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Showing 1 to 14 of 14 for “"Iron-sulfur protein"”.
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Spectroscopic and Electrochemical Analysis of the Rieske Iron-Sulfur Protein of the Rhodobacter Sphaeroides Bc1 Complex
The Rieske iron-sulfur protein (ISP) subunit of Rhodobacter sphaeroides cytochrome bc1 complex was isolated and then investigated using molecular spectroscopies (high-resolution electron paramagnetic resonance and resonance Raman), protein-film voltammetry, and x-ray crystallography. Major …
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Ligand Binding and Structural Dynamics in C-Type Cytochromes
… related to the position of the headgroup of the iron-sulfur protein.
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Investigating pathogen 4Fe-4S protein targets and cancer chemotherapies with bisphosphonate/diphosphate inhibitors
… and ultimate enzymes of the MEP pathway, IspG (iron sulfur protein G; also known as GcpE and E-4-hydroxy-2-C-methyl-erythritol pyrophosphate synthase) and IspH (also know as LytB and E-4-hydroxy-2-C-methyl-erythritol pyrophosphate reductase), respectively. Investigation of novel cancer drugs …
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Macromolecular recognition in the cytochrome P450(cam) enzyme system
… by putidaredoxin, a Fe$\sb2$S$\sb2$ iron-sulfur protein. The mammalian cytochromes P-450 are also known to interact with cytochrome b$\sb5$, a small redox protein for which a high resolution crystal structure is available. To characterize the molecular cytochrome P-450$\sb{\rm cam}$ …
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Effect of experimental complex III deficiency on respiratory chain assembly and function
… known, of which BCS1L incorporates the Rieske iron-sulfur protein (RISP) in the last stage of the assembly. The most severe CIII deficiency, due to a mutation in BCS1L (homozygous c.232A>G), is GRACILE syndrome (growth restriction, aminoaciduria, cholestasis, iron accumulation, lactic acidosis, …
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Mössbauer and EPR studies of iron-containing proteins
"We have studied the iron-sulfur protein rubredoxin (Rd) and the heme protein horseradish peroxidase (HRP) using Mossbauer spectroscopy (MS) and electron paramagnetic resonance (EPR). Both methods are sensitive probes of the electronic structure of the iron at the active site of the protein. In all …
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Genetic Variants in the Putidaredoxin-Cytochrome P-450(cam) Electron Transfer Complex: Identification of the Residue Responsible for Redox-State Dependent Complex Stabilization
… cytochrome P-450$\sb{\rm cam},$ and a two-protein electron transfer chain, putidaredoxin and putidaredoxin reductase (Tyson et al., (1972) J Biol. Chem. 274, 5777-5784). The enzymatic removal of putidaredoxin's C-terminal tryptophan is known to cause a much reduced rate of enzymatic …
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Structural studies of metalloenzyme complexes in acetogenic carbon fixation
… that employ transition metals such as iron, nickel, and cobalt towards the production of acetyl-CoA, the final product. In one stage of the pathway, the cobalt-containing B₁₂ cofactor harbored by the corrinoid iron-sulfur protein (CFeSP) transfers a methyl group from …
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The Structure-Function Interface in the Cytochrome Bc1 Complex Family
… the conserved ligands indicating either an iron-sulfur protein (ISP), two b-hemes (cyt b) subunit, or a c-heme (cyt c) were used to study the phylogeny of each of these polypeptides. Cyt b subunit of the bc1 complex from Rhodobacter sphaeroides was modified to introduce two distinctive …
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Electron gating and mechanism of proton exit in quinol oxidation in cyt bc1 complex from R. sphaeroides
… the mobile extrinsic domain of the Rieske iron sulfur protein (ISP) with catalytic interfaces on cyt b and cyt c1 is now well established on the basis of the X-ray crystallographic structures and mutational studies, most previous discussions of mechanism have been in the context of a …
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Proteinkonjugationen durch Kupfer-abhängige Formylglycin-generierende Enzyme zur Generierung von Antikörper-Wirkstoff-Konjugaten
… utilized for the regioselective conjugation of proteins. In an oxygen-dependent reaction it catalyzes the formation of the non-canonical amino acid Cα-formylglycine (FGly) from the cysteine residue of a CxPxR-type core recognition se-quence. In addition to cysteines, the prokaryotic, anaerobic …
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Qo site of the bc1 complex; unlocking the gate of the electron transport chain
… spin of the reduced 2Fe2S cluster of the iron-sulfur protein, likely involving the pyrrole nitrogen of His 152, and possibly longer-range interaction with the oxidized b-type hemes. However specific assign-ment of bonding/quantum coupling parameters will require more detailed spectroscopy, …
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Structure, function and inhibition of GcpE, FPPS and GGPPS: targeting isoprenoid biosynthesis for drug discovery
… other have 50% of the activity seen in wild-type protein, since there is still one catalytically active AB domain. However, a plant IspG functions as an AA*B monomer and we propose using computational modeling and electron microscopy that the A* insert domain has a TIM barrel structure that …
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Protein Coevolution and Coadaptation in the Vertebrate bc1 Complex
… and three-dimensional structures of the protein complex, selection was characterized in terms of coevolution and coadaptation. Coevolution is described as reciprocal local biochemical shifts based on phylogenetic location and results in overall maintenance. Coadaptation, on the other …