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Showing 1 to 20 of 52 for “"Integral membrane protein"”.
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Defining Topologically Oriented Transmembrane Helix Contacts in Integral Membrane Protein EmrE by a Modified Two-Hybrid Approach
Study of integral membrane proteins is comparatively difficult in comparison to soluble proteins, requiring more specialized techniques. Here is presented an application of the Bacterial Adenylate Cyclase Two-Hybrid (BACTH) method to study oligomerization in membrane proteins applied to model …
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The SAC1p, an integral membrane protein involved in secretory pathway function and actin function in Saccharomyces cerevisiae
… strictly analogous to that of mammalian cells. Proteins destined for secretion are transported in a vectorial fashion from the endoplasmic reticulum to the Golgi apparatus to the cell surface. Superimposed on the normal flow of secretory traffic in yeast is a level of spatial organization. …
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In vitro studies on the SecA involvement in the assembly of an integral membrane protein with large hydrophilic domain
The transport of secretory proteins across the inner membrane of E. coli as well as the integration of inner membrane proteins takes place via the SecYEG-translocon. The targeting pathways of secretory and membrane proteins to the SecYEG translocon however, are different. Integral membrane proteins …
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Mutational studies of the cytochrome bc(1) complex of Rhodobacter sphaeroides
The cytochrome $bc\sb1$ complex is a multisubunit integral membrane protein. This enzyme is a central component in electron transport where it oxidizes quinol while forming a transmembrane proton gradient. The cytochrome b subunit is the locus of two distinct quinol binding sites as well as the …
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Lateral organization and dynamics of phospholipids in membrane
… or phosphatidic acid. However, another integral membrane protein, D-$\beta$-hydroxybutyrate dehydrogenase (BDH), reorganized the phospholipids in the bilayer into a nonrandom distribution. Although the enzyme has a specific requirement for phosphatidylcholine for activity, the extent of …
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Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
… the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of membrane interaction such that P450 2C2 is no longer an integral membrane protein. Moreover, the results show that the linker domain is …
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Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2
… economic consequence. The PRRSV non-structural protein 2 (nsp2) is a large multifunctional protein encoded by the most genetically diverse region of the genome - the selective pressure potentiating mutation within this region is unknown. Here we report the identification of a unique function of …
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Exp1p is a cargo adaptor for Sec24p mediated export the plasma membrane H+̳ ATPase from the ER in S. cerevisiae
… the folding, modification and delivery of plasma membrane and secreted proteins. The secretory pathway consists of an ordered series of organelles including the endoplasmic reticulum (ER), Golgi, endosome and vacuole. In the ER, properly folded proteins are separated from misfolded and ER resident …
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PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
… glutamate transporter 1 (VGLUT1) is an important membrane protein located in glutamatergic synaptic vesicles. It is responsible for the storage and release of the excitatory neurotransmitter glutamate. VGLUT1 is a highly hydrophobic integral membrane protein with a molecular weight around 61 kD. …
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PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
… glutamate transporter 1 (VGLUT1) is an important membrane protein located in glutamatergic synaptic vesicles. It is responsible for the storage and release of the excitatory neurotransmitter glutamate. VGLUT1 is a highly hydrophobic integral membrane protein with a molecular weight around 61 kD. …
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Molecular dynamics studies of membranes and membrane proteins
Properties of phospholipid membrane bilayers and of proteins associating and functioning in the membrane bilayer or at the membrane-water interface are studied by combining molecular dynamics simulations, free energy perturbation theory and continuum electrostatics calculations. Three systems were …
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NMR structural and functional studies of the mithochondrial outer membrane protein VDAC by Thomas J. Malia.
… and for tissue maintenance. Bcl-2 family proteins are central regulators of apoptosis and many of their primary roles are carried out in the outer mitochondrial membrane. Anti-apoptotic Bcl-xL has been found previously to interact with VDAC, an outer mitochondrial membrane protein …
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Solid-state NMR investigations of the bacteriorhodopsin photocycle
Bacteriorhodopsin (bR) is a heptahelical integral membrane protein with a retinylidene prosthetic group from H. salinarum. The protein creates a proton gradient across the cell membrane by harvesting light energy. Proton transfer events can be segregated into transfers between the Schiff base of …
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Structural and Functional Insights into Ghrelin Acylation by Ghrelin O-Acyltransferase
… acts as the endogenous ligand for the GHSR1a G-protein-coupled receptor. This 28 amino acid peptide stimulates appetite and has been associated with the cardiovascular, gastrointestinal, neuroendocrine, and immune systems. Ghrelin requires a unique posttranslational modification to be biological …
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Enzymology and inhibition of ghrelin acylation by ghrelin O-acyltransferase
… (GOAT), a topologically complex integral membrane protein. GOAT is an intriguing target for biochemical studies, as no other enzyme is known to catalyze octanoylation of a serine hydroxyl group. It is also an attractive target for diseases related to ghrelin signaling, such as …
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The identification and characterization of novel persistence genes in chlamydia trachomatis
… mapped had mutations in CTL0225, a putative integral membrane protein, and CTL0694, a putative oxidoreductase. Neither of these genes plays a known role in tryptophan synthesis. However, amino acid (AA) competitive inhibition assays suggest that CTL0225 may be involved in the transport of …
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The AA3 Type Cytochrome C Oxidase From Rhodobacter Sphaeroides: Insights Into the Proton Pumping Mechanism From the N139D Mutation in the D-Channel
Cytochrome c oxidase, an integral membrane protein, is the final enzyme of the electron transport chain. The two sides of the membrane are denoted as the N side (the cytoplasmic side in bacteria and the matrix side of mitochondria in eukaryotes) and the P side (the periplasmic side in bacteria and …
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Formation of membrane domains induced by the G and M proteins of vesicular stomatitis virus
… specialized regions or domains on the plasma membrane of infected cells. These domains are composed mainly of viral proteins and they are enriched in certain lipid species. The properties of the two envelope-associated proteins of VSV, the glycoprotein (G) and the matrix protein (M), were …
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Magic angle spinning NMR applied to membrane protein 2D crystals : the structure and function of VDAC
Membrane proteins mediate critical functions in biological systems and are important drug targets for a number of diseases. Determining the three-dimensional structure and function of membrane proteins under physologically relevant conditions is a long sought-after goal in structural biology. In …
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