Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Iron Starvation"”.
-
CHARACTERIZING THE ROLE OF PUTATIVE VIRULENCE GENES ASSOCIATED WITH INFECTION, COLONIZATION AND PERSISTENCE OF SALMONELLA ENTERITIDIS IN CHICKEN USING A BIOLUMINESCENT REPORTER
… during yolk sac infection hinted that Salmonella iron homeostasis may be critical to pathogenesis in a chicken model. The contribution of various iron uptake systems has been investigated comprehensively in murine models with inconsistent data. Lack of information in a chicken model encouraged us …
-
Rgg2/Rgg3 Quorum Sensing in Streptococcus pyogenes and Innate Immune Cell Responses
… of nitric oxide generates dinitrosyliron complexes which deplete the available intracellular iron in S. pyogenes and induces iron starvation. These results give more context to the functions of dinitrosyliron complexes in biological systems which previously were poorly understood. In …
-
The Role of Compartmentalized Metabolism in Cellular Metal Homeostasis
… life, as we know it, is not possible without iron. While the list of iron's vital cellular functions is extensive, iron is also quite cytotoxic. Thus, great pains have been taken, at the gene regulation level, to assure that a cell has sufficient iron to copy its genome and power its …
-
FUNCTIONAL STUDIES OF THE FE-S CLUSTER BIOGENESIS BY SUF SYSTEM
Iron-Sulfur clusters are important metal cofactors for many enzymes. Bacteria have dedicated systems for the maturation and trafficking of Fe-S clusters. The SUF (Sulfur Utilization Factor) system is the only housekeeping system in Gram-positive bacteria; whereas in Gram-negative bacteria, the SUF …
-
Characterization of The Rna Binding and Rna Degrading Subunits of The Eukaryotic Exosome
… for the exosome and that in its absence the iron starvation response of the cell is activated.</p> <p>Despite numerous studies of the exosome, only a few address the physiological relevance of the catalytic activity. This work shows that the exonuclease of the exosome is important for …
-
Characterization of ferredoxin and flavodoxin as molecular indicators of iron limitation in marine eukaryotic phytoplankton
… to assess their utility as biomarkers of iron limitation. A phylogenetic survey of seventeen microalgal species showed flavodoxin induction, with accompanying ferredoxin repression, to be a common response to iron stress. A minority of organisms examined never expressed flavodoxin, a …
-
Characterization of Fe-S Cluster Assembly On the Sufbc2D Complex
<p>In vivo biogenesis of iron-sulfur (Fe-S) cluster cofactors requires complex biosynthetic machinery to limit release of iron and sulfide, to protect the Fe-S cluster from oxidation, and to target the Fe-S cluster to the correct apo-enzyme. The SufABCDSE pathway for Fe-S cluster assembly in E. …
-
A genomic analysis using RNA-Seq to investigate the adaptation of the psychrophilic diatom Fragilariopsis cylindrus to the polar environment
… F. cylindrus has evolved to cope with marine environmental conditions of the Southern Ocean. To identify key genes, comparative genomics, high-throughput transcriptome sequencing and reverse genetics were applied. Comparative genomics with the sequenced mesophilic diatoms Phaeodactylum …
-
Secondary problems caused by the oxyr response to hydrogen peroxide stress in Escherichia coli
… (H2O2) diffuses into cells, where it reacts with iron via Fenton chemistry. Three cellular targets are damaged: DNA, mononuclear iron enzymes, and [4Fe-4S] cluster enzymes. E. coli uses the transcription factor OxyR to sense and respond to H2O2 stress. When the intracellular H2O2 levels rise to …
-
Characterizing the Roles of Sufb and Sufa in Fe-S Cluster Assembly and Trafficking in Suf Pathway
<p>Iron-sulfur (Fe-S) clusters are essential cofactors in various metalloproteins and are best known for their roles in electron transport as well as redox and non-redox catalysis. Three Fe-S cluster assembly systems: Nif, Isc, and Suf have been identified. The sufABCDSE operon is activated in E. …
-
Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
… stress conditions including, but not limited to, iron starvation, carbon flux and metabolism, virulence, and quorum sensing. In this study, we investigate the multitude of clever mechanisms that two sRNAs, SgrS and DicF, utilize to regulate gene expression, and the physiological consequences of …
-
Investigations of conserved structure-function relationships and enzymatic evolution
The E.coli SUF pathway is the iron-sulfur cluster biogenesis pathway responsible for assembling iron-sulfur clusters during times of oxidative stress and iron starvation. SufS is the cysteine desulfurase enzyme responsible for the acquisition of sulfur and subsequent transfer to transpersulfurase …
-
Investigating Iron Transport and Utilization Features of Acinetobacter baumannii
… infections. The bacterium must overcome iron starvation and oxidative stress conditions imposed by the host in order to propagate and cause disease. This work further investigates the transport and utilization of iron by <i>A. baumannii</i>, and their involvement in virulence. The …
-
Assembly of Iron-Sulfur Clusters In Vivo
Iron-sulfur [Fe-S] clusters are protein cofactors that facilitate various life-sustaining biological processes. Their in vivo assembly is accomplished by three different systems known to date. These are: the NIF system which provides [Fe-S] clusters for nitrogenase and other nitrogen-fixing …