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Showing 1 to 15 of 15 for “"Isocitrate lyase"”.
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Structural and mechanistic investigation towards the regulation of M.tb isocitrate lyase
Isocitrate lyase (ICL) is a crucial enzyme for the virulence and survival of Mycobacterium tuberculosis (M. tb) during infection. It is a key enzyme of the glyoxylate shunt and the methylcitrate cycle, metabolic pathways that are central to the bacteria’s carbon and lipid metabolism. The absence of …
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Novel Inhibitors for Isocitrate Lyase as a Potent Antitubercular Agent for Mycobacterium Tuberculosis
… latent TB. It has been shown that the enzyme isocitrate lyase (ICL) is essential for the survival and persistence of Mtb in latent TB. ICL plays an important role in two metabolic pathways required during TB latency, the glyoxylate and methylcitrate cycle. ICL is absent in humans, and …
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Embryonic control of isocitrate lyase activity in the megagametophyte of Ponderosa pine seeds
… presented evidence for enhancement of isocitrate lyase (threo-L<sub>S</sub> isocitrate glyoxylate lyase, EC 4.1.3.1) activity by a factor that diffuses into the megagametopnyte from the embryo of stratified germinating Pinus ponderosa seeds. In contrast, the data reported here indicate …
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Evidence for continuous potential for gene transcription during the cell cycle of a eukaryote
… potential expression of the structural gene for isocitrate lyase (three D<sub>S</sub>-Isocitrate glyoxylate-lyase, EC 4.1.3.1) during the cell cycle. Synthesis of the enzyme could be induced by placing cultures in the dark on acetate, with the induction process occurring in a quadratic fashion. …
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Three enzymes - One substrate Regulation of carbon flux through a "non-canonical" metabolic branchpoint
… this by using the “glyoxylate shunt”. Here, isocitrate is cleaved by isocitrate lyase (ICL) to yield succinate and glyoxylate (which, in a subsequent reaction, is combined with a further acetyl-CoA unit to yield the gluconeogenic precursor, malate). However, ICL has to compete with the TCA …
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Finding Typhoid Mary: Identifying Latent Carriers of Salmonella enterica serovar Typhimurium
… model. We found that aceA, a gene that codes for isocitrate lyase, is significantly upregulated on days 10 and 30 post infection. Isocitrate lyase is part of the glyloxylate cycle. Some bacterial species have developed a mechanism to utilize acetone as a carbon source to synthesize tricarboxylic …
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The Role of the Glyoxylate Cycle in the Pathogenesis of Mycobacterium tuberculosis
… isoforms of the glyoxylate cycle enzyme isocitrate lyase (ICL). The glyoxylate cycle is employed by cells when fatty acids are the main carbon source available. Here, we show that these enzymes are jointly required by M. tuberculosis for growth on fatty acids and for virulence in …
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Glyoxylate Metabolism in Mycobacterium smegmatis
… of the glyoxylate shunt of M. tuberculosis, isocitrate lyase (ICL), was shown to be required for virulence in experimental infections of mice. The other enzyme of the glyoxylate shunt, malate synthase (MLS), may also be important for the intracellular survival of the tubercle bacillus; yet, …
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Genom- und Transkriptionsanalyse von <i>Bacillus licheniformis</i> DSM13 - einem Organismus mit großem industriellem Potential
… Anwendungen, wie Proteasen, Pektat-Lyasen, Lipasen und diverse Polysaccharid-abbauende Enzyme, identifiziert. Hinsichtlich des Metabolismus erwies sich das Vorhandensein der Gene einer Isocitrate-Lyase und einer Malat-Synthase, welche die Schlüsselenzyme des Glyoxylatzyklus …
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The glyoxylate shunt as a target for antibacterial intervention in Pseudomonas aeruginosa
… glyoxylate shunt is comprised of two enzymes: isocitrate lyase and malate synthase G, and allows the synthesis of cellular constituents from C2 nutrient sources, bypassing the decarboxylation steps of the citric acid cycle when glucose is not available. The glyoxylate shunt has potential as a …
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Metabolic pathways of Campylobacter fetus
… in the cycle was supported by the reaction of isocitrate lyase which is a key enzyme of the glyoxylate bypass, and was found in the three strains. Malate synthase was not detectable in either of them, but can be induced by growing the organisms in BM + 0.5% glucose or 0.5% acetate. No pyruvate …
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Roles of the Methylcitrate and Methylmalonyl-COA Pathways in Mycobacterial Metabolism and Pathogenesis
… which, in M. tuberculosis, uses a bifunctional isocitrate lyase/methylisocitrate lyase (ICL/MCL), is one of the two routes for metabolism of propionyl-CoA. A mutant strain of M. tuberculosis lacking the ICL/MCL was rapidly cleared from the lungs of infected mice. However, the upstream enzymes of …
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Studies on lipoic acid metabolism
… three enzymes are: trace levels of OGDH, the isocitrate lyase of the glyoxylate shunt, and aspartate oxidase, the enzyme catalyzing the first step of nicotinamide biosynthesis. In chapter 4, I characterize the lipoate-protein ligase of the yeast S. cerevisiae. The E. coli LplA was the first …
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Die Nutzung der Hefe Yarrowia lipolytica zur Produktion von Citronensäure aus nachwachsenden Rohstoffen
… Die gezielte Überexpression des für die Isocitratlyase codierenden Gens ICL1 durch die Erhöhung der Kopiezahl führte zu einer drastischen Erhöhung der Enzymaktivität in den entsprechenden ICL1 multicopy Transformanden (10-15fach gegenüber Wildtyp) aufgrund des Gen-Dosis-Effektes. Auf den …