Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Iodixanol"”.
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Development of a multi-stage purification process for serum-derived exosomes and evaluation of their regenerative capacity
… prior to SEC that incorporated a high-density iodixanol cushion overlaid by a 18% iodixanol step containing UC concentrated human serum that was then overlaid with 6% iodixanol. This DG relied on flotation to remove lipoproteins. After the multi-step purification (UC DG SEC) ApoB and ApoA1 were …
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Are gadolinium contrast media really less nephrotoxic than iodine agents in radiographic examinations? A comparison in relation to their ability to attenuate x-rays in a pig model
… 1.0M gadobutrol (1.6 Osm/kg), 0.5M gadodiamide, iodixanol 150 and 320 mg I/mL (290 mOsm/kg) and iopromide 150 mg I/mL (340 mOsm/kg). The plasma half-life elimination time of a GFR-marker were used to compare their effects on glomerular filtration rate 1-3 hours post-injection. III) After the …
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Optimization and characterisation of plant produced Human Papillomavirus pseudovirions in Nicotiana benthamiana
… L2 capsid proteins. Particles were purified by iodixanol density gradient ultracentrifugation and the 27% and 33% fractions of this gradient analysed. Transmission electron microscopy (TEM) was used to confirm particle assembly and L1 expression was quantified by ELISA. Particles were disrupted …
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Interrogations of protein and RNA: localisation, interactions and dynamics
… fractionation procedure using multiple iodixanol containing gradients, termed “MultiLOPIT”, is presented, which provides high-resolution organelle resolution for comparative applications in different cell states or conditions. In Chapter 4, characterisation of the dynamics of protein and …
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Increasing the optical transparency of a living mouse brain (and other nanotechnologies)
… including the iodinated radiocontrast agent iodixanol, high molecular weight polyethylene glycol (PEG), high molecular weight Dextran, and PEG-ylated Silicon nanoparticles. We achieve ~2x increases in the brightness of cells expressing red fluorescent proteins in vivo in mice, as measured by …
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Development of a methodology for applying shear stress to suspended mammalian cells
… cell density at a precision of 0.002 g/ml with iodixanol under an inverted microscope. A neutrally buoyant single-cell suspension recipe is tested. Cells cultured within the broth are not only capable of remaining suitable for long term shear exposure, but also presented enhanced health …
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Working towards a plant-produced Bovine papillomavirus vaccine: expression of BPV1 virus-like particles and pseudovirions in Nicotiana benthamiana
… centrifugation of clarified plant extract on an iodixanol density gradient prepared with a 3M NaCl buffer. PsVs produced through these methods were shown to be capable of pseudo-infecting and expressing the reporter gene in mammalian HEK293TT cells, and neutralisation of the PsVs by different …