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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 99 for “"Iron-Sulfur"”.
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Biosynthesis of Iron-Sulfur Clusters
… and NifU are involved in the mobilization of sulfur and iron necessary for nitrogenase-specific [Fe-S] cluster assembly. The NifS protein has been shown to have cysteine desulfurase activity and can be used to supply sulfur for the <I>in</I> <I>vitro</I> catalytic formation of [Fe-S] clusters. …
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Chemical Modification of Iron-Sulfur Cofactors
Iron–sulfur (Fe–S) cofactors are multi-metallic clusters that are found in all living organisms. The presence of multiple metal centers enables these cofactors to perform some of the most challenging chemical transformations in biosphere, such as activation of strong C–H bonds and reduction of N2, …
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Assembly of Iron-Sulfur Clusters In Vivo
Iron-sulfur [Fe-S] clusters are protein cofactors that facilitate various life-sustaining biological processes. Their in vivo assembly is accomplished by three different systems known to date. These are: the NIF system which provides [Fe-S] clusters for nitrogenase and other nitrogen-fixing …
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Biogenesis of iron-sulfur clusters and intracellular iron metabolism
Iron-sulfur ([Fe-S]) clusters represent one of natures most diverse and ubiquitous protein prosthetic groups. [Fe-S] proteins are integral for diverse biological processes. Reconstitution of apo-proteins can occur by exogenously adding excess iron and sulfide. However, due to the toxicity of iron …
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Genes from Arabidopsis involved in iron-sulfur cluster biogenesis
Iron sulfur [Fe-S] proteins are essential components of many major biological processes including electron transport, respiration, photosynthesis, hormone biosynthesis, and environmental sensing. The process of [Fe-S] cluster assembly in living cells is a controlled mechanism that is highly …
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Molecular Details Of The Mitochondrial Iron Sulfur Cluster Assembly Pathway
<p>MOLECULAR DETAILS OF THE MITOCHONDRIAL IRON SULFUR CLUSTER ASSEMBLY PATHWAY</p> <p>Iron-sulfur clusters are an important class of prosthetic group involved in electron transfer, enzyme catalysis, and regulation of gene expression. Their biosynthesis requires complex machinery located within the …
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Biogenesis and Redox Transition of Iron-sulfur Clusters in Proteins
Iron-sulfur proteins comprise one of the most ubiquitous and conserved classes of proteins in biology. The diverse functions of iron-sulfur clusters in proteins range from electron transfer to redox signaling. The assembly of iron-sulfur clusters in cells requires a complex protein system and …
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Investigations of Iron–Nitrogen Bonding at Synthetic Iron–Sulfur Clusters
… chemistry of nitrogenous ligands at high-spin iron is investigated through the synthesis and characterization of a terminal imido complex of a cuboidal tetra(μ3-sulfido)tetrairon (Fe4S4) cluster. Structural, spectroscopic, and computational studies establish a dynamic interplay between Fe–N, …
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Isolation of in vivo intermediates in iron sulfur cluster biogenesis
Iron-sulfur clusters are simple inorganic cofactors that are ubiquitous in living systems. The assembly of iron sulfur clusters is an essential process and must be carefully controlled in order to limit the release of toxic free iron or sulfide. Thus far there are three known protein systems for …
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Synthesis of Isotopically Labeled Fe and S-alkylated Iron-Sulfur Clusters
Radical S-adenosylmethionine (SAM) enzymes (RS enzymes) use a 3:1 site-differentiated [Fe₄S₄]⁺ cluster to reductively cleave the SAM cofactor and generate a 5’-deoxyadenosyl radical intermediate (5’-dAdo•) that regio- and stereospecifically abstracts an H-atom from the target substrate. It has been …
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Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis: Iron-Sulfur Biochemistry and Enzymology
The iron-sulfur prosthetic group of glutamine PRPP amidotransferase was found to be a {4Fe-4S} cluster. In the native enzyme, the cluster exists in the diamagnetic +2 redox state. Treatment of the cluster with oxidants resulted either in no reaction or in oxidative dissolution of the cluster, i.e., …
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The metabolism of iron-sulfur clusters during hydrogen peroxide stress in escherichia coli
… regulon, including genes that encode the Suf iron-sulfur-cluster assembly system. E. coli normally relies on the Isc system for cluster assembly; however, when E. coli scavenger mutants were exposed to low-grade H2O2 stress, Suf was needed to maintain dehydratase activities. Experiments showed …
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Biophysical Investigations of the Cytosolic Iron-Sulfur Cluster Assembly Pathway Late Acting Proteins
The cytosolic iron-sulfur ([Fe-S]) cluster assembly (CIA) pathway matures cytosolic and nuclear iron-sulfur dependent proteins found in eukaryotic cells. [Fe-S] clusters are involved in life-sustaining processes such as: DNA replication and repair, transcription, translation, and nucleotide and …
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Biochemical characterization of a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
The iron-sulfur flavoprotein (Isf) from the acetate utilizing methanoarchaeon Methanosarcina thermophila was heterologously produced in Escherichia coli, purified to homogeneity, and characterized to determine the properties of the iron-sulfur cluster and FMN. Chemical and spectroscopic analyses …
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Electrochemical Transformation of Alkanes, Carbon Dioxide and Protons at Iron-Porphyrins and Iron-Sulfur Clusters
… generation of hydrogen using iron-sulfur cluster. Chapter 1 gives a general overview of the electrochemical techniques which underpin the work presenedt in this thesis. Chapter 2 reports the chemical and electrocatalytic oxidation of hydrocarbons to alcohols and epoxides by …
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Structural insight into the assembly of iron-sulfur clusters and their function in radical generation
… addresses two emerging areas in the study of iron-sulfur cluster biochemistry: bioassembly of iron-sulfur clusters, and their involvement in initiation of radical chemistry. The structure of a cysteine desulfurase involved in cluster bioassembly in the cyanobacterium Synechocystis PCC sp. 6803 …
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Experimental Characterization of Iron-Sulfur Cluster Excited States and their Relevance to Electron Transfer Reactions
Iron-sulfur (Fe−S) clusters, and cuboidal [Fe₄S₄] clusters in particular, are biologically ubiquitous metallocofactors involved in a diverse array of cellular processes. The electronic structures of these metallocofactors are highly multiconfigurational, and characterized by dense manifolds of …
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Controlled Expression and Functional Analysis of the Iron-Sulfur Cluster Biosynthetic Machinery in Azotobacter vinelandii
A system was developed for the controlled expression of genes in Azotobacter vinelandii by using genomic fusions to the sucrose catabolic regulon. This system was used for the functional analysis of the A. vinelandii isc genes, whose products are involved in the maturation of [Fe-S] proteins. For …
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