Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Intracytoplasmic sperm injection"”.
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Dynamic study of the piezo-driven pipettes in intracytoplasmic sperm injection
… THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Intracytoplasmic Sperm Injection (ICSI) is regarded as a critical assisted reproductive technology (ART) procedure in which a single spermatozoon is mechanically injected into the cytoplasm of an oocyte using an injection pipette. During ICSI, one …
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Sex Chromosome Ratios and Aneuploidy Rates in the Zona Pellucida Selected Spermatozoa From Normal and Subfertile Men
… human hemizona has been demonstrated to select spermatozoa with good motility, normal morphology, and the capacity to undergo the zona-induced acrosomal reaction. The studies conducted here are directed at using the human hemizona to investigate two key questions: (1) whether human X-chromosome …
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The association between sperm aneuploidy and male infertility: Screening, aetiology and possible routes to alternative therapy
… association between infertility and increased sperm aneuploidy. Specific questions are: should males be screened for sperm aneuploidy before intracytoplasmic sperm injection (ICSI)? Is there a relationship between individual semen parameters and sperm aneuploidy for specific chromosome pairs? …
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DNA methylation in the early porcine embryo
… technologies such as in vitro fertilization, intracytoplasmic sperm injection, parthenogenetic activation, and somatic cell nuclear transfer are powerful procedures in the production of animals for agriculture, basic research, and biomedical research. Research using these techniques has …
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Evidence based investigation and treatment of disorders of female reproduction
… and In Vitro Fertilization (IVF) with or without Intracytoplasmic Sperm Injection (ICSI). The published work on PCOS consists of treating both the infertility and long term effects of PCOS. Particular emphasis has been placed on comparing both the efficacy and adverse effects profile of the new …
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Developing embryo technologies for the eland antelope (Taurotragus oryx)
… matured to metaphase II. In vitro fertilization, intracytoplasmic sperm injection and nuclear transfer resulted in embryonic development. In conclusion, the bovine embryo culture study suggests that the beneficial effects of amino acids are influenced by the base medium and glucose plays more …
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CatSper: Characterisation in Human Spermatozoa and Clinical Significance
Before a mature spermatozoon is able to fertilise the oocyte, it must first traverse the female reproductive tract, through obstacles such as highly viscous mucus. As the sperm is thought to be transcriptionally and translationally inactive, it must rely on cues from its external environment in …
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Sexual Dimorphism in Susceptibility to Age-Dependent Metabolic Disorders in Mice Born through ICSI
Intracytoplasmic sperm injection (ICSI), as an assisted reproductive technology (ART), has been increasingly used in human fertility clinics to treat male infertility and achieve pregnancy. Consequently, the majority of the so-called "test-tube babies" are born through ICSI nowadays. Many studies …
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Meiotic defects in infertile men
While the introduction of intracytoplasmic sperm injection (ICSI) has revolutionized the treatment of male infertility, concerns have been raised regarding the risk of chromosomal abnormalities in pregnancies derived from ICSI. Studies on sperm from infertile men have suggested that this population …
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Νευραναπτυξιακές διαταραχές στην υποβοηθούμενη αναπαραγωγή
… η ενδοκυτταροπλασματική έγχυση σπερματοζωαρίου (intracytoplasmic sperm injection, ICSI) και ο προεμφυτευτικός γενετικός έλεγχος (preimplantation genetic testing, PGT), έχουν συμβάλει σημαντικά στην αύξηση των ποσοστών επιτυχούς σύλληψης και γέννησης υγιών νεογνών. Παρά την ευρεία εφαρμογή τους, …
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Aneuploidy and DNA fragmentation in morphologically abnormal sperm
Introduction: Intracytoplasmic sperm injection (ICSI) has been a successful assisted reproductive technique for men with severe male-factor infertility. However, ICSI requires the subjective selection of normal looking sperm, which does not preclude the transmission of genetically abnormal sperm. …
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Organization of Human Sperm Chromosomes During Pronuclei Formation
… have demonstrated that chromosomes of human spermatozoa have well-defined spatial organization that includes preferred intranuclear positioning. The hypothesis of this dissertation is that sperm chromosome architecture participates in controlled transformation and activation of the paternal …
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Studies on genetic aberrations as possible predictors of the outcome of assisted reproduction
… the World Health Organization (WHO) in regard to sperm concentration, motility and morphology are, however, poor predictors of fertility. In about half of the involuntarily childless couples the causes are solely or partly male related. In 60-75% of cases the aetiology of reduced semen quality …
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Numerical Chromosome Abnormalities in Sperm From Oligoasthenoteratozoospermic Patients and Fertile Males
… is the potential importance of aneuploidy in sperm. To more clearly understand the cytogenetic make-up of sperm from OAT patients, multi-probe, multi-color FISH was performed to determine aneuploidy.</p> <p>The introduction of intracytoplasmic sperm injection (ICSI) has revolutionized …
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The effect of sperm apoptosis, Reactive Oxygen Species (ROS) and semen sample handling on sperm parameters and Assisted Reproductive Technology (ART) outcomes
… to the potential sources and clinical impact of sperm apoptosis and reactive oxygen species (ROS) in the context of assisted reproductive technology (ART). This thesis encompasses three independent studies, each of which addresses a single aim related to different aspects of male infertility and …
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Assistierte Reproduktion beim Pferd
… transfer (GIFT) involves transfer of oocytes and spermatozoa into the oviduct of a recipient mare. Since only low sperm numbers are required, GIFT can be used for subfertile stallions, frozen-thawed semen and sexed spermatozoa. Conventional methods or vitrification can be applied for the …
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The Effect of Growth Hormone on Pig Embryo Development in Vitro and an Evaluation of Sperm-Mediated Gene Transfer in the Pig
… and oocytes were co-incubated with frozen-thawed spermatozoa for 8 h. Putative embryos were transferred to NCSU 23 containing 0.4% BSA and cultured for 144 h. Embryo development was assessed on d 6 of culture. The treatment groups were as follows: treatment 1 = control group cultured in IVM medium …
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Oxidative stress mechanisms within the developing porcine oocyte and the effects of antioxidant supplementation
… was analyzed at 48 h and 144 h after IVF or intracytoplasmic sperm injection (ICSI). Supplementation of antioxidants had no effect on intracellular levels of GSH, nuclear maturation or fertilization traits. Blastocyst formation for NAC (35.0 ± 7.4%) and NACA (40.0 ± 7.4%) supplementation were …
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Predictive role of fresh and post-selection total motile sperm count in the success of autologous and oocyte-donor ICSI cycles [Ruolo predittivo del numero totale di spermatozoi mobili su campione fresco e post-selezione nel successo dei cicli ICSI autologhi e con ovodonazione]
… sensibili. In questo contesto, il Total Motile Sperm Count (TMSC), che integra volume seminale, concentrazione e motilità, è stato proposto come predittore più affidabile della potenzialità riproduttiva maschile rispetto ai singoli parametri convenzionali. Questo studio multicentrico, …