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Showing 1 to 20 of 67 for “"Internal transcribed spacer"”.

  1. Phylogenetic Analysis of Iliamna (Malvaceae) Using the Internal Transcribed Spacer Region

    Bodo Slotta, Tracey A.Virginia Tech2000

    … a phylogeny for Iliamna using sequences from the internal transcribed spacer region (ITS) of the nuclear ribosomal RNA genes in order to determine its biogeographical and evolutionary history. Cladistic analysis was performed and the resulting phylogeny is presented. The ITS data provide new …

    vt Repository record

  2. Using Molecular Diagnostics Based On Internal Transcribed Spacer 2 Sequence To Study Geographical Distribution of Holarctic Malaria Mosquitoes

    Hodge, James MichaelVirginia Tech2020

    … For identification, sequencing of the Internal Transcribed Spacer 2 region of the ribosomal DNA is analyzed. Although African Anophelines are very well studied species, there has been no recent significant research for Holarctic Anophelines. In particular, in North America and Eurasia, …

    vt Repository record

  3. Towards a molecular approach for the identification of fungal taxa that contain psilocybin

    Lee, James Chun-IUniversity of Strathclyde2001

    … subunit ribosomal DNA (nuc-ssu-rDNA) and the internal transcribed spacer 1 (ITS-1) DNA regions. The sequences of 877 bp DNA fragment of nuc-ssu-rDNA exhibited genus specific DNA sequences. The size (307-344 bp) and sequences variation of ITS-1 showed not only genus-specific but also …

    strathclyde Repository record

  4. Phylogeographic patterns in three South African forest mosses

    Mwafongo, ElizabethDepartment of Biological Sciences2003

    … of forest patches, two molecular markers, internal transcribed spacer (ITS) nuclear rDNA and trnL-F cpDNA were employed.

    cape-town Repository record

  5. Evaluating oxalate-degrading Lactobacillus spp. for their ability to be used as probiotics in the treatment of kidney stone disease

    Kabanda, Siti MDepartment of Molecular and Cell Biology2010

    … of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer (ITS) region. The strains were identified as follows; Lactobacillus gasseri 7(3), L. gasseri 17(4), Lactobacillus reuteri 17(7) and L. reuteri 16(9). Their probiotic characteristics were also evaluated.

    cape-town Repository record

  6. Cyperaceae tribe Cyperae : phylogenetic relationships and evolutionary patterns of diagnostic characters

    Dludlu, Meshack NDepartment of Biological Sciences2007

    … of plastid DNA (rbcL, trnL-F intergenic spacer, and rps16), nuclear ribosomal DNA (internal transcribed spacer ITS) sequences and morphological data. From both analyses, tribe Cypereae were resolved into a very strongly supported clade (Posterior probability =98%; Bootstrap= 100%) …

    cape-town Repository record

  7. A multilocus phylogeny of Hericium fungi in Canada and their production of Erinacine A

    Koga, JulienThe University of Western Ontario2024

    … phylogenies of the genus Hericium based on the internal transcribed spacer (ITS) region struggle to support monophyly at the species level, hindering biochemical research efforts. Using concatenated sequence data from ITS, the large ribosomal subunit (LSU), translation elongation factor 1-alpha …

    uwo Repository record

  8. Isolating and Identifying Fungal Endophytes from Roots of Rare Orchid Cypripedium arietinum in Grand Sable Dunes, Michigan

    Farkas-Lasich, Kari2019

    … conserved 5.8s rDNA and the highly variable internal transcribed spacer (ITS) regions on either side of it. DNA was sequenced and sequences were identified using NCBI BLAST. Of the 36 DNA samples 27 were positively identified. <em>Phialocephala fortinii</em> and <em>Leptodontidium …

    nmu Repository record

  9. Diversity of the marine cyanobacterium Trichodesmium : characterization of the Woods Hole culture collection and quantification of field populations

    Hynes, Annette MichelleMassachusetts Institute of Technology2009

    … two clades based on sequences from 16S rDNA, the internal transcribed spacer (ITS), and the heterocyst differentiation gene hetR. Clade I contained Trichodesmium erythraeum and Trichodesmium contortum, and clade II contained Trichodesmium thiebautii, Trichodesmium tenue, Trichodesmium …

    mit Repository record

  10. Belowground Fungal Community Change Associated with Ecosystem Development

    Pineda Tuiran, Rosana P.Virginia Tech2017

    … by pyrosequencing the soil DNA, targeting the internal transcribed spacer region. Chapter 3 contains a coauthored published paper that describes plant invasion of fields in Virginia to determine how they impact soil bacterial and fungal communities. The bacterial and fungal communities that …

    vt Repository record

  11. Isolation and molecular characterization of Non-Tuberculous Mycobacteria of Public Health significance from Humans and water in Namwala Districts of Zambia

