Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Intravital imaging"”.
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Intravital imaging of metastasis in zebrafish
… These events are dynamic and so require intravital imaging to investigate. However, the intravital imaging of these events in mice is challenging. Sites of metastasis are often in vital organs that are inaccessible to microscopy without surgical intervention. Furthermore, circulating …
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Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation
… responding to 3000J/m2 UVB in the skin. Using intravital confocal microscopy and genetically manipulated transgenic mice, we have found that neutrophils are recruited to inflammatory sites immediately after UVB irradiation. Skin-infiltrating neutrophils can interact with LCs upon activation and …
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Multimodal intravital imaging of tissue structure and cell dynamics in skin using integrated optical coherence and multiphoton microscopy
… and application of multimodal optical imaging methods for providing enhanced visualization of living skin. In vivo imaging of skin is of great interest for research applications to study fundamental aspects of skin biology as well as for clinical applications to potentially enable …
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Unraveling the Effects of Nicotine-Derived Nitrosamine Ketone (NNK) on Healthy and Neurodegenerative Brain via Intravital Imaging and Multiscale Ex Vivo Imaging Approaches.
… the use of a transgenic mouse (CX3CR-1GFP) with intravital neuroimaging. We show that NNK given intranasally over acute (4-7days) and chronic (12 weeks) exposures, leads to disruption of the BBB and heterogenous microglia activation. Machine learning algorithm was employed to segment microglial …
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Identification of intravasation sites for metastatic biomarker development
… coupled with fluorescent streptavidin for intravital imaging. Combining these components, we were successful in performing intravital imaging to identify differentially labelled blood vessels, indicating possible intravasation sites. Future work will involve confirmation of the specificity …
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Mechanisms of Edema Clearance: The Central Role of Lymphatic Vessels and LYVE1 in Fluid Homeostasis
… edema. We utilized its optical transparency and intravital imaging capabilities to characterize the dynamic lymphatic responses during edema formation and resolution. Using this platform, we identified key structural and molecular changes that accompany compensatory lymphangiogenesis. …
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Imaging Biomarkers of Response to Immune Checkpoint Inhibition in Melanoma
… approach. The development of non-invasive imaging methods and imaging biomarkers of response to immune checkpoint inhibitors could therefore provide important diagnostic and prognostic information. This project builds on existing technologies in Cambridge to develop structural and …
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Macrophage-mediated resistance mechanisms against MAPK inhibitory by cancer therapeutics
… signaling networks, we develop an intravital imaging strategy to monitor the influence of tumor-associated macrophages on cancer cell kinase activity dynamics. Finally, we rationally design a nanotherapy to exploit inhibitor-induced immunomodulation and crosstalk. Overall, we …
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The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
… rates to anabolic loading. We also used intravital imaging via two-photon fluorescent microscopy to visualize the Ca2+ dynamics in osteocytes during active mechanical loading of the bone in living mice. Finally, we explore the ability of light-sheet fluorescent microscopy to create high …
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Peptide-Functionalized Layer-by-Layer Nanoparticles Demonstrate Improved Blood Brain Barrier Permeability for Glioblastoma Treatment
… were screened in a mouse model through intravital imaging, leading to a conclusion that the combination of a poly-L-aspartate outer layer and Angiopep-2 leads to significant improvement in nanoparticle uptake in the brain. Future work will include in vivo experiments with …
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Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model
… flow cytometry, and confocal microscopy-based intravital imaging allowed me to observe that extravasating prostate cancer cells exhibited significant cell volume reduction. This reduction is suggestive of an invasive cell phenotype. However, cell volume reduction at certain threshold also …
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Exploring antiviral effector cells in vaccinia virus-infected tissues
… and T cells are recruited to infected tissue. Intravital imaging revealed that group 1 ILCs continuously patrol the epithelium in both naïve and infected tissues and are the major immune force limiting oral mucosal viral infection at early timepoints. Unexpectedly, group 1 ILCs produce …
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Cellular and molecular mechanisms of liver regeneration
… parallel, a novel experimental system to permit intravital multiphoton microscopy of the regenerating liver following partial hepatectomy in mice was developed and validated. Intravital imaging of mouse liver was performed with a range of cellular labels, combined with a fluorescent cell cycle …
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Visualizing The Dynamics of Immune Surveillance In Brain Tumors By Intravital Multiphoton Microscopy
… of immune surveillance in brain tumors by intravital multiphoton microscopy</strong></p> <p>By</p> <p>Felix I. Nwajei, MD</p> <p>Supervisory Professor: Tomasz Zal, Ph.D.</p> <p>Brain tumors (BTs) generally have a bad prognosis despite conventional treatment strategies. Immunotherapy is a …
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Multiphoton microscopy for stain-free slide-free histopathology
… conventional methods for label-free multiphoton imaging are burdened by limited contrast and efficiency of individual modalities as well as complex laser systems, which hinders this technology from wider application in biomedicine. This thesis developed a fiber-based single-excitation multiphoton …