Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Isotachophoresis"”.
-
Transverse Isotachophoresis Using Polyacrylamide Gel Electrodes
… the isolation of nucleic acids using transverse isotachophoresis (ITP). A boro-silicate glass chip is used with Poly(Acrylamide) gel electrodes to establish an electric field perpendicular to the direction of sample flow, causing a controlled migration of charged particles. The design and …
-
CE-LIF ANALYSIS OF INTACT MARINE MICROBES ALONG WITH THEIR CONSTITUENT PHYCOBILIPROTEINS AND PHYCOBILIN PIGMENTS
… known as polymer enhanced capillary transient isotachophoresis (PectI). Four phycobiliproteins (R-PE, B-PE, C-PC, and APC) were determined by CE and PectI, with detection limits below 10 ppb achieved for each protein. Additionally, the phycobilin pigments giving rise to the native fluorescence …
-
Enhanced NMR Detection of Mass -Limited Samples With Microcoil Probes: Increasing Sample Throughput and Improved on-Line Coupling to Microscale Separations
… microcoil NMR has been coupled to capillary isotachophoresis (cITP), a form of capillary electrophoresis that can concentrate charged analytes over 100-fold. Consequently, for dilute charged analytes buried in complex mixtures, cITP can isolate and concentrate the charged analytes before …
-
High fidelity simulations of electrokinetic phenomena in microfluidic devices
… on simulations of two electrokinetic phenomena, isotachophoresis (ITP) and electrokinetic instability (EKI). A set of coupled governing equations including the incompressible Naiver-Stokes equations, Nernst-Planck transport equations and a charge conservation equation are solved in the …
-
Direct Quantitative Analysis of Multiple Micrornas
… the limit of detection I combined DQAMmiR with isotachophoresis, an in-capillary pre-concentration technique. The limit of detection improved by two orders of magnitude (from 100 pM down to 1 pM), allowing the detection of low abundance miRNAs. To improve the specificity of DQAMmiR I …
-
Analysis of Clinically Important Compounds Using Electrophoretic Separation Techniques Coupled to Time-of-Flight Mass Spectrometry
… methods - field amplified injection (FAI) and isotachophoresis (ITP) - to capillary zone electrophoresis with both UV and MS detection was evaluated. Using FAI-CE-UV, angiotensins were detected at ~1 nM concentrations. Using similar conditions but TOFMS detection, the detection limits were …
-
Analysis of plant analytes using capillary electrophoresis and high performance liquid chromatography
… and pre-concentration of amino acids using isotachophoresis (leading electrolyte = HCl, terminating electrolyte = hydroxyproline) was performed. The separation of amino acids was carried using acetic acid. For minimising uncontrolled hydrodynamic injection poly(ethylene oxide) was used. …
-
Determination of nicotine and its metabolites by capillary electrophoresis and mass spectrometry.
… metabolites in urine. A counterflow transient isotachophoresis (tITP) method was developed, with MS detection, for the analysis of even lower analyte concentrations. Limits of detection for both nicotine (0.03 mug/ mL) and cotinine (0.34 mug/ mL), via HE-tlTP/MS, were considerably lower than …
-
Using Capillary Electrophoresis for High-Throughput Molecular Screening
Advances in the development of DNA-encoded chemical libraries have necessitated rapid selection strategies to aid in molecular discovery. Encoded chemical libraries are composed of thousands of unique (potential) drug molecules, each encoded with a corresponding unique DNA sequence, allowing for …