Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Intrinsically disordered regions"”.
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Characterizing Multivalent Interactions Between Folded Protein Domains and Intrinsically Disordered Regions or Peptide Substrates
… on interactions between structured domains and intrinsically disordered regions or peptide substrates.The subject of Chapter two is prolyl isomerase Ess1, which is an essential enzyme found in Saccharomyces cerevisiae. Ess1 regulates the transcription and co-transcriptional RNA processing by …
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Biomolecular condensation of intrinsically disordered regions in P. falciparum and H. sapiens transcription factors
… control. Biomolecular condensation, driven by intrinsically disordered regions (IDRs) in proteins, has emerged as a previously underappreciated mechanism of cellular compartmentalisation and regulation. The study of eukaryotic transcription has been revolutionised by the understanding that …
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Characterization of the Roles of Intrinsically Disordered Regions from RNA-Binding Proteins in Phase Separation
… enrich RNA and RNA-binding proteins containing intrinsically disordered regions (IDRs). I demonstrate that IDRs, coupled with RNA binding domain and RNA, can phase separate in vitro, producing dynamic liquid droplets. Over time, these droplets mature into more stable states, as assessed by …
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Investigating DNA remodeling in DNA mismatch repair
… DNA. In this thesis, we also investigate how the intrinsically disordered regions in Mlh1-Pms1/PMS2 contribute to activation and recycling of the endonuclease during DNA mismatch repair. Using previously identified point mutations within the intrinsically disordered region of yeast Mlh1 (R401A, …
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Intrinsically disordered proteins in molecular recognition and structural proteomics
Intrinsically disordered proteins (IDPs) are abundant in nature, being more prevalent in the proteomes of eukaryotes than those of bacteria or archaea. As introduced in Chapter I, these proteins, or portions of these proteins, lack stable equilibrium structures and instead have dynamic …
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Studies of the Molecular Features of Brd4-Nut and P300 That Contribute to Condensate Formation and Transcriptional Regulation
… profile similar to Brd4-Nut(MIN). The intrinsically disordered regions, transcription factor - binding domains, and HAT activity of p300 all collectively contribute to condensate formation. Conversely, only HAT activity appears to be necessary to mimic the transcriptional profile of …
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Targeting and Manipulating Endogenous Transcriptional Condensates
… transcriptional condensates. We find that intrinsically disordered regions of transcription factors can be used to direct cargo to the condensates. Combined with an improved light-induced dimer and its binding partner allow us to generate a quick response with high insertion efficiency. We …
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Biophysical mechanisms of intrinsic protein disorder: lessons from a protein backbone model
Intrinsically disordered regions (IDRs) in proteins populate a dynamic, heterogenous ensemble of structures. Proteins involved in the regulation of critical cellular functions rely on IDRs to carry out their functions. IDRs have been implicated in a number of diseases for which there are currently …
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How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH
… SLiMs are stretches of 3-10 residues found in intrinsically disordered regions of proteins. Given their degeneracy, short length, and weak affinities, it is not immediately clear how such low complexity interaction elements are able to discriminate amongst the many homologous SLiM-binding …
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Molecular modelling of Androglobin
… algorithms with the aims of identifying intrinsically disordered regions (IDRs), that may not only add noise when subjecting the sequence to fold recognition techniques. These IDRs may also be in close proximity to key molecular recognition features (MoRFs). Secondary structure prediction …
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Monitoring the Effects of the Oncogenic S250F Mutation on the Intrinsically Disordered C-terminal of FoxA1
… domain (DBD) that is flanked by two large intrinsically disordered regions (IDR). The C-terminal IDR is thought to play an important role for the pioneering activity of FoxA1. Mutations in FoxA1 have been strongly correlated with certain cancers, particularly breast cancer. A mutational …
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Sequence-Based Properties that Identify Intrinsically Disordered Phase-Separating Protein Regions
… drive liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs). This phenomenon is critical in many cellular processes (including RNA metabolism, chromatin rearrangement, and signal transduction), and known to be driven primarily, but not exclusively, by IDPs. To fully …
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Elaboration of the Homer1 Recognition Landscape Reveals Incomplete Divergence of Paralogous EVH1 Domains
… that many proteins contain unstructured regions that are necessary for the proper function of their encompassing proteins. Some of these intrinsically disordered regions are responsible for mediating protein-protein interactions that are critical for cellular processes such as protein …
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Primary Structure and Solution Conditions Determine Conformational Ensemble Properties of Intrinsically Disordered Proteins
<p>Intrinsically disordered proteins (IDPs) are a class of proteins that do not exhibit well-defined three-dimensional structures. The absence of structure is intrinsic to their amino acid sequences, which are characterized by low hydrophobicity and high net charge per residue compared to folded …
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THE ROLE OF TRANSCRIPTION FACTOR DYNAMICS IN GENE EXPRESSION: DOES TIME MATTER?
… due to protein-protein interactions mediated by intrinsically disordered regions of the TF and potentially controls the initiation rate of transcription. Finally, I studied transcriptional regulation on substrates of different stiffness revealing a connection between the physical properties of …
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The interactions of the cucumber mosaic virus 2b protein with the viral 1a and host Argonaute 1 proteins
… and in the process of investigating potential regions of the 2b protein needed for 2b-AGO1 complex formation I found evidence that the 2b protein may contain intrinsically disordered regions. Such disorder may explain the ability of the 2b protein to interact with a wide range of factors.
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Functional Characterization of Plasmodium falciparum TATA-box binding-like Protein (PfTLP)
… Secondly, PfTLP possesses two low-complexity or intrinsically disordered regions (LCR1 and 2), which are absent in TLPs from model eukaryotes. These regions are located at the same positions within the two quasi-symmetrical repeats of the TLP core structure and show a non-random compositional …
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Investigating the regulation of processing bodies in Drosophila melanogaster oocytes
… assembly. At the molecular level, although the intrinsically disordered regions (IDRs) of a core P body protein are dispensable for condensate assembly, they are critical for maintaining P body integrity in the mature oocytes. In addition, RNA is also found to be essential for sustaining P body …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… to other reported cellular forces, 2) the intrinsically disordered regions (IDRs) are dispensable for condensate formation but necessary for high force generation, and lastly, 3) chromatin components, such as nucleosomes and linker histone H1, attenuate the force generating capacity of Sox2 …
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New experimental and theoretical tools for studying protein systems with elements of structural disorder
<p>Disordered proteins are one class of proteins which do not possess well-folded three-dimensional structures as their native conformations. Many eukaryotic proteins have been found to be fully disordered or contain certain disordered regions. Disordered proteins usually display several …
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