    Monde, NgulaThe University of Zambia2016

    … were then characterized using the 16S-23S internal transcribed spacer region Sequencing. One hundred and fifty three (153) individuals with suspected TB were sampled and 7(4.6%) were found to have NTM with M. arupense (3, 2%) being the most common organism. Out of the 149 water samples …

    zimbabwe Repository record

  12. Isolation and molecular characterization of Non-Tuberculous Mycobacteria of Public Health significance from Humans and water in Namwala Districts of Zambia

    Monde, NgulaThe University of Zambia2016

    … were then characterized using the 16S-23S internal transcribed spacer region Sequencing. One hundred and fifty three (153) individuals with suspected TB were sampled and 7(4.6%) were found to have NTM with M. arupense (3, 2%) being the most common organism. Out of the 149 water samples …

    zambia Repository record

  13. Mentha (Lamiaceae) Phylogenetic Analysis Using Chloroplast TRNL-TRNF and Nuclear Ribosomal DNA ITS Sequences

    Bunsawatt, Jiranan

    … the chloroplast trnL-trnF and nuclear ribosomal internal transcribed spacer (ITS) regions. Based on trnL-trnF data, Mentha appears monophyletic. However, ITS data place the Mentha species into two distinct clades that include 12 other Mentheae genera. None of the sections with more than one …

    wku-diss Repository record

  14. Resolving a Cryptic Species Complex: Application and Utility of Mitochondrial 16S and Nuclear Its1 Molecular Markers in Pyrgulopsis Micrococcus

    Kepes, Steven

    … DNA region (16S) and the complete first nuclear internal transcribed spacer region (ITS1) were performed for multiple individuals from each of thirteen populations of P. micrococcus, ten other Pyrgulopsis species, and an outgroup species from the genus Nymphophilus. Neighbor-joining and maximum …

    mo-state Repository record

  15. A molecular characterization of the fungal endophytes within the needles of ponderosa pine (Pinus ponderosa)

    Gray, Amy E.

    … was isolated directly from needles of two ages. Internal transcribed spacer regions of the ribosomal RNA gene form fungi were amplified by PCR, cloned, and sequenced. Many of the sequences described have high homology matches in GenBank with known fungi, though most have not previously been …

    eastern-wash Repository record

  16. Nymphaea odorata (Water-lily, Nymphaeaceae): Analyses of molecular and morphological studies

    Woods, Kristi YvonneVirginia Tech2003

    … odorata. Molecular analyses of the nuclear internal transcribed spacer (ITS) region, the chloroplast trnL-F region, and inter-simple sequence repeat (ISSR) markers determined the variation present between and within two species of Nymphaea. The ITS region resulted in a phylogeny depicting …

    vt Repository record

  17. Assessment of species boundaries of the Moringa Ovalifolia (Dinter & A. Berger) in Namibia using nuclear its DNA sequence data

    Hausiku, Martha KasikuUniversity of Namibia2019

    … ovalifolia in Namibia by using the nuclear Internal Transcribed Spacer (ITS) sequenced data analysis. A total of seventeen (17) edited sequences of M. ovalifolia together with thirteen (13) other sequences of Moringa retrieved from NCBI were used to construct phylogenetic trees in Molecular …

    namibia Repository record

  18. A Systematic Revision of Bakeridesia Hochr. (Malvaceae)

    Donnell, Aliya A.Ohio University2012

    … relationships. First, phylogenetic analyses of Internal Transcribed Spacer (ITS) sequences provided strong evidence that Bakeridesia is polyphyletic if circumscribed to include the Brazilian subgenus Dipteron. Consequently, the clade comprising subg. Dipteron and several species previously …

    ohiolink Repository record

  19. Molecular Population Genetics of the Atlantic Sand Fiddler Crab, Uca Pugilator, Along the Atlantic Coast

    Weese, David A.2014

    … of the mitochondrial 16S rRNA and the nuclear internal transcribed spacer 1 (ITS1) genes of these individuals. The ITS1 marker did not prove to be informative when screened by SSCP for this study. The 16S marker revealed a moderate amount of population structure (FST = 0.292) between …

    gsu Repository record

  20. Diversity of fungi associated with dieback of Ziziphus Mucronata in Limpopo Province, South Africa

    Thaphathi, Polly2020

    … data from multiple genome regions including the internal transcribed spacer (ITS), beta-tubulin (BT) and the translation elongation factor (TEF) genomic regions and phylogenetic analyses. Fungi identified in this study were from families Botryosphaeriaceae, Diaporthaceae, Cytosporaceae …

    venda Repository record

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