Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Inverse PCR"”.
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Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques
… temperature polymerase chain reaction (COLD-PCR), inverse-PCR and Pippin Prep™ gel electrophoresis, were analysed to identify a novel approach to selectively enrich shorter cffDNA fragments from larger maternal cell-free DNA (cfDNA). The sensitivity of droplet digital PCR (ddPCR) against …
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Escherichia coli mutants unable to use a combination of L-serine, glycine and L-leucine as carbon source
… in the activation of L-SD enzyme. Applying inverse PCR technique, I identified the mutated gene in these two mutant strains to be nuoM and torA respectively. A formerly isolated SGL- mutant MEW84 was also identified as a $\lambda$placMu insertion in the glpC gene. Further experiments proved …
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Association of <i>glpQ</i> with serum-resistance in <i>salmonella typhimurium</i>
… insertion and reduced serum-resistance. Inverse PCR and subsequent DNA sequence analysis reveal the insertion to be in the gene <em>glpQ</em>. <em>glpQ</em> codes for a periplasmic glycerophosphodiester phosphodiesterase involved in glycerol degradation. Complementation assays using a …
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Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052
… a plasmid integrated into the scrB gene, and inverse-PCR respectively. The four genes of the scrARBK operon were prowsed to encode an Enzyme1IBC1ucrose protein of the PTS (ScrA); a transcriptional repressor of the GalR-Lacl family (ScrR), a sucrose-6-phosphate hydrolase of the glucosyl …
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Cytokine modulations by type I interferon suppression-negative and NF-κB activation-negative Porcine Reproductive and Respiratory Syndrome Virus
… from PRRSV by reverse genetics and extralong inverse PCR, and mutant viruses were generated: type I IFN suppression-negative PRRSV (P1β-∆135), NF-κB activation-negative PRRSV (PN-∆NLS), and both type I IFN suppression-negative and NF-κB activation-negative PRRSV (PD-∆135-NLS). These mutant …
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Taraxacum officinale as an expression system for recombinant proteins : molecular cloning and functional analysis of the genes encoding the major latex proteins
… (PPO). Based on these peptide fragments, an inverse PCR strategy was developed to amplify a unique sequence, encoding the latex PPO. RT-PCR was used to confirm the expression of the latex ppo gene and revealed that two closely related genes encoding a PPO are expressed in the latex. …
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The relationship between fruit color traits and anthocyanin genes in diploid Fragaria
… a diploid (2n = 2x = 14) strawberry. Using PCR with degenerate primer pairs, intron-containing segments of structural genes coding for the enzymes: chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanindin synthase …
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Phosphofructokinases from extremely thermophilic microorganisms
… of the pfp gene was obtained using degenerate PCR and inverse-PCR. Sequence analysis and a phylogenetic comparison suggest that the Dictyoglomus PPᵢ-PFK represents an ancient lineage and is closely related to those PPᵢPFKѕ from the archaeal Thermoproteus tenax, bacterial Mycobacterium …
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A Mosquito DNA Transposon Agh1: Structure, Evolution and Evidence of Activity
… species that are only found in West Africa. PCR and sequencing results showed more than 99% sequence identity between AGH1 sequences in A. gambiae, A. arabiensis, and A. melas, which may indicate either purifying selection or recent horizontal transfer. To assess whether AGH1 is currently …