Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 64 for “"Integrative Biomedical Sciences"”.
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Identification of a novel HIV-1C protease from a microbial source
HIV-1 subtype C is currently the most prevalent in the epidemiology of HIV/AIDS cases in subSaharan Africa. Most clinical protease inhibitors (PIs) were designed against subtype B and are reported to have reduced activity against subtype C proteases. Our initial hypothesis was to create an …
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Molecular and Kinetic Characteristics of wild type and mutant Porphobilinogen deaminase
The purpose of this dissertation was to provide an overview of acute intermittent porphyria, focussing on the structure and function of the enzyme, porphobilinogen deaminase (PBGD), as well as experimentally demonstrating the use of kinetic, structural and thermodynamic approaches to shed light on …
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Establishment of recombinant antibody technologies allowing for the generation of SNAP-tag fusion proteins
Triple negative breast cancer (TNBC's) is a highly aggressive and invasive subtype of breast cancer, typically characterised by the lack of estrogen receptor (ER), progesterone receptor (PR) and Human epidermal growth factor receptor 2 (HER2) with an inexplicable partiality towards African women. …
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Testing for host adaptive evolution using the maize streak virus model
Maize streak virus (MSV; Genus: Mastrevirus; Family: Geminiviridae) causes maize streak disease (MSD); a major biotic threat to maize farming especially in sub-Saharan Africa, and it neighbouring Indian and Atlantic Ocean Islands, where its insect vectors in the genus Cicadulina thrive. Of the …
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Integration of multi-omic data and neuroimaging characteristics in studying brain related diseases
Approaches to the identification of genetic variants associated with complex brain diseases have evolved in recent decades. This evolution was supported by advancements in medical imaging and genotyping technologies that result in rich data production in the field of imaging genetics and …
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Investigating local ancestry inference models in mixed ancestry individual genomes
Owing to historical events including the slave trade, agricultural interests, colonialism, and political and/or economical instability, most modern humans are a mosaic of segments originating from different populations. They result from the interbreeding of two or more previously isolated …
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Characterisation of Kaposi's sarcoma-associated herpesvirus (KSHV)-driven pathology and disease outcome in HIV infected South African patients
Kaposi's sarcoma-associated herpesvirus (KSHV), a gamma-herpesvirus with a particularly high seroprevalence in Sub-Saharan Africa (SSA), is the etiological agent of the endothelial tumour Kaposi's sarcoma (KS), the most common acquired immunodeficiency syndrome (AIDS)-related malignancy worldwide …
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Transcription analysis of virulent strains of Mycobacterium tuberculosis
Background: Despite the development of new drugs and success of social programs, tuberculosis remains a leading cause of mortality. This burden falls disproportionately on developing countries where the high burden of HIV has a potentiating e↵ect, but may soon return to areas where it was …
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Molecular and Kinetic Characterisation of wild type and mutant Porphobilinogen Deaminase
The purpose of this dissertation was to provide an overview of acute intermittent porphyria, focussing on the structure and function of the enzyme, porphobilinogen deaminase (PBGD), as well as experimentally demonstrating the use of kinetic, structural and thermodynamic approaches to shed light on …
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Proteomic insights into the modulation of foetal neurogenesis by the anti-retroviral efavirenz
Background: South African guidelines recommend that HIV-positive pregnant women immediately initiate antiretroviral therapy (efavirenz, emtricitabine, and tenofovir), regardless of trimester. Efavirenz causes central nervous system neuropathy and has been linked to birth defects such as …
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The use of evaluation in the design and development of interactive medical record systems
An explorative study was done to develop an evaluation methodology. This method can be applied during the development of interactive medical record systems in order to provide information which can be used to improve user interaction with the system. Th e evaluation methodology consists of a number …
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Analysis of driver gene mutations in oesophageal squamous cell carcinoma
Oesophageal cancer (OC) is the eleventh most diagnosed cancer and the seventh most common cause of cancer-related deaths worldwide. The two main subtypes are oesophageal adenocarcinoma (OAC) and oesophageal squamous cell carcinoma (OSCC). OAC is more common in North America and Europe, while OSCC …
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An electrocardiograph tutor using expert system technology
Computer systems for the interpretation of diagnostic ECGs are widely used, but currently provide no explanatory or teaching functions of value to the less experienced practitioner. The relevant literature is reviewed, and specifications are provided for an ECG analysis system which will function …
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High-Throughput Determination of Mycobacterium smegmatis Protein Complex Structures
Tuberculosis (TB) is an endemic health-crisis, particularly in sub-Saharan Africa. The rise of multiand extensively-drug resistant Mycobacterium tuberculosis (Mtb), the causative agent of TB, has led to further developments in understanding the physiology of Mtb during infection, as well as …
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Investigating nuclear transport proteins as secreted cancer biomarkers
Previous studies in our laboratory using microarray gene expression analysis identified members of the nuclear transport protein family as significantly upregulated in cervical cancer biopsies compared to normal cervical epithelial tissues. These results were validated at both mRNA and protein …
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An African Genome Variation Database and its applications in human diversity and health
African genomes exhibit the highest levels of sequence and haplotype diversity of all extant human populations. A combination of historical as well as geographical factors have contributed toward the high level of genetic diversity in Ancestral populations in Africa. Additionally, a series of …
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Characterisation of dysregulated proteins in macrophages infected with Mycobacterium smegmatis focusing on matrix metalloproteases and their effectors
Cavitation is a key facilitator in transmission of Mycobacterium tuberculosis (M. tb). Upregulation of matrix metalloproteases (MMPs) has been documented in patients with tuberculosis (TB), while their tissue inhibitor (TIMPs) levels remained the same. Animals which can develop cavities have …
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Cell-free DNA and tumor exosome cargo as diagnostic and prognostic marker for prostate cancer
According to the Global Cancer Statistics 2020, prostate cancer (PCa) is the second most commonly diagnosed male cancer and second leading cause of cancer death among men globally. Prostate cancer is known to be more aggressive among men of African origin with reasons not fully known. Previous …
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To compare the expression, processing, incorporation and function of pseudoviruses and infectious molecular clones using different cell types and HIV backbones
Understanding HIV transmission mechanisms is essential for the design and development of an efficacious, broadly acting vaccine that targets features common to transmitted viruses. However, there is a lack of consensus amongst current HIV studies characterising transmitted founders (TFs). When …
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Photoimmunotheranostic targeting of CSPG4-positive melanoma cells using SNAP-tag technology
Melanoma is one of the most aggressive and inherently resistant cancers and the most dangerous skin cancer. While it accounts for fewer than 5% of skin cancer cases, 80% of skin cancer related deaths are attributed to melanoma. While resection remains the gold standard for melanoma treatment, …
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