Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"Jurkat"”.
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Understanding the biological barriers to Jurkat Transfection using non-viral peptides
… sulfate proteoglycans on the cell surface of Jurkat and primary T-cells weakens the cell entry of cationic nanoparticles. Besides, eliminated endosomal acidification in Primary T-cells limits the endosomal escape of transfection agents based on pH sensitiveness. <br/><br/>Next, RALA and …
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Regulation Mechanisms of TIM-3 expression in Jurkat T cells and HMC-1 cells
… TIM-3의 발현 조절 기작을 밝히기 위해 다양한 inhibitor들을 사용한 결과 Jurkat T 세포와 인간 CD4+ T 세포에서는 MEK 경로, HMC-1 세포에서는 MEK 경로와 SMAD 의존적 경로가 TIM-3 발현에 중요하게 작용함을 밝혔다. 또한 T 세포에서 TIM-3 발현 조절에 중요하게 작용하는 promoter 부분을 찾기 위해 다양한 길이의 TIM-3 promoter를 포함하는 luciferase reporter vector를 제작하여 luciferase 활성도를 측정하였다. 그 결과 TIM-3 …
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Le Rôle de la stéaroyl-CoA désaturase-1 dans le maintien de la prolifération des cellules T et de la lignée leucémique Jurkat
… la SCD-1 dans la lignée cellulaire leucémique Jurkat et dans les cellules T du sang périphérique. L'effet de l'inhibition de la SCD-1 sur les cellules T présente également un aperçu sur les répercussions possibles sur le système immunitaire. Nous avons développé un modèle de cellules …
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Nanosecond Pulsed Electric Field Induction of Programmed Cell Death Is Cell Type Dependent: An <i>in vitro</i> Study
… murine squamous cell carcinoma and human T-cell Jurkat clones that are wildtype, deficient in FADD (ΔFADD) and deficient in caspase-8 (ACas-8). The overall hypothesis is that nsPEFs eliminate cancer cells through activating caspase-dependent and caspase-independent programmed cell death pathways …
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The role of membrane potential dynamics in cell behaviours : investigating the membrane potential dynamics in the Jurkat and HMEC-1 cell lines using the continuous wavelet transform
… and oscillations in the membrane potential of Jurkat cells and HMEC-1 cells. The underlying nature of the membrane potential dynamics of Jurkat cells was investigated by perturbing the extracellular concentration of either K+ , Na+ or Cl- . The membrane potential dynamics of proliferating, …
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The role of the adapter protein TSAd in T cell activation
… and methods: Experiments were done using Jurkat TAg cells (T cell line) and Raji cells (B cell line). Transfection with electroporation was used to introduce different cDNA construct into the Jurkat cells. Conjugation of Jurkat cells and Raji cells was performed using SEE super antigen to …
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Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1
… of DR4-4 Fab was analyzed in various Jurkat mutant cell lines, such as FADD-deficient Jurkat cell lines, JNK-dominant negative (DN), c-JUN-DN, IkB-DN Jurkat cell lines. PI3K inhibitor WM was also used. To investigate the effect of calcium on DR4-4 Fab-induced cell death, Jurkat cells …
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Signalling reactions of PI3Kgamma in T cells
… the activation of TCR and IL-2 production in Jurkat cells, we used the PI3Kgamma specific inhibitor AS041164. In our experimental settings AS041164 did not exhibit any detectable cytotoxicity within the applied concentration range. Furthermore, this substance had no significant influence on …
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L' inhibition de SCD-1 chez la lignée leucémique Jurkat et les cellules T et caractérisation de l'expression des isoformes de la 5-Lipozygenase dans la polyarthrite rhumatoïde et les leucémies
… dans la lignée de cellules leucémiques Jurkat mais également sur les cellules T du sang périphérique afin d'avoir un aperçu des conséquences possibles sur le système immunitaire. Les effets de cette inhibition pharmacologique de SCD-1 ont été évalués dans la lignée leucémique …
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Studies on the Leukocyte-Associated Ig-Like Inhibitory Receptor-1 Signaling Pathway in T Lymphocytes
… LAIR-1 signaling, transformed human T cells (Jurkat) that overexpress LAIR-1 were treated with the constituent chain of collagen type II [α1(II)] to stimulate LAIR-1. LAIR-1 activation by α1(II) induced phosphorylation of both SHP-1 and SHP-2, as well as phosphorylation of LAIR-1. In addition, …
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Mutational Analysis of CD127 and Its Role in Immunological Diseases
… In developing my model, I decided to use Jurkat cells because they expressed high endogenous surface levels of CD132, low endogenous surface levels of CD127 and endogenous STAT5. Jurkat cells were transduced with lentiviruses that induced expression of either WT or one of the four mutant …
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Zinc Finger Protein 36L1 (ZFP36L1): Gene Expression, Regulation and Interactions with Immune Receptors in Human Tumour Cells.
