Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Iswi"”.
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The role of the chromatin remodeller ISWI in the cellular response to hypoxia
… enzymes. This thesis describes the role of ISWI (Imitation Switch) in the cellular response to hypoxia. It was found that unlike SWI/SNF, ISWI depletion enhances HIF activity without altering its levels. Further analysis revealed that ISWI acts in a specific manner on certain HIF target …
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MECHANISMS OF CHROMATIN REMODELING BY ISWI FAMILY OF REMODELERS: A FUNCTIONAL AND STRUCTURAL INSIGHT INTO THE ROLE OF THE Itc1 SUBUNIT OF ISW2 REMODELING COMPLEX
ISWI type remodelers mobilize and space nucleosomes. These ATP-dependent remodeling complexes have a relatively small number of subunits (2-4) as compared to other classes of remodelers such as SWI/SNF, RSC and INO80/SWR-C. The accessory subunits of some of the ISWI remodelers from yeast have been …
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DISTINCT GENOME WIDE FUNCTIONS OF CHROMATIN REMODELERS IN NUCLEOSOME ORGANIZATION AND TRANSCRIPTION REGULATION
… In this study, we investigated the role yeast ISWI and SWI/SNF family of chromatin remodelers play on nucleosome rearrangement and transcription regulation by targeted mutagenesis of domains in accessory subunits and at the C-terminus of the catalytic subunit. All members of the ISWI family …
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The role of aesthetics in energy-retrofit strategies: the case of solid wall houses in the UK
… the uptake of Internal Solid Wall Insulation (ISWI)demand in the energy technology industry.Two interconnected gaps in the literature were identified. One gap is the lackof clear information on the performance benefits of SWI as a single retrofitmeasure in solid wall homes, which is a cause of …
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THE MECHANISMS OF ISW1A, ISW1B AND ISW2 CHROMATIN REMODELERS
… the nucleosome remodeling mechanism of three ISWI complexes in yeast using various biochemical approaches. The aspects investigated were, identification of the suitable substrate for ISW1a, role of accessory subunits and various domains/motifs of ISWI complexes in nucleosome remodeling, and …
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ROLES OF ACCESSORY DOMAINS IN THE CATALYTIC SUBUNIT OF ISW2
… remodeler complexes. ISW2 belongs to the ISWI family of chromatin remodelers in Saccharomyces cerevisiae and has been shown to be involved in transcription repression and DNA replication. The catalytic subunit of ISW2 consists of the ATPase domain and three domains at the C-terminus called …
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STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE ISW2 CHROMATIN REMODELING COMPLEX
… from ATP hydrolysis to mobilize nucleosomes. ISWI remodelers mobilize and evenly space nucleosomes to regulate gene expression. ISW2, an ISWI remodeler in yeast, has been shown to reposition nucleosome near promoter regions and represses both mRNA and antisense non coding RNA transcription. …
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MODES OF NUCLEOSOME INTERACTION AND MECHANISMS OF THE SACCHAROMYCES CEREVISIAE CHROMATIN REMODELERS INO80 AND ISW1A
… (ATPase) domains of remodelers belonging to the ISWI and SWI/SNF families translocate along DNA close to the center of nucleosomes in order to mobilize, space or disassemble nucleosomes. In contrast, we observed that INO80 has a strikingly distinct mechanism, which is different even from its …
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A functional role of chromatin remodeling factor, Rsf-1 in the DNA damage signaling pathway
As a member of imitation switch (ISWI) family in ATP-dependent chromatin remodeling factors, RSF complex consists of SNF2h ATPase and Rsf-1. Although it has been reported that SNF2h ATPase is recruited to DNA damage sites (DSBs) in poly(ADP-ribosyl) polymerase 1 (PARP1)-dependent manner in DNA …
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Colocalization between Akirin, the NuRD Complex, and Histone Modifications
… chromatin remodeling complexes, SWI/SNF, INO80, ISWI, and CHD, we know Akirin interacts with subunits of the SWI/SNF family Brahma (BRM) chromatin remodeling complex during myogenesis and cardiogenesis. Interaction between Akirin and CHD family chromatin remodeling complexes has not been …
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AN INVESTIGATION OF THE MECHANISMS OF GROUCHO MEDIATED TRANSCRIPTIONAL REPRESSION
… Psq total (antibody against all Psq isoforms), ISWI, and CBP. A weaker signal was observed for BTB-containing Psq, and Pc, a very weak signal for the architectural proteins Su(Hw), CP190 and Mod(mdg4). Thus, these results suggest a preferent co-localization of Gro with non-BTB Psq and their …
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LINKING CHROMATIN REMODELING TO MITOCHONDRIAL DYSFUNCTION: A ROLE FOR THE SMARCA5-MYC-TFAM AXIS IN HEPATOCELLULAR CARCINOMA
… 5), an ATP-dependent chromatin remodeler of the ISWI family, as essential gene for maintaining mitochondrial function, proliferation, and viability in HCC cells. Depletion of SMARCA5 led to severe impairments in mitochondrial respiration, reduced ATP production, and widespread disruption of …
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Mechanism of Human Papillomavirus E6-Mediated Repression of P53-Target Gene *Transcription
… purified chromatin assembly factors (Acf1 and ISWI), core histones (H2A, H2B, H3, and H4), and a histone chaperone (NAP-1) in order to define the mechanism used by high-risk and low-risk HPV E6 to inhibit p53-dependent transcription. We demonstrate that E6 does not prevent p53 or p300 …
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THE UNIQUE STRUCTURE AND MECHANISM OF INO80 - AN ATP DEPENDENT REMODELER OF THE HISTONE EXCHANGER FAMILY
… or how it differs in comparison to SWI/SNF or ISWI. Site-directed mapping of histone-DNA contacts showed that INO80 generally remodels mononucleosomes by moving them to the center of DNA. The length of extranucleosomal DNA was found to play an important role in nucleosome binding as well as …
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Myxoid Liposarcoma: Models and Mechanisms of Sarcomagenesis and Response to Radiation Therapy
… the ATPase subunit of the imitation switch (ISWI) complex. Using knockdown systems, we demonstrated that these interacting chromatin remodelers are functionally important for proliferation specifically in FUS-CHOP-driven, but not Kras-driven murine sarcoma cells. ChIP-seq and CUT&RUN …
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The roles of DPPA2, DPPA4 and SMARCA5 in mouse zygotic genome activation, epigenetic reprogramming and development
… Chapter 5, I assess the role that the maternal ISWI ATPase SMARCA5 plays in embryonic development, ZGA and chromatin remodeling in early embryos. For this I use both a conditional knock-out mouse model lacking SMARCA5 in oocytes, but also a targeted protein depletion system in early wild-type …
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DYNAMICS OF NUCLEOSOME REMODELING BY ATP-DEPENDENT CHROMATIN REMODELERS
… are ATP-dependent chromatin remodelers- ISWI, SWI/SNF, INO80, and CHD/Mi2. All chromatin remodelers transduce the energy from ATP hydrolysis to translocate on DNA, break histone-DNA contacts, and mobilize nucleosomes. However, the final outcomes of nucleosome remodeling are diverse - …
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Roles of origin recognition complex subunit 6 (Orc6) in DNA replication and damage response
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-08-01
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SWI/SNF COMPLEXES COORDINATE WITH HISTONE MODIFICATIONS TO REGULATE CHROMATIN REMODELING
… data suggest a similarity between SWI/SNF and ISWI class of chromatin remodelers based on their dependence on the H4 tail. Owing to the presence of acetyl-lysine binding bromodomains in these complexes and to a greater extent in RSC the differences in their remodeling activities, if any, were …