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Showing 1 to 20 of 95 for “"Ion exchange chromatography"”.
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Studies in ion exchange chromatography
This dissertation investigates three major problems in IC, ranging from the practical to the theoretical. The first is of interest to the field of quantitative analysis. Since ion chromatography is primarily a quantitative technique, the linear range of the detector is of particular interest. The …
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Proteomics approach to investigate separation behavior of proteins in ion-exchange chromatography
… point (pI), molecular weight (MW) and partitioning coefficient (K) were characterized for a mixture of proteins using a three-dimensional (3D) method which combined hydrophobic partitioning with two-dimensional (2D) electrophoresis. Protein pI and MW were obtained from 2D gels directly using …
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Analysis of the Structural Units of Sulfated Glycosaminoglycans by High Performance Ion Exchange Chromatography
… of heparin produced by nitrous acid deamination were stoichiometrically reduced with NaB('3)H(,4) and analyzed by high performance ion exchange chromatography to obtain resolution of the four primary disaccharide units of heparin; 4-O-((beta)-D-glucopyranosyluronic …
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Macroscopic continuum and molecular dynamics modeling and simulation studies of the transport and adsorption of charged macromolecules in ion-exchange chromatography systems
… continuum rigorous model of the conditions necessary for the development of an overshoot in the concentration of a charged bioactive macromolecule in the adsorbed phase of charged adsorbent particles is presented, and the parametric sensitivity of the concentration overshoot phenomenon …
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Arsenic speciation by ion-exchange chromatography with on- line determination by hydride generation: electronic modification of the D.C. plasma by photon counting
A method using L-cysteine for the determination of arsenous acid (As(III)), arsenic acid (As(V)), monomethylarsonic acid (MMAA), and dimethylarsinic acid (DMAA) by hydride generation was demonstrated. The instrument used was a d.c. plasma atomic emission spectrometer (OCP-AES). Complete recovery …
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Isolation of immunoglobulins from cheese whey and milk using ultrafiltration and immobilized metal affinity chromatography
… whey was concentrated using an ultrafiltration (OF) membrane with a 50 kDa molecular weight cut-off (MWCO). The concentrated whey was applied to an immobilized metal affinity chromatography (IMAC) column to isolate IgG. With a 50 ml IMAC column loaded with 17.3 ml of 50 mM CuCl₂ to get 2/3 …
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Methionine: oxidation state in processed foods and enzyme-catalyzed reaction with adenosine triphosphate
Two conditions of alkaline hydrolysis of proteins, (1) 2M NaOH, 18 hours, 100°C and (2) 3M NaOH, 16 hours 110°C, prior to ion-exchange chromatography were tested on free amino acids and model protein systems to determine the better set of conditions for measurement of methionine sulfoxide in food …
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Cryptosporidium Parvum Microbial Adhesion: Interactions Between Sporozoites and Host Cells
… about the mechanisms of attachment and invasion of C. parvum sporozoites to host cells, our goal was to elucidate the mechanism of sporozoite recognition of receptors on the host cell membrane. Knowledge of these mechanisms may allow for novel therapeutic strategies for cryptosporidiosis. An …
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ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΚΙΝΑΣΗΣ ΚΑΖΕΙΝΗΣ ΙΙ ΑΠΟ ΣΠΛΗΝΑ ΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ
… THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME PHOSPHORYLATES IN PREFERENCE ACIDIC PROTEINS, CASEIN, USING ATP AND GTP AS PHOSPHATEDONORS, IN THE PRESENCE OF MGCL2. THE …
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Synthesis of Labeled 3-Hydroxyproline and Biosynthesis of the Dehydroproline Moiety of Virginiamycin M₁
… from S-[carboxyl-¹⁴C] proline. The oxygen functionality at the three position was obtained by acetylation of 1,2-dehydroproline methyl ester using lead tetraacetate. Reduction of the imine with sodium borohydride gave predominately (R,S)-[caboxyl-¹⁴C]-cis-3-acetoxyproline which was hydrolyzed …
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THE DETERMINATION OF TRACE METALS IN CONCENTRATED BRINES FOR PROCESS CONTROL
The determination of the alkaline earths in concentrated feed brines is required for process control purposes in the chlor-alkali industry. Methodology was developed, based on ion-exchange chromatography, for the determination of alkaline earths in 30% sodium chloride. Brine samples were loaded …
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Fasting Plasma Amino Acids in Relation to Serum Lipids in Human Male Subjects
<p>The association of fasting plasma amino acids with serum lipids was studied in 23 healthy human male subjects with ages ranging from 31 to 62 years (45±9, mean± SD). There were 12 normocholesterolemic (serum cholesterol = 183±17 mg/dL) and 11 hypercholesterolemic (271±27 mg/dL) subjects. Venous …
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Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni
Isolation, cloning and sequencing of the cDNAs encoding for the toothfish crystallins revealed a total of 21 isoforms (2 alphas, 6 betas, and 13 gammas). Phylogenetic analyses suggest that toothfish alpha and beta crystallins are orthologues of mammalian counterparts, while gammas do not. Further …
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Amino Acid Sequence Studies on the Beta Subunit of Bacterial Luciferase
… acid sequences were determined by Edman degradation. The protein was fragmented by digestion with S. aureus V8 protease, trypsin, trypsin after citraconylation, and by treatment with cyanogen bromide. A substantial proportion of each peptide mixture precipitated and was not amenable to …
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Determination of L- and D-methionine and L- and D-valine in soy isolate and soy flour samples
L to D isomerization of amino acids in a commercially prepared alkali-treated food-grade soy isolate was investigated. A soy flour product was also investigated as a control which had not been alkali treated. Methionine and valine collected from two soy flour and two soy isolate samples were …
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Purification and characterisation of amylolytic enzymes from Lipomyces starkeyi
A purification scheme has been developed for the extracellular carbohydrases; aglucosidase and a-amylase, secreted by the ascosporogenous soil yeast Lipomyces starkeyi NCYC 1436. Growth and optimum enzyme yield conditions were determined with cultures of L. starkeyi grown at 30°C on a medium …
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Studies on biotin as a coenzyme of propionyl carboxylase
… powder which catalyzes the synthesis of propionyl holocarboxylase from d-biotin and its apocarboxylase, in the presence of ATP and Mg++ ions. The enzyme system has been partially purified by (NH₄)₂SO₄ precipitation and resolved into two obligatory components by alumina C y gel fractionation. …
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Structural analysis of glycolipid-derived oligosaccharides from metabolically radiolabelled colorectal carcinoma SW1116 cells
This dissertation describes the analysis of the carbohydrate portion of glycosphingolipids from colorectal carcinoma cells, SW1116, by metabolically labelling the cells with radioactive monosaccharide precursors. SW1116 cells (1 x 10⁶) metabolically labelled with 222 μCi/ml of either …
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LECTINS FROM THE HORSESHOE CRAB, LIMULUS POLYPHEMUS, REACTIVE WITH BACTERIAL LIPOPOLYSACCHARIDE
… R mutants of Salmonella minnesota. Precipitation-inhibition studies using 2-keto-3-deoxyoctonate (KDO) have supported the hypothesis that this acidic residue is the immunodeterminant group in the core region of the lipopolysaccharide molecule. Limulus also contains a lectin which interacts …
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New technique for radiolabelling tracer with 64CU for positron emission particles tracking (PEPT) experiments
Positron emission particle tracking (PEPT) is a non-invasive technique for studying the flow of particulate systems within industrial equipment. The technique tracks a tracer particle labelled with a positron emitting radionuclide moving within the field-of-view of a positron emission tomography …
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