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Showing 1 to 20 of 42 for “"Intracellular pH"”.
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Regulation of Intracellular pH and Plasma Membrane Potential in Trypanosoma Cruzi and Trypanosoma Brucei
Regulation of intracellular pH (pHi) and the plasma membrane potential (DeltaPsi) was investigate in all stages of the pathogenic protozoans, T. cruzi and T. brucei utilizing the pH sensitive fluorescent dye BCECF and the potentiometric fluorescent dye bisoxonol. pH i in T. cruzi was found to be …
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Intracellular pH, the Proximate Signal for Cell Volume Changes that are Mediated by the Actin Cytoskeleton
The relationship between initial intracellular pH (pHi) and associated cell volume change was investigated by simultaneous measurement of pHi and cell volume with fluorescence imaging in polarized fungiform taste receptor cells (TRCs) loaded with BCECF in vitro. Ammonium pulses caused a brief, …
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The production of 4-adminobutyrate in response to treatments reducing cytosolic pH and the regulation of intracellular pH
… H+ consumption are parts of a metabolic pH-stat mechanism which ameliorates the intracellular pH decline associated with anaerobiosis or other treatments. To test this hypothesis GABA production and efflux have been measured in isolated Asparagus sprengeri cells in response to three …
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Dilution acidosis : the effects of hyperosmolality on acid-base balance and ventilation
… by acute hyperosmolality was accompanied by an intracellular contraction alkalosis, implying the uncoupling of intra- and extracellular pH. The increase in extracellular [CF] and the pH changes suggested a primary role for CF/HCO3-exchange during this perturbation. However, both the …
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Absolute Concentration Determination of Phosphorus Metabolites in the Langendorff-Perfused Rabbit Heart by Phosphorus-31 Nuclear Magnetic Resonance (Quantification, Metabolism, Intact Tissue, Nmr)
The concentrations of mobile high energy phosphorus metabolites and intracellular pH of Langendorff-perfused rabbit heart have been determined under control and reduced flow conditions. Absolute concentration determination was accomplished by Lorentzian lineshape analysis after development of …
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Monitoring single heart cell biology using lab-on-a- chip technologies
… "active" assays, where the cell‘s metabolic and physiological function is itself controlled on-chip. The heart attack is considered the largest cause of mortality and morbidity in the western world. Dynamic information during metabolism from a single heart cell is difficult to obtain. There is a …
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Regulation Of Apoptotic Alkalinization Through Phosphorylation Of Sodium Hydrogen Exchanger Via P38 Mitogen Activated Protein Kinase
Regulation of intracellular pH is responsible for many cellular processes, such as metabolism, cell cycle progression, and apoptosis. Many chemotherapeutic agents work by inducing target cells to undergo apoptosis, a cell death process still poorly understood. Previous studies demonstrated that a …
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Improvement in viability of Lactobacillus Acidophilus in yogurt by stress adaptation
… organisms during storage is the decrease in intracellular pH caused by accumulation of organic acids in the yogurt. This leads to inactivation of essential metabolic pathways, deprivation of ATP to the cell, and eventually, cell death. A strategy to improve the viability of Lb. acidophilus in …
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Geochemistry and Microbiology of Extremely Alkaline (Ph > 12) Ground Water in the Calumet Slag -Fill Aquifer
… and with the need to maintain a circum-neutral intracellular pH (Horikoshi and Akiba, 1982). Here I describe a diverse microbial community that inhabits extremely alkaline (pH ≈ 12--13) groundwater from the Lake Calumet region Using microbial culturing, genetic sequencing, and microcosm …
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Non-Invasive Detection of Anaerobic Metabolism in Exercising Muscle Using Proton MRI
… that the T2 increase results from increased intracellular volume (VI), decreased intracellular pH (pHI), and/or changes in magnetization transfer (MT) or other longitudinal relaxation mechanisms. Isolated frog gastrocnemii were treated with iodoacetate (IAA; to inhibit lactate production), …
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Metabolic Changes in Astrocytes Following Traumatic Brain Injury
… ensure stoichiometric balance across the model. Physiologically relevant constraints from experimentally derived astrocyte metabolite uptake rates bounded upper and lower limits of astrocyte metabolism. Hypoxia and acidosis were simulated by restraining extracellular oxygen uptake rate and the …
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Characterizing The Regulation of Candida Albicans Hyphal Morphogenesis In The Context of Host Macrophages
… <em>C. albicans</em> to commensal niches. Macrophages are sentinel cells equipped with the ability to engulf and exterminate invading pathogens while simultaneously activating and recruiting other immune effectors. However, <em>C. albicans</em> is able to adapt to the hostile environment of the …
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Mutations in Connexin43 and Connexin40 Associated with Human Atrial Fibrillation; Physiology and Mechanism
… in HeLa cells using confocal microscopy. Electrophysiological studies were performed by a member of our lab to test the function of mutant gap junction channels. The Cx43 frameshift mutant (Cx43-932delC) caused intracellular retention of Cx43, resulting in impaired electrical coupling between …
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The role of respiration-dependent proton translocation in the acid tolerance of Gluconobacter oxydans
… cells were exposed to 0.1 M or 0.5 !vt NaCI at pH 3.20; subsequent alkalinization of the suspending solution suggested a NaCl-dependent flow of protons (H+) into the cells. Cells were then exposed to NaCI at pH 3.20 followed by the addition of glycerol to determine whether polyol oxidation …
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Engineering Hv1 Channel Mutations and Targeted Drug for Treatment of Hv1 Mutation Associated with Cancer
… channel Hv1 is crucial for maintaining cellular pH homeostasis by regulating proton movement across membranes, playing a vital role in acid extrusion and intracellular pH regulation. Dysregulation of Hv1 is associated with various diseases, including cancer, where it helps maintain an acidic …
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The development of motility in spermatozoa
… This study on rat spermatozoa shows that pH, cAMP and protein kinase C (PKC) all play an important role in the initiation of motility. pH has the most critical role and until the initial pH change in spermatozoa has occurred between the caput and caudal epididymal regions of the rat, …
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Elucidating mechanisms of toxicity and engineering Escherichia coli for tolerance of short chain fatty acids
… membrane leakage, membrane lipid analysis, hydrophobicity, carboxylic acid production, transcriptome analysis, intracellular pH, γ-amino butyric acid determination, proton motive force simulation, genome reverse engineering, and biocatalyst zeta potential. These methods allow greater insight into …
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The regulation of division of higher plant cells
… cells were found to rapidly become nitrate and phosphate limited in batch culture. The addition of these nutrients after each cell division resulted in several highly synchronous divisions (80-90%). In"these cultures 'the IAA levels remained at a relatively stable level (50 ng 108 cells). Thus, …
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Histidine Folding Switch In Effector Protein Avrpto Regulates Its Transport Through The Bacterial Type Iii Secretion System, As Determined By Nmr, Fluorescence, Circular Dichroism Spectroscopies And Translocation Assays
… histidine 87 that acts in the moderately acidic pH range expected in the biological environment of the bacterium. At neutral plant host cytosol of pH ~7.4 AvrPto is predominantly in the folded form. We disabled the His 87encoded pH switch by the H87Y mutation and observed a slight increase in …
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