… in several tumour cell lines such as THP1, Jurkat, B16, MCF7, and MC38. To study the function of ZFP36L1 a CRISPR-Cas9 system was used to generate ZFP36L1 knockout MC38 cell line. Our results show that ZFP36L1 limits production of TNF α and VEGF A. Our preliminary data also show …
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Development of screening assays to test novel integrin antagonists in allergic inflammation
… a primary antibody to integrin a4 expressed in Jurkat E6.1 cells. Levocabastine, but not chlorpheniramine, binds to a4b1 integrin and prevents eosinophil adhesion to VCAM-1, FN or human umbilical vein endothelial cells (HUVEC) cultured in vitro. Similarly, levocabastine affects …
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Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… at conditions that induce apoptosis in Jurkat and HL60 cells, (2) determine the response of HCT116p53+/+ cells to nsPEFs during DNA synthesis in respect to apoptosis and proliferation at conditions that induce apoptosis in Jurkat and HL60 cells; and (3) determine conditions of nsPEFs …
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La redistribution de l'acide arachidonique dans les glycérophospholipides des lymphocytes T humains
… de ces changements avec la lignée lymphocytaire Jurkat et avec les lymphocytes T quiescents qui ont cessé de proliférer. Le dernier objectif est de mesurer l'expression de certaines acyltransférases et phospholipases A2 chez les cellules T au repos et en prolifération."
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Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… (NFAT) transcriptional reporter assay in the Jurkat T-cell line, transient over-expression of CD37 was found to powerfully inhibit dectin-1 signalling following stimulation with its ligand, β-glucan. Over-expression of other tetraspanins also inhibited dectin-1 signalling, but did not globally …
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Charakterisierung der funktionalen Rolle von Cytohesin-1 in der LFA-1-vermittelten T-Zell-Adhäsion
… nicht mehr in der Lage war, die Adhäsion von Jurkat E6-Zellen an ICAM-1 stabil zu induzieren. Biochemische Interaktionsstudien konnten dabei zeigen, daß die Mutante weiterhin fähig war, die cytoplasmatische Domäne von CD18 zu binden. Diese und weitere Ergebnisse von Dr. W. Nagel, die einen …
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Studies of the Mechanisms of Reverse Signaling by CD40L
… using both primary human T cells and the Jurkat T lymphoblastoid cell line. Crosslinking CD40L with monoclonal antibody 5c8 induced upregulation of activation markers CD69 and CD25, ERK1/2 phosphorylation, and secretion of key cytokines including IFN-a, IL-2, and TNF-a in primary CD4 T …
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Regulation and function of hyaluronan binding by CD44 in the immune system
… and increased hyaluronan binding was observed in Jurkat T cells, which became more susceptible to activation-induced cell death when transfected with mutant CD44. The extent of cell death was dependent upon both the hyaluronan binding ability of CD44 and the size of hyaluronan itself, with high …
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