-R1 (DR4) and TRAIL- R2 (DR5) with no effect on most normal cells. Also, Tumor cells that are resistant to TRAIL can be sensitized by combination treatment with chemotherapheutic agent or irradiation. But it has also …
-
Mutational Analysis of CD127 and Its Role in Immunological Diseases
… homeostasis and T-cell functions. In T-cells, IL-7 signals are transduced via IL-7's heterodimeric receptor composed of a common, γ chain (CD132) and an IL-7 specific, α chain (CD127). In light of the many roles that IL-7 plays in T-cell biology, it is no surprise that CD127 expression …
-
The role of the adapter protein TSAd in T cell activation
Introduction: T cells are a part of the adaptive immune system, which is designed to attack pathogens in the body. T cells recognize antigen presented by HLA molecules on the surface of antigen presenting cells (APC) through their T cell receptor. Conjugation formation between T cells and APC turns …
-
Nanosecond Pulsed Electric Field Induction of Programmed Cell Death Is Cell Type Dependent: An <i>in vitro</i> Study
… murine squamous cell carcinoma and human T-cell Jurkat clones that are wildtype, deficient in FADD (ΔFADD) and deficient in caspase-8 (ACas-8). The overall hypothesis is that nsPEFs eliminate cancer cells through activating caspase-dependent and caspase-independent programmed cell death pathways …
-
Reproducibility and reliability of chromatin immunoprecipitation in clinical research
… in a clinical population using white blood cells. It was established, for the first time, the normal variability of ChIP results in THP-1 cells, Jurkat cells, TF-1a, and human male circulating leukocytes over time for histone modifications H3k4me3 and H3k9Ac.</p>
-
Analysis of the aryl hydrocarbon receptor and a truncated form (AHR C[upper case symbol for greek Delta]Δ553) in cancer cells
… To test this hypothesis, we transfected HeLa cells with AhR CΔ553 fused to pEGFP or a vector under a tetracycline-inducible promoter. Stable cell lines expressing pEGFP-AhR CΔ553 have been generated and confirmed to have nuclear localization. We were also interested in confirming endogenous …
-
Development And Study Of Inhibitors Of Heat Shock Protein 70 Induction
Tumor and cancer cells that over express heat shock protein 70: HSP70) are found to be multidrug resistant and thermo tolerant, creating a hurdle to existing therapy. Although HSP70 is recognized as an increasingly important drug target, the protein structure of this highly conserved chaperone …
-
Phospholipase A2 Induced Monocyte Chemotaxis to Apoptotic Cells
… remodeling and maturation of hematopoietic cells. The clearance of apoptotic cells is essential to prevent autoimmune responses to sequestered antigens. This process is mediated by phagocytes of the monocyte lineage. Before phagocytosis can occur, macrophages must be recruited to the …
-
Studies of the Mechanisms of Reverse Signaling by CD40L
… protein expressed on activated CD4 T cells, traditionally known for its role in forward signaling by engaging CD40 on antigen- presenting cells to promote immune activation. While this pathway is well characterized, there is evidence that CD40L also mediates reverse signaling, acting …
-
NEW INSIGHTS INTO THE REGULATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION DURING APOPTOSIS
… receptor-mediated apoptosis. Distinct clones of Jurkat T-lymphocytes were used in which the mitochondria-mediated pathway had been inhibited at three different steps. The first aim investigated the molecular requirements necessary for Bak activation. Apaf-1-deficient cells and cells …
-
Design, Development, Characterization and Testing of CD22 Targeted Long Circulating Liposomal Drug Delivery Systems for B Cell Malignancies
<p>Hematological malignances of the B cells affect almost 130,000 people in the United States every year of which approximately 44,000 lose their lives. Therapies for B cell malignancies such as doxorubicin, have limitations due to dose related adverse effects such as neutropenia and …
-
Characterization and Development of Strategies for Altering Protein Expression in JSL1 Cells
… for regulating gene expression in eukaryotic cells. This process of differentially including or excluding variable exons provides a means for increasing proteome complexity. Alternative gene splicing occurs in a cell specific manner and may be influenced by changes in the extracellular …
-
Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… and maintenance of tissue homeostasis where cells induce their own cell death. Cells undergoing apoptosis exhibit a distinctive phenotype characterized by maintenance of membrane integrity, cell shrinkage, phosphatidylserine (PS) externalization at the plasma membrane, caspase protease …
-
Promoter Polymorphisms of Cysteinyl Leukotriene Receptor 1 (CysLTR1) Influence the Effect of Leukotriene Modifier on Transcriptional Regulation of the CysLTR1 Gene Induced by IL-4
… using lung carcinoma cell line, A549 cells, by real time PCR and flowcytometry. Effect of MK-571 on the transcriptional induction of CysLTR1 by IL-4 was examined by luciferase reporter assay. Results: In the screening of 5？ upstream region of CysLTR1 gene in 43 normal healthy controls, …
-
Protein sorting to the apical membrane of epithelial cells
… proteins to the apical membrane of polarised cells. Based on this, the aim of this work was to contribute to the understanding of apical sorting in epithelial cells. The study of how lipid rafts are structured has been hampered by the scarcity of techniques for their purification. Rafts are …
-
Enzymatic cleavage of chemokines CCL2 and CXCL10 : Implications for multiple sclerosis pathogenesis.
… contribute to the recruitment of immune cells into the CNS, and are expressed by astrocytes, the most abundant cell type in the CNS, and endothelial cells. CCL2 and CXCL10 are chemokines expressed in MS lesions, which are thought to be pivotal in chemoattraction of T cells and monocytes …
-
Expression of green fluorescent protein (GFP) in Leishmania spp. to identify survival strategies the parasite imposes on the human host
… as intracellular amastigotes within mammalian cells. Once inside host cells parasites they regulate and control immune responses from inside the host cells through the modulation of protein expression including cytokines. The changes involving Leishmania promastigote and host cells is well …
Page 1 of 2