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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Interference microscopy"”.
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Spatial light interference microscopy and applications
Phase contrast microscopy has revolutionized cell biology by rendering detailed images from within live cells without using exogenous contrast agents. However, the information about the optical thickness (or phase) is qualitatively mixed in the phase contrast intensity map. Quantifying optical …
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Quantitative anisotropy imaging with polarized interference microscopy
… developed, such as fluorescence polarization microscopy(FPM), liquid crystal based polarization microscopy (LC Polscope), polarization state optical coherence tomography (ps OCT), and polarization Raman spectroscopy. Quantitative phase microscopy (QPM) is another important modality of optical …
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Gradient light interference microscopy for imaging strongly scattering samples
… systems has raised new challenges for light microscopy. The fundamental difficulty is the tendency for the optical field to scramble when interacting with turbid media, leading to contrast images. In this work, we outline the development of an instrument that uses broadband optical fields in …
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LIVE CELL IMAGING BY INTEGRATING FLUORESCENCE AND MOTION- ENHANCED DIFFERENTIAL INTERFERENCE MICROSCOPY
Optical microscopy's resolution is limited by the wavelength of illuminating light and can be improved by selecting shorter wavelengths. The contrast of optical microscopy, which along with resolution, ultimately determines image quality and is enhanced in techniques such as differential …
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Spatial light interference microscopy (slim) measurements of hypothermia in an experimental cellular model of traumatic brain injury
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-12-04 without embargo terms
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Computational phase imaging for biomedical applications
… new QPI setup, named transmission Spatial Light Interference Microscopy (tSLIM), that uses the twisted nematic liquid-crystal (TNLC) modulators. Compared to the established SLIM technique, tSLIM is much less expensive to build than its predecessor (SLIM) while maintaining significant performance. …
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Engineered hydrogels for tracking cell signaling from the outside-in
… polyacrylamide model system with spatial light interference microscopy (SLIM) in order to design a novel patterning approach to simultaneously measure cell traction force along with cell growth and migration. This allows us to study the interaction between cells and ECM to understand the …
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miR-125b toggles dynamics and structure of dendritic filopodia in developing hippocampal neurons
… filopodial morphology. Using Spatial Light Interference Microscopy (SLIM), we show that miR-125b function contributes to maintaining the dynamicity of filopodia. We propose that miR-125b is critical in maintaining the filopodial phenotype early in dendrite development, thus contributing to …
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Spectral domain interferometry: A high-sensitivity, high-speed approach to quantitative phase imaging
… microscopes. Therefore, the phase-based optical microscopy techniques play important roles in the development of the modern biomedical science. Furthermore, the ability to achieve quantitative phase measurement of the tiny structures of biomedical specimens is of great importance for many …
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Novel methods for recording and reconstructing images in digital holographic microscopy
… unstained biological cells using brightfield microscopy has resulted in the development of several specialized imaging techniques that can enhance the contrast of subcellular features without the need for labeling. Examples include phase contrast, differential interference microscopy, dark …
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Quantitative phase imaging: advances to 3D imaging and applications to neuroscience
… and advances made on them. First, spatial light interference microscopy (SLIM) is introduced as a QPI method extensively used in this thesis. Using this setup, an application of QPI in neuroscience is demonstrated by studying the emergent formation of a neuronal network. Second, an expansion of …
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Neuronal filopodia borne along tips and shafts of dendrites comprise two distinct populations as evidenced by differences in structure and dynamics
… Semaphorin3A (Sema3A). Using confocal microscopy to visualize filopodial membrane and actin we found that shaft filopodia are shorter than tip filopodia, and show a less dense presentation along the dendrite. We then employed the quantitative phase imaging technology of Spatial Light …
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Adsorption in Confined Aqueous Films
… Debye-length. The separation was measured using interference microscopy and the adsorption of fluorescein was measured using fluorescence microscopy. The Debye-length was altered by variation of the background salt (NaCl) concentration in dilute (<1 M) solution. The surface excess of adsorption …
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Quantitative light imaging of intracelluar transport
… using a new application of spatial light interference microscopy (SLIM) (Chapter 1). We used this quantitative phase imaging method to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach, Dispersion-relation Phase …
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Redox manipulation alters hippocampal neuron response to semaphorin 3A
… effects of the treatments using spatial light interference microscopy (SLIM) in real time. The effects of reducing conditions on dendritic growth was consistent with the initial hypothesis but the effects of oxidizing conditions revealed a more complex relationship between Sema3A and redox …
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Label-free breast histopathology using quantitative phase imaging
… phase imaging (QPI). QPI is a label-free microscopy technique where image contrast is generated by measuring the optical path-length difference (OPD) across the specimen. OPD refers to the product of the refractive index and thickness at a point in the field of view. Since this measurement …
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Using CRISPR/Cas9 to modify the genome of cattle
… taken of the sperm using the Spatial Light Interference Microscopy (SLIM) confirmed the presence of liposomes on the sperm head and tail. SMGT was then used with IVF to deliver two plasmids containing GFP encoding region under the control of CMV promoter; pIRES2-EGFP and pSpCas9(BB)-2A-GFP …
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High throughput platform for multiscale quantitative phase imaging
Quantitative phase imaging (QPI) yields the spatial phase map of the object’s scattering potential. QPI has enabled unprecedented label-free studies in biomedicine, ranging from cell dynamics and growth to cancer diagnosis and prognosis. The field is currently transitioning from technology-driven …
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Prostate tissue diagnosis using quantitative phase imaging techniques
Restriction data tranferred 2014-07-01T11:20:34-05:00 Original Data Group with Access UIUC Users [automated] Release Date: 2015-08-22 11:49:27 UTC Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system
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