Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"J774"”.
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The Interactions of Clostridium Perfringens With Phagocytic Cells
… We found C. perfringens was not killed by J774-33 cells or mouse peritoneal macrophages under aerobic or anaerobic conditions. Using electron microscopy, we showed that C. perfringens could escape the phagosome of J774-33 and mouse peritoneal macrophages. We believe the ability of C. …
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Inhibition of Heat Shock Protein 90 Reduces Inflammatory Signal Transduction in Murine J774 Macrophage Cells and Lessens Disease in Autoimmune MRL/lpr Mice: What in vitro, in vivo, and in silico Models Reveal
… signal cascade levels. To test this, J774 mouse macrophage cells were treated with 17-DMAG and immune-stimulated with lipopolysaccharide (LPS). 17-DMAG treatment reduced nitric oxide (NO) production and the expression of pro-inflammatory cytokines interleukin (IL)-6, IL-12, and TNF-α. …
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Captura del óxido nítrico por ditiocarbamatos de cobre (II). Regulación de su contenido en los sistemas vivos
… producido in vivo por células macrófagos murinos J774 estimulados por LPS e IFNγ.
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Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… cells, bone marrow-derived macrophages, and J774A.1 cells (herein referred to as J774 cells) (commonly used macrophage-like cell line) via morphology, flow cytometry, mass spectrometry-based proteomics analysis, and comparison of key phagosomal parameters (phagocytic index, phagosomal …
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Untersuchungen zur Aufklärung der In-vivo-Funktion des Zytolysins ClyA von Escherichia coli
… entsprechenden clyA-Mutante unter Verwendung von J774-Makrophagen durchgeführt wurden, ergaben außerdem, daß ClyA wesentlich zur Zytotoxizität des wildtypischen EIEC-Stamms beiträgt. Eine spezielle Bedeutung von ClyA für die fakultativ intrazelluläre Lebensweise von EIECs konnte mit diesen …
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Dietary fatty acids modulate inflammatory cytokine production through activation of MAP kinases
… of innate immune responses. Ths study used the J774 munne macrophage cell line as a model. The results demonstrate that PUFA exert distinct effects on both pro-inflammatory and anti-inflammatory cytokine production depending on whether the cell is achvated via TLR2 or TLR4 Thus, providing …
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Delivery and stability of antisense oligonucleotide conjugates in vitro
… of this conjugate was enhanced to RAW264.7 and J774 macrophage cell lines via a mechanism of receptor mediated endocytosis. The delivery of three lipophilic ODN conjugates, cholesterol (cholhex), 16-carbon alkyl chain (C16) and hexa-ethylene glycol (HEG) moieties and an unconjugated ODN were …
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HDAC6 Deletion Decreases Pristane-Induced Inflammation and Lupus
… compared to the WT mice. In vitro studies in J774 cells revealed that the selective HDAC6 inhibitor (ACY-738) increased acetylation of NF-κB while increasing STAT1-phosphorylation which caused the synthesis of inducible nitric oxide synthase (iNOS) in cells activated by LPS and IFN-γ. …
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Óleo fixo do Syagrus coronata : segurança do uso, reparo tecidual em ferida bucal e atividade antimutagênica
… três linhagens de células analisadas (HEK-293, J774.A1 e Sarcoma-180). No ensaio de toxicidade oral, foi identificado através da análise bioquímica redução da glicose, ureia e proteínas totais nos grupos tratados com óleo. Na histomorfometria, células endoteliais do fígado apresentaram …
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Antibody Dependent Enhancement of Visceral Leishmaniasis
… uptake by macrophages <em>in vitro</em>. J774 macrophages were combined with either <em>L. major</em> or <em>L. infantum</em> in the presence of <em>L. major</em> antiserum, naïve serum, or no serum. Parasite uptake was measured at several time points using flow cytometry. In the presence …
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From yeast to animals: A small molecule restores iron mobilization, absorption and hemoglobinization
… mediated iron transport by first loading J774 Mouse Macrophages with iron, adding hinokitiol and watching release of iron, followed by addition of a large excess of iron to the extracellular media. Upon addition of the extracellular iron, the direction reversed from exporting iron out of …
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Ochrobactrum anthropi: a soil bacterium for the study of Brucella virulence
… and survived longer in the macrophage J774 A.1 cell line. The control O. anthropi was cleared from BALB/c mice in five days while the recombinants were recovered from spleens, livers and lungs of infected mice up to eight days post-infection. We tested the contribution of B. suis cyclic …
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Restored iron transport by a small molecule promotes absorption and hemoglobinization: discovery, development, and mechanistic studies
… product also enabled the movement of iron out of J774 macrophages deficient in ferroportin. The same compound promotes gut iron absorption in DMT1-deficient rats and ferroportin-deficient mice, as well as hemoglobinization in DMT1- and mitoferrin-deficient zebrafish. These findings illuminate a …
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Characterization of Phagosome-Lysosome Fusion in Macrophages
… PMA was also observed by a macrophage cell line, J774, and to a lesser extent by primary rat embryo fibroblasts. P-L fusion was markedly inhibited by several days of culture in various polyanionic compounds such as dextran sulfate (DS), which were endocytosed and concentrated in secondary …
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Intracellular growth of Brucella abortus and B. melitensis in murine macrophage-like cell lines and partial characterization of a biologically active extract from B. abortus strain RB51
… growth in one murine macrophage-like cell line (J774.A1) clearance pattern reflected the in vivo growth kinetics of the various Brucella tested. All strains tested in the macrophage model had a significant 1-4 log decrease in intracellular bacteria at 24 hours post infection. The decrease in …
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Pharmacokinetic and Pharmacodynamic Studies of a Novel Spectinamide Series of Antituberculosis Agents
… into <em>Mycobacterium bovis</em> BCG and into J774 murine macrophages were performed to understand reasons for improved activity of spectinamides and to evaluate their potential to target primary TB infection that resides in macrophages. <em>In vivo </em>pharmacokinetic studies were carried out …
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Analyse der Reifung von Afipien- und Rhodokokken-enthaltenden Phagosomen in Makrophagen
… der Afipia felis-enthaltenden Phagosomen in den J774E-Makrophagen. Somit konnte nachgewiesen werden, dass die Mehrzahl der Phagosomen (70 Prozent), die Afipia felis enthalten, nicht zum endozytischen System gehören. Diese ungewöhnliche Kompartimentierung besteht bereits bei der Aufnahme und kann …