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Showing 1 to 20 of 34 for “"Isotopic labeling"”.
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Osmium alkane complexes: Synthesis, characterization, and isotopic labeling studies
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01
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Examining Molecular Interactions of Proteins by Isotopic Labeling, Sample Formulation and Solid-State NMR Spectroscopy
… procedures by using a pair-wise amino acid labeling technique. Using auxotroph E. coli strains, unambiguous amino acid pair assignments in the 144 kDa cytochrome bo3 oxidase was possible. This strategy will enable identification of key residues in the active site of this large enzyme, and …
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Isotopic labeling of isoflavones from plant cell and organ culture for metabolic tracking in animal models
Kudzu and red clover isoflavones are phytoestrogens that have been investigated as alternatives to estrogen replacement for treatment of osteoporosis, cardiovascular disease, and neurological disorders. However, the pharmacokinetics of these compounds are not clearly understood. In the present …
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Hydrogenase Models and Studies Based on Iron Carbonyl Chelate Complexes
… or comproportionation. The routes readily allow isotopic-labeling of model complexes with 57Fe for study by specialized techniques, e.g. Mossbauer or NRVS, generating new insights into [FeFe]- and Hmd-H 2ases. A comproportionation route permits selective labeling of one iron center of the diiron …
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SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… resistance. In addition, using a chemoenzymatic isotopic labeling strategy, we observed by NMR a slight modification of the motions within the bacterial rRNA in complex with one of the compounds. We further made use of various isotope labels for the partial resonance assignment of the human …
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In pursuit of a chemical and phenomenological understanding of long-living atmospheric pressure water-based ball plasmoids
… H2O, NH3, and NO3 bound to metal ions. Using isotopic labeling, these ions provided quantitative evidence showing the plasmoid’s unique ability to protect its contents from mixing with the ambient air.
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Structure determination of proteins and protein aggregates by magic-angle spinning solid-state NMR
… combining new pulse sequences with advanced isotopic labeling schemes, and novel structure calculation methods. The techniques developed in this study will be useful in the study of other protein aggregates, as well as membrane proteins and complexes, for which SSNMR is the structure …
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Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
… of MFA, as carried out via stable isotope labeling and GC/MS measurements, is the large number of isotopomer equations that need to be solved. This restriction reduces the ability of MFA to fully utilize the power of multiple isotopic tracers in elucidating the physiology of complex …
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The conformational landscape of RNA translational regulators and their potential as drug discovery targets
… structure determination by NMR: the high cost of isotopic labeling, tedious and ambiguous resonance assignment methods, and a limited database of RNA optimized pulse programs. We have addressed some of these challenges to NMR characterization of RNA structure with applications to various RNA-drug …
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6- and 5-Halodecaboranes: Selective Syntheses From ClOSO-B10H10(2-) and Use as Polyborane Building Blocks
… explained computationally and confirmed through isotopic-labeling studies. The regeneration of the polymeric products of ammonia-borane dehydrogenation was carried out through a process that included digestion of the polymer, complexation of the digestate with a base, reduction of B-X bonds to …
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Characterization of Cyclopropyl Synthases Involved in the Maturation of Ribosomally Synthesized and Posttranslationally Modified Peptides
… a variety of chromatography, mass spectrometry, isotopic labeling, and NMR spectroscopy techniques, we show that TigE catalyzes the formation of C-C bond between the γ-carbons on TigB isoleucine residues (Ile), forming a methyl-cyclopropyl glycine (mCPG). Using crystallography and site-directed …
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Biochemical Characterization of the DUF3328 Protein in the Biosynthesis of Cyclic Peptide Cyclochlorotine
… for the activity. Mechanistic studies using isotopic labeling confirmed dioxygen as the oxygen source. Copper redox cycling was found to be essential, with Cu(I) required for the catalysis. This work establishes DUF3328 proteins as a new class of copper-dependent enzymes involved in fungal …
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High oxidation state molybdenum and tungsten imido alkylidene and metallacycle chemistry : catalytic asymmetric olefin metathesis and mechanistic investigation
… complex, was structurally characterized. Isotopic labeling experiments with ¹³C₂H₄ allowed the observation of
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Boundary Layer Dynamics in Mixed Hydrocarbon Thin Films on Metal Surfaces
… features are discussed in detail. Using isotopic labeling of one component in the bilayer allows the dynamics of each component to be differentiated, demonstrating the mobility of the molecules as a function of temperature. Temperature programmed desorption spectroscopy provides …
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The selective oligomerization of ethylene with metal-organic frameworks
… 3 details a combination of studies utilizing isotopic labeling and mechanistic probes to demonstrate that Ni-MFU-4/ dimerizes ethylene via the Cossee-Arlman mechanism. Chapter 4 reports the preparation of Ni-CFA-1, a related heterogeneous ethylene dimerization catalyst that is far more …
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Synthesis and small molecule chemistry of the niobaziridine-hydride functional group
… activation processes has been obtained through isotopic labeling (H/D) studies. To further ascertain the propensity of la- H to serve as a masked form of 2a, its reactivity with small-molecule substrates was surveyed. Treatment of la-H with nitrous oxide (N₂0) or triphenylphosphine oxide …
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Magic angle spinning NMR applied to membrane protein 2D crystals : the structure and function of VDAC
… (2D) crystals. A strategy combining specific isotopic labeling with advanced 3D heteronuclear experiments enabled assignment of a large extent of the primary sequence. Extensive assignments of VDAC in lipid bilayers permitted comparison of VDAC's secondary structure between lipid bilayers and …
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Mass Spectrometry for Determination of Conformation and Dynamics of Proteins and Structure and Biosynthesis of Bacterial Peptidoglycan
… Furthermore, we developed a time-dependent isotopic labeling strategy and applied it to <italic>E. faecium</italic> during the cell wall growth to determine quantitatively the percentage of heavy isotope incorporation into different muropeptides through peptidoglycan growth cycles, discussed …
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Development of biophysical and Mass Spectrometry Assays for APOBEC3 cytosine deaminases: discovery and validation of cnvalent and non-covalent ligands
… non-specific protein thiol reactivity through isotopic labeling. Appendix A describes a previously undescribed phenomenon where the A3A inactivating mutation E72Q destabilizes protein tertiary structure, where the E72A inactivating mutation does not. Appendix B discusses efforts at …
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Exploring the Biophysical Properties of Domains Within Multi-Domain Proteins
… have also utilized this technique and segmental isotopic labeling to study the Cterminal SH3 (cSH3) domain of the signaling adaptor protein, Crk-II. Several studies suggest that the cSH3 domain plays an important regulatory role in the protein. However, no structural information is available on …
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