Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"Intracellular delivery"”.
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Intracellular delivery of rabbit monoclonal antibody
… were discovered to be able to facilitate the delivery of small proteins into living cells. With the specific feature, researchers have successfully delivered some functional proteins into living cells. To fully explore and understand the functions and structures of intracellular proteins, more …
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Metal-organic frameworks as intracellular delivery vectors
… have emerged as interesting candidates for intracellular carrier-based delivery. These hybrid materials are constituted of metal clusters linked together by organic ligands. The possibility to tune their physical and chemical properties both in the bulk and at the surface allows for the …
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Engineering targeted proteins for intracellular delivery of biotherapeutics
… and mediate complex biological responses. The intracellular delivery of biotherapeutics is an unmet scientific challenge and medical need. A wide variety of different treatment modalities depend on not only on the ability to achieve intracellular delivery, but to do so in a targeted manner. An …
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Controlled Intracellular delivery of molecules using Nanoparticle-mediated Photoporation
Intracellular drug delivery has been of interest in recent years after the development of drugs targeting intracellular components. Delivery of exogenous material into cytosol has a variety of applications including cellular studies, therapies and diagnostics. Nanoparticle-mediated photoporation is …
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Reversibly neutralized perfringolysin O for intracellular delivery of macromolecules/
… this thesis, we describe the development of an intracellular delivery system based on a bacterial pore-forming protein, and demonstrate its efficacy in vitro using protein and siRNA payloads. Perfringolysin O (PFO) is a member of the cholesterol-dependent cytolysin (CDC) family of bacterial …
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Polymerizable Peptide Monomers for the Targeted and Intracellular Delivery of Cancer Therapeutics
… of peptides can be realized, a number of drug delivery barriers must be overcome. Namely, peptides have short circulation half-lives, are susceptible to degradation by extracellular proteases, and are unable to cross cell membranes and access intracellular targets. An antibody-targeted, …
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Cell squeezing : a vector-free microfluidic platform for intracellular delivery of macromolecules
Intracellular delivery of material is a long-standing challenge for both therapeutic and research applications. Existing technologies rely on a variety of mechanisms to facilitate delivery. Vector-based methods, such as polymeric nanoparticles and liposomes, form complexes with the target material …
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Intracellular delivery and voltage sensitivity of nanomaterials for the optical imaging of neuronal activity
… nanomaterials (VSM) in live neurons is their delivery into the plasma membrane bilayer. Currently, the delivery has been demonstrated on QDs via their spontaneous insertion directly into the plasma membrane bilayer, or indirectly into the bilayer of liposomes that later fuse with the plasma …
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Development of Shell-Crosslinked Knedel-like Nanoparticles as Intracellular Delivery Vehicles and Gene Regulation Agents
This dissertation focuses on the development of polymer-based nanomaterials, termed shell-crosslinked knedel-like: SCK) nanoparticles, as vehicles to carry specific guest molecules or guest structures into the cell. Detailed synthetic procedures for and characterization of well-defined block …
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A novel intracellular protein delivery system - Magnesium phosphate nanoparticles with cationic lipid coating for catalase intracellular delivery
… those caused by alternations in the functions of intracellular proteins. However, clinical use of protein by intracellular delivery has been hampered by the instability due to proteins' physicochemical properties, and some barriers in the delivery pathway. This study was to prepare and test a …
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Investigation of a fusogenic pH-sensitive liposomal system for tumour-targeted intracellular delivery of dinitrobenzamide mustard prodrugs
… cellular uptake, limits their potency. In drug delivery, nano-sized PEGylated pH-sensitive liposomes (pPSL) have been investigated as an effective drug carrier (and solubiliser) to confer improved tumour targeting. pPSL containing phosphatidylethanolamine are fusogenic and can achieve …
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Studies on the efficient intracellular delivery vehicle using variable region domain of a cell-penetrating anti-DNA monoclonal antibody
… the applicability of the domain as a cellular delivery vehicle. To evaluate the cell-penetrating property of 2C10 VH domain in various mammalian cell lines, the flow cytometric and confocal microscopic analysis and cell proliferation assay were used. The VH domain penetrated into all cell lines …
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Combinatorial lipid-like materials for intracellular delivery of small RNAs that activate innate antiviral immune responses and adjuvant vaccines
… application will require novel systems for intracellular delivery. A library of structurally-related cationic lipid-like materials, termed "lipidoids", was developed to facilitate uptake of small RNAs, and the role of drug delivery in controlling RNA function was investigated. In an …
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Convective intracellular macromolecule delivery for cell engineering applications
Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering, cell labeling, and other biomedical applications. Current standard methods of intracellular delivery, such as viral transduction and electroporation, do not meet the growing needs in the cell …
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Development of polymer and lipid materials for enhanced delivery of nucleic acids and proteins
… of synthetic vectors enabling efficient intracellular delivery of macromolecular therapeutics such as nucleic acids and proteins could potentially catalyze the clinical translation of many gene and protein-based therapies. However, progress has been hindered by a lack of safe and …
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The Engineering and Development of pH-Responsive Biopolymers for Drug Delivery Applications
… with phenylalanine was able to enhance the intracellular delivery efficiency of the polymer. However, the exact structure and mechanism of action remains mostly unknown. In this study, a variety of modifiers was used to synthesise new PLP derivatives in order to elucidate their effect on the …
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Engineering bacteriophage nanocarriers for targeted delivery of protein reagents to prostate cancer cells
… of most proteins greatly inhibits their intracellular delivery to diseased cells, limiting targets to those displayed on the cell surface. In response to this challenge, we have developed a bacteriophage nanocarrier to deliver large payloads of proteinaceous cargo to the interior of …
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Development of chemical probes for intracellular nucleotide delivery, profiling of the metabolic fate(s) of nucleoside monoester phosphoramidates, and a nucleotide mimetic inhibitor of eIF4E
… the development of phosphate prodrugs for intracellular delivery of monophosphates. Such efforts led to the successful application of the aryloxy amino acid phosphoramidate (ProTide) strategy for development of sofosbuvir. Although widely successful, several drawbacks of the ProTide …
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