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Showing 1 to 7 of 7 for “"JEG-3 cells"”.
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Human Gonadotropin-Releasing Hormone (hGnRH) Gene Expression and Hormone Regulation in Human Placental JEG-3 Cells
<p>Using the human placental choriocarcinoma JEG-3 cell line as an <em>in vitro</em> human placental model, I studied the mechanisms of the tissue-specific expression and steroid hormone regulation of the hGnRH gene in the human placenta. The results showed that all of the previously identified …
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Tissue-Specific Expression and Steroid Hormone Regulation of Human Gonadotropin -Releasing Hormone (hGnRH) Gene in Placental Cells (JEG -3 cells)
<p>Using JEG-3 cells as an <em>in vitro</em> model, I investigated the mechanisms behind the tissue-specific expression and steroid hormone regulation of the hGnRH gene in the human placenta. The hGnRH upstream promoter was found to be functionally active in JEG-3 cells. The DNA sequence …
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Ryanodine receptor expression in trophoblasts
Trophoblasts of the placenta are the frontline cells involved in communication and exchange of materials between the mother and fetus. Within trophoblasts, calcium signalling proteins are richly expressed. Intracellular free calcium ions are a key second messenger, regulating various cellular …
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Mapping the immunological receptors CD14 and macrophage receptor with collagenous structure (MARCO) and their innate recognition potential on trophoblast cells: relevance for human pregnancy
… is that MARCO might be expressed on trophoblast cells and plays a valuable role in association with CD14. The interaction of CD14 and MARCO was explored with the use of confocal microscopy which showed physical associations, most likely contributing to their function at the feto-maternal …
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The role of soluble factors affecting the major histocompatibility complex class I molecules In an IN VITRO model of the fetomaternal interface
… on the cell surface of almost all somatic cells and is related to the presentation of self-antigens. Class I can be sub-divided into two groups known as classical class I (Ia) and non-classical class I (Ib), and expressed on the cell surface as human leukocyte antigen (HLA). HLA-G is one of …
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Prenatal stress, the placenta and maternal microbial transmission; implications for health and disease
… We first utilized an in vitro placenta model, JEG-3 cells, to examine the effects of stress on the placental expression of HSD11B2. JEG-3 cells were cultured with exogenous cortisol and interleukin-1 beta (IL-1β), two potential biological mediators of prenatal stress. This study showed both …
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Estrogen Regulation of the Pregnant Baboon Placental 11β-Hydroxysteroid Dehydrogenase (11β-HSD)-Catalyzed Metabolism of Cortisol and Cortisone and Its Effect on the Development of the Fetal Hypothalamic-Pituitary-Adrenal Axis
… and transiently-transfected into human placental JEG-3 cells. In the presence of 17β-estradiol and estrogen receptor α, basal promoter activities of both 11β-HSD-1 and -2 increased 8-fold. Finally, RT-PCR analysis demonstrated a significant reduction of both 11β-HSD-1 and -2 mRNAs in baboon …