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Showing 1 to 16 of 16 for “"J774A.1"”.
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The Identification of the MCHR-1 in J774A.1 Macrophages and The Effect of Melanin-concentrating Hormone on a 3T3-L1 Adipocyte-J774A.1 Macrophage Co-Culture
… and acrophages in our laboratory. First, the J774A.1 macrophage line was acquired. Eventually, successful macrophage culturing technique was learned. Then, I asked whether theses J774A.1 macrophage expressed the Mchr1 gene and MCH receptor protein as well as whether this cell line responded at …
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Modular Nanoparticles for Selective Cell Targeting
… DS coated NPs could be readily internalized by J774A.1 monocytes/macrophages, whereas PEGylated NPs effectively reduced cellular uptake/recognition. The versatility of this NP system was further demonstrated by laying DS on the Hep/PLL NP surface. DS-coated NPs were recognized by J774A.1 cells …
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Developing an in vitro model to study trained immunity
… in vitro cell model to study trained immunity. J774A.1 macrophages and THP-1 monocytes were evaluated as murine and human models, respectively, to study trained immunity in vitro. THP-1 monocytes were differentiated from monocytes to macrophages with 72-hour phorbol 12-myristate-13-acetate (PMA) …
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Establishment of a Long Term Cell Culture Model for Testing Anti-Infectives against Mycobacterium avium subsp. paratuberculosis
… and do not mimic disease observed in ruminants. J774A.1 murine macrophages typically have a very short lifespan of about 4-6 days, however MAP infected cell cultures can survive up to about 10 days. Using a modified protocol of Estrella et al. (2011), we have been able to establish a 45-60 day …
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Evaluating Immunotoxicity of Quaternary Ammonium Compounds
… We assessed immunotoxicity in-vitro with J774A.1 murine macrophage cell line by analyzing cytokine production and phagocytosis; and evaluated developmental immunotoxicity in CD-1 mice by analyzing antibody production. Additionally, because of the associations between gut microbiome …
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Study of the antioxidant, anti-inflammatory and anti-diabetic effects of extracts from Cotinus coggygria in models with experimental animals and cell cultures // Проучване на антиоксидантното,противовъзпалително и противодиабетно действие на екстракти от Cotinus coggygria в експериментални модели с опитни животни и клетъчни култури
… cell cultures: 3T3-L1 (murine preadipocytes) and J774A.1 (murine macrophages);5)treatment of male Wistar rats with three aqueous extract from the leaves and aqueous-ethanolic extract of wood in a model of subchronic toxicity (30 days); (6) Study of in vivo antioxidant properties of the extracts …
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Study of the Metabolic Effects of Water-alcoholic Extracts of Heartwoods with Traditional Use in Bulgaria // Проучване метаболитните ефекти на водно-алкохолни екстракти от дървесини с традиционно приложение в България
… inflammation in murine 3T3-L1 preadipocytes and J774A.1 macrophages. Based on the results obtained it could be suggested that mulberry heartwood ethanol infusion possesses antioxidant activity by inhibiting transcription levels of enzymes related to the endogenous antioxidant defense (GCL and …
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Role of entF Gene in Iron Acquisition by Brucella abortus 2308
… inhibition of BAN1 mutant was found in murine J774A.1 macrophages, which suggests that Brucella could acquire iron inside mammalian cells. The entF gene complemented mutant strains of BAN1 (BAN2A and BAN2B) were found to be intermediate in their ability to grow in iron minimal media …
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Development of novel strategies to combat multidrug resistance mediated by efflux transporters and intracellular bacteria
… and Brucella abortus infection within J774A.1 macrophage cells by 62% and 90% respectively, with no concomitant toxicity towards the host cells.</p>
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Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol
… using in vitro murine macrophage cell line j774a.1 and polymorphonuclear cells (pmnc's) from five crohn's disease patients. Pmnc's from three healthy individuals and two ulcerative colitis patients, as well as escherichia coli (atcc 11303) and mycobacterium tuberculosis strain h37ra (atcc …
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Development of Drug Loaded Nanoparticles for Treatment of Mycobacterium avium Infection
… of drug-laden nanoparticles were non-toxic to J774A.1 mouse macrophage cells at therapeutic doses. In vivo efficacy studies showed that only amikacin-loaded polymeric nanoparticles improved clearance compared to free amikacin in M. avium infected BALB/c mice. In general, none of the …
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Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… cells, bone marrow-derived macrophages, and J774A.1 cells (herein referred to as J774 cells) (commonly used macrophage-like cell line) via morphology, flow cytometry, mass spectrometry-based proteomics analysis, and comparison of key phagosomal parameters (phagocytic index, phagosomal …
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Augmenting Anti-Tumor Immunity by Targeting Macrophage Cox-2 in Breast Cancer
… evident in lipopolysaccharide (LPS)-stimulated J774A.1 cells. Importantly, urinary prostaglandin levels were not altered in mice treated with rHDL-celecoxib, suggesting no systemic inhibition of COX-2 with this targeted approach. These studies provide rationale for targeting macrophage COX-2 in …
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MicroRNA-mediated Attenuation of Inflammation in NZB/W Lupus Mice
… proliferation and NFκB activation in J774A.1 macrophages and MES 13 mesangial cells in vitro. Cell proliferation, retinoblastoma protein (Rb) phosphorylation, and NFκB activation were increased in cells transfected with miR-let-7a and stimulated with LPS/IFN-γ. Expression and …
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Characterization, toxicity, and biological activities of organometallic compounds and peptide nucleic acids for potential use as antimicrobials
… of novel OMCs by evaluating their effects on J774A.1 murine macrophage-like cells that were either uninfected or were infected with Mycobacterium bovis BCG. Results indicate that OMCs with an iridium (Ir) metal center and an amino acid ligand show minimal cytotoxicity against eukaryotic cells …