Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Jak1"”.
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Untersuchungen zur gp130/Jak1-Interaktion
… associated kinases of the Janus family (Jak1, Jak2 and Tyk2). Since Jak1 plays the most important role the interaction of gp130 with Jak1 was investigated by means of mutagenesis of both molecules. The minimal binding region of Jak1 to gp130 was restricted to the membrane proximal 68 …
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Charakterisierung der Interaktion von gp130 und Jak1
… dissertation is the interaction of gp130 and Jak1. Two aspects of this interaction were the main focus: Using FRAP (fluorescence recovery after photobleaching), it was demonstrated that there the kinase Jak1 has the same mobility as gp130 regardless of its kinase activity. This means that …
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The role of JAK1 and JAK3 in CD8<sup>+</sup> effector T cells
… was to explore the role of the tyrosine kinases JAK1 and JAK3 in cytokine signalling, focusing on interleukin-2 signalling in CD8<sup>+</sup> effector T lymphocytes. Initial experiments compared the effects of the pan JAK1/JAK3 inhibitor tofacitinib, the selective JAK1 inhibitor GSK186, and the …
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Il-6/Jak1 Drives Pd-L1 Phosphorylation and Glycosylation to Promote Cancer Immune Evasion
… interleukin (IL)-6-activated Janus kinase 1 (JAK1) phosphorylates programmed death-ligand 1 (PD-L1)-Tyr112, leading to the recruitment of endoplasmic reticulum (ER)-associated N-glycosyltransferase, STT3A, which catalyzes the glycosylation of PD-L1, contributing to its stability. A positive …
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Identification of the molecular pathways that mediate cytokine induced immunopathology of the gut epithelium
… screen identified the Janus kinases (JAKs) JAK1 and JAK2 as the major kinases required for IFN-γ + TNF-α dependent synergistic cell death. While TNF receptor-1 (TNFR1) was also required, there was no direct dependency of cell death on TNFR1-downstream canonical apoptotic and necroptotic …
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Die Regulation der Expression des humanen Oncostatin-M-Rezeptors durch Januskinasen
… region. In case of the OSMR, activation of Jak1, Jak2 and Tyk2 have been reported. Apart from their function in OSM signal transduction, a novel role of Jaks could be established in the present study: Upon heterologous expression of the OSMR in several cell lines, its surface expression was …
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Identification of the presence and activity of the JAK-STAT pathway in canine solid tumors
… was performed to evaluate protein levels of JAK1, phospho-JAK1, JAK2, phospho-JAK2, STAT3, and phospho-STAT3. Signalment, treatment information, and survival information was obtained from the medical record for each case. Results: Tumor samples were scored for percent positive neoplastic …
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Analyse der Wechselwirkungen von Proteinen des Interleukin-6-Signalwegs in einzelnen Zellen
… IL-6-type cytokines gp130 and the Janus kinase Jak1 was revealed by fluorescence recovery after photobleaching (FRAP) analysis. Signal transduction through cytokine receptors is mediated mainly by non-covalently associated Jak tyrosine kinases. By confocal microscopy, the cytokine receptor gp130 …
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Negative Regulation der Oncostatin M-vermittelten Signaltransduktion
… of SOCS3 depends on a direct interaction with JAK1. So far such a direct interaction has only been shown with overexpressed proteins and thought to be physiologically irrelevant due to the weak affinity of SOCS3 to the phosphorylated kinase activation loop. The second part of this study deals …
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Modulation der IL-6-abhängigen Signaltransduktion durch die Protein-Tyrosinphosphatase SHP2
… When human fibrosarcoma cell lines deficient in Jak1, Jak2 or tyrosine kinase 2 (Tyk2) were stimulated with IL-6/soluble IL-6R complexes it was found that only in Jak1-, but not in Jak2- or Tyk2-deficient cells, SHP2 activation was greatly impaired. It is concluded that Jak1 is required for the …
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Analysis of Human Interferon Signaling and its Regulation
… signaling cascade. The active Janus kinases JAK1 and tyrosine kinase 2 (TYK2) phosphorylate signal transducer and activator of transcription 1 (STAT1) and STAT2, which act as a transcription factor together with IFN-regulatory factor 9 (IRF9) to induce antiviral IFN-stimulated genes (ISGs). …
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Charakterisierung der Oncostatin-M-vermittelten STAT5-Aktivierung : Beitrag des Rezeptorkomplexes und der Januskinasen
… for the association with the janus kinases.- JAK1 and JAK2, which bind to the OSMR, are both involved in STAT5-phosphorylation: JAK1 is mandatory, whereas JAK2 further enhances STAT5 activation.- If JAK2 lacks its kinase activity or the OSMR its YLYLLP-motif, STAT5 activation is reduced to …
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Relevanz der Transmembran- und Intrazellulärregion von gp130, LIFR und OSMR für deren Liganden-induzierte Signaltransduktion
… region of the LIFR recruits the Janus kinases Jak1, Jak2 and Tyk2; the membrane distal tyrosine residues are sufficient for activation of the transcription factors STAT1 and STAT3. The OSMR in contrast recruits via its cytoplasmatic tyrosine residue 861 specifically the adapter protein Shc. …
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Expression und Aufreinigung von intrazellulären Anteilen des Interleukin-4-Rezeptors als GST-Fusionsproteine und Messung von Protein-Protein-Wechselwirkungen
… Für GST-E1 wurde eine Wechselwirkung mit Jak1 nachgewiesen, die dem bisherigen Kenntnisstand entspricht; für GST-ginP5D4 hingegen konnte eine Wechselwirkung mit Jak3 nicht gezeigt werden. Beide Proteine sind in der Lage, STAT5 zu präzipitieren; diese Bindung erscheint unabhängig von der …
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Cochlear Sensitization to Aminoglycosides: Defining the Role of JAK-STAT Signaling
… alone. In adult mouse models, systemic dual JAK1/2 inhibition with momelotinib worsened hearing loss and cochlear damage in an inflammatory kanamycin paradigm. Subsequent kinase specific pharmacologic studies refined this finding: systemic JAK1-biased inhibition with upadacitinib or …
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Systematic Enhancement of Human Herpesvirus 6A Reconstitution: Bridging Technical Refinement and Host Determinants of Virus Replication
… reconstitution, highlighting the potential of JAK1/JAK2 inhibition via ruxolitinib (RUX) to improve viral yields. By systematically optimizing nucleofection and stimulation conditions, we developed a robust and reproducible methodology that significantly enhances virus reconstitution. Our …
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Role of JAK3 in Myogenic Differentiation
… (MRFs) family and many signaling pathways. The JAK1 and JAK2 pathways are known to each have different effects on myoblast proliferation and differentiation; however, the role of JAK3 in myoblast differentiation remains unclear. In this study, we investigated the effect of JAK3 inhibition on …
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The Regulation of Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase 2 (SHP-2) in Brain Microglia
… the phosphorylation of STAT1/3 as well as JAK1/2 in microglia activated with gangliosides, LPS, or IFN-γ. Curcumin inhibits the expression of inflammation-associated genes, including ICAM-1 and MCP-1, whose promoters contain STAT-binding elements. To test the inhibitory mechanism of …
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Determinants of systemic treatment response in atopic dermatitis
… and translational study examined selective JAK1 inhibition with abrocitinib. Rapid clinical improvement was accompanied by restoration of barrier integrity, downregulation of inflammatory proteins, and normalization of skin physiology and microbial diversity.
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