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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 128 for “"Isomerase"”.
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Crystallography and mutogenesis triosephosphate isomerase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1990.
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Prolyl Isomerase Pin1 Is a Conditional Tumor Suppressor
<p><p>Pin1 specifically binds to and catalyzes the <i>cis-trans</i> isomerization of phosphorylated-Ser/Thr-Pro motifs, which modulate the stability, localization, and function of numerous Pin1 substrates involved in cell cycle and tumorigenesis. During cell cycle progression, the timely synthesis …
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Solid State NMR Relaxation Studies of Triosephosphate Isomerase
… in the glycolytic protein triosephosphate isomerase. 1D spectra indicated the presence of both isotropic and anisotropic phosphate populations. These states included an unbound state with an isotropic chemical shift tensor, and a protein-bound state in which the anisotropic features are …
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Overproduction of xylose isomerase in recombinant Escherichia coli
… coli. Five strains transformed with the xylose isomerase overproduction system (pRK248/pTXI-1) were compared, based on parameters of cell metabolism and inducible enzyme activity in shake flask cultures. E. coli strain LE392 (pRK248/pTXI-l) performed the best with respect to nearly all of the …
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Purification and Properties of Triosephosphate Isomerase from Selected Chlorophyta
… some of the properties of triosephosphate isomerase from two unicellular green algae 1 Ankistrodesmus Braunii and Scenedesmus acuminatus, and one filamentous green alga, Chara sp.
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Structural origins of the catalytic power of triose phosphate isomerase
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1981.
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… with tumour formation. Pin1 is a peptidyl-prolyl-isomerase with strong preference for phosphorylated Ser-Pro or Thr-Pro motives within its protein substrates. In the present thesis, evidence for the involvement of Pin1 in the maintenance of a robust SAC response is presented. Antibodies against …
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The Role of Glucose -6 -Phosphate Isomerase in Implantation in the Ferret (Mustela Putorius)
I concluded that GPI is the 60 kilodalton implantation protein. GPI is produced and secreted by ferret CL on days 6--9 of pregnancy in ferrets and is required for embryo implantation. Further study is needed to determine whether prolactin, which is also required for implantation, affects GPI …
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Investigation of Slow Dynamics in Proteins: NMR Pulse Sequence Development and Application in Triosephosphate Isomerase
… experiments to an enzyme system, triosephosphate isomerase (TIM), for studying the function of the millisecond dynamics in catalysis. Two newly developed pulse sequences, Dipolar CODEX and R-CODEX use the 13C-15N (Dipolar CODEX) and 1H-13C or 1H-15N (R-CODEX) dipolar couplings to detect dynamics. …
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Analysing the role of Protein Disulphide Isomerase in Plasmodium and targeting function to block transmission
Protein Disulphide Isomerase (PDI)-Trans has been identified as a putative target for transmission blocking interventions in Plasmodium spp. Located on the surface of the male gamete and ookinete, previous studies have demonstrated that this protein is essential for successful malaria transmission. …
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Functional and structural characterization of the mevalonate diphosphate decarboxylase and the isopentenyl diphosphate isomerase from Enterococcus faecalis
… and the other one is the isopentenyl diphosphate isomerase (IDI), which is downstream of MDD. Functional and structural studies on the mevalonate diphosphate decarboxylase from <em>Enterococcus faecalis</em> (MDDEF) have been conducted using enzymology, isothermal titration calorimetry (ITC) and …
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Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae
… to the fuel ethanol. In this study, three xylose isomerase genes originating from Haemophilus influenzae Rd K20, Arabidopsis thaliana and Bacteroides thetaiotaomicron VPI-5482 were expressed in S. cerevisiae to produce a strain capable of metabolising D-xylose (a biomass constituent). Resulting …
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NcCyP41, a two domain neurospora crassa cyclophilin : characterization of its peptidyl-prolyl cis/trans isomerase activity ; isolation and functional analysis of two novel NcCyP41-binding proteins
NcCyP41 and its interactions were characterized. Its domains were cloned in different expression vectors and recombinant proteins purified from E. coli. Interaction of NcCyP41 and its domains with cellular proteins were then analysed by biochemical methods. <br> <br>PPIase activity of the entire …
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Analysis of enzymatic L/D-peptide isomerization in animals
… results, it was hypothesized that an L/D-peptide isomerase exists in Aplysia which is responsible for the biosynthesis of DAACPs. An assay for isomerase activity was developed and used to partially purify and characterize an L/D-isomerase in the Aplysia central nervous system. Furthermore, …
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The chemistry and evolution of enzyme function: isomerases as a case study
… structural rearrangements between isomers, the isomerases (EC 5). A comprehensive analysis of the overall chemistry of isomerase reactions based on bond changes, reaction centres and substrates and products revealed that isomerase reactions are chemically diverse and difficult to classify using …
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Production of lactic acid by microbial fermentation of hemicellulose sugars
… into xylulose by the immobilized xylose isomerase which helps to promote the utilization of xylose by the L. pentosus. To facilitate the recycle and reuse of the immobilized xylose isomerase, the immobilized enzyme (65 g) was packed in a fixed bed reactor with a permeable wall. The lactic …
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Entwicklung eines GFP-Reportersystems in Legionella und molekularbiologische Funktionsanalyse des Legionella Mip-Proteins
… Funktion von PPIasen (Peptidyl-Prolyl-cis/trans-Isomerasen) wie dem Mip-Protein in Prokaryoten bis heute weitgehend unbekannt ist, sollte im zweiten Teil dieser Arbeit versucht werden, einen Interaktionspartner zu identifizieren und den Einfluss der Dimerisierung und der PPIase-Aktivität des …
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Recombinant Protein Production in Bacillus Species, Isolation and Methionine Production in Methionine-producing Microorganisms
<p>Linoleic acid isomerase (LAI) is the enzyme that converts linoleic acid to conjugated linoleic acids (CLAs) which appear to be capable of lowering cancer risk and enhancing immunity. There is an interest in developing commercial processes for the production of single isomers of CLA by …
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