Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Integrin Activation"”.
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The membrane lipid composition regulates talin mediated integrin activation
Integrins are a family of transmembrane receptors that mediate cell-cell and cell-extra cellular matrix (ECM) interaction and signaling. Inside-out activation of integrin receptors often requires the binding of the cytoplasmic domain of the subunits by talin. This interaction leads to separation of …
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Role of Talin1 Phosphorylation In Beta1 Integrin Activation and Prostate Cancer Metastasis
… regulate focal adhesion signaling by conjugating integrins to the cytoskeleton. Talins directly bind and activate integrins but the mechanism by which this occurs is unknown. As integrin activation and overexpression of talins promote prostate cancer metastasis, understanding the mechanism by …
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Chemomechanical regulation of integrin activation and cellular processes in acidic extracellular pH
… to acidic pHe are largely unknown. Transmembrane integrin receptors form a physical linkage between cells and the extracellular matrix, and are thus capable of modulating cell adhesion and migration in response to extracellular conditions. In this thesis, computational and experimental approaches …
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Development of a Model System for Culturing Neonatal Rat Ventricular Myocytes in vitro to Monitor Integrin Activation in Response to Altered Contractile Force
… by the contractile apparatus and sensed by integrins is central to understanding the pathogenesis of HCM. Integrins are likely the mechanotransducers in cardiomyocytes, converting mechanical stress into chemical signals that stimulate cardiac hypertrophy. Outside-in integrin signaling has …
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Signaling of integrin lower leg and transmembrane domains
Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it has been shown that TM and/or cytoplasmic α and β domains associate in the resting state and separation of these domains is required for integrin …
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Inhibiting platelet scramblase activity
… platelets. Pro-aggregatory platelets have active integrins and aggregate together via fibrinogen to form the haemostatic plug which prevents bleeding. Pro-coagulant platelets expose phosphatidylserine (PS) which acts as a surface for binding of the tenase (FIXa/VIIIa) and prothrombinase (FXa/Va) …
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Molecular Mechanisms Of Pressure-Stimulated Cancer Cell Signaling
… by a mechanism that is dependent upon beta-1-integrin activation, an intact cytoskeleton, and FAK and Src activation. By a different mechanism, increased extracellular pressure modulates cancer cell proliferation in a manner that is regulated by protein kinase C, but not Src or an intact …
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Understanding the adhesome network in primary and metastatic cutaneous squamous cell carcinoma
… shuttling of Mena is regulated by integrin activation and focal adhesion kinase (FAK). Moreover, our preliminary results suggest that Mena may act as a molecular clutch for the mechanosensing function of Nesprin-2 with actin. The putative molecular clutch activity of Mena regulates …
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Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
… II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation induced by spatially activated small GTPase Rho and its downstream kinase p160-ROCK, as previously reported, leads to contraction of actin-myosin II complexes at the …
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The Effect of Manipulation of the Phosphoinositide 3-kinase Pathway on Axon Regeneration in vivo
… The PI3K pathway is activated by growth factors, integrins and cytokine receptors. Integrins have also been shown to promote axon regeneration via the focal adhesion kinase pathway. This thesis aimed to determine the effects on axon regeneration of manipulating the PI3K pathway in various ways. A …
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Nephrotic Syndrome and Glomerular Basement Membrane: Genetic Defect of the Laminin α5 Chain
… proteomics analysis and investigation of integrin activation, revealed a possible impact of the causative mutation on protein folding. Data suggest that changes in protein structure lead to a reduced secretion, integrin β1 activation, and agrin expression ultimately resulting in possible …
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Mesothelial and Mural Cell Contribution to Vascular Development through PDGF Signaling
… tyrosine kinase with high homology in signal activation to PDGFR alpha. Downstream signaling pathway activation includes PI3 kinase, Src, RasGAP, Grb2, Shp-2, and PLC?gamma for the regulation of cellular functions.The focus of this research was to determine the temporal and functional …
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Combination of Dasatinib and Dexamethasone targets the myeloma plasma cell through its interaction with the bone marrow microenvironment
… of plasma cells on Fibronectin (FN) despite integrin activation. This effect correlated with down regulation of Src and Abl activity. Both Dasatinib and Dexamethasone inhibited adhesion of PC on stromal cells and osteoclasts. Dasatinib disrupted the organisation of actin cytoskeleton in …
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Fluorescence resonance energy transfer-based subcellular visualization of FAK/Src activation by chemical and mechanical environment
… In response to chemical stimulations, the activation of Src population at lipid rafts was slower and weaker compared to that outside lipid rafts. In contrast, FAK biosensors targeted to different membrane microdomains revealed that FAK activation mainly occurs at lipid rafts in response to …
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Molecular mechanisms of signal transduction in neutrophil chemotaxis
… kinase Lyn that is essential for localized integrin activation, leading edge attachment, and persistent migration during neutrophil chemotaxis. This pathway depends upon Gi protein-mediated activation and leading edge recruitment of Lyn. We documented the small GTPase Rap1 as a major …
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VE-cadherin mechanotransduction signaling and its regulation by extracellular matrix
… signaling cascade that involves VEGFR2, PI3K, Integrins, FAK and RhoA GTPase. The signaling also leads to vinculin and actin recruitment downstream of integrin activation, which is a potential mechanism to protect the cell-cell junctions against disruption. Chapter 3 investigates how …
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Identification of novel chemokine and chemokine-like mechanisms in leukocyte adhesion and atherosclerotic lesion formation
… for MIF is CD74, which can mediate sustained activation of ERK/MAPK. Yet, no receptor had been discovered that would be able to confer MIF’s chemokine-like functions in cells devoid of CD74, as neutrophils and fibroblast, which are also affected by MIF. This thesis introduces the two chemokine …
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Regulation of Protein Kinases (Syk and PKC zeta) in platelets
… receptor systems leads to alpha IIb beta 3 activation, thromboxane generation, ADP release, etc., culminating in platelet clot or thrombus formation. The protein kinase family of enzymes mediates a significant number of these intracellular signaling events that culminate in platelet …
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MOLECULAR PHYSIOLOGY OF THROMBOXANE A2 GENERATION IN PLATELETS
… dense granules and is released upon platelet activation. ADP activates platelets through two purinergic receptors namely P2Y1 and P2Y12 these receptors couple to Gq and Gi class of G-proteins, respectively. P2Y1 causes calcium mobilization through activation of PLC-β. P2Y12 inhibits adenylyl …