Global ETD Search
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Showing 1 to 9 of 9 for “"IntDOT"”.
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Characterization of IntDOT, the CTnDOT Integrase
… the Bacteroides conjugative transposon CTnDOT (IntDOT) might be a member of the tyrosine recombinase family because IntDOT has five of six highly conserved residues found in the catalytic domains of tyrosine recombinases. Yet, IntDOT catalyzes a reaction that appears to differ in some respects …
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Structure-function analysis of IntDOT, the CTnDOT integrase
IntDOT is an enzyme required for the recombination reactions that promote movement of CTnDOT that is an integrative conjugative element (ICE) found in Bacteroides spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require …
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Bacteroides Conjugative Transposons: Ecology and Mechanisms
… recombination site and integrase of CTnDOT (IntDOT) are sufficient and essential for integration in vivo. Even though the integrase of CTnDOT belongs to the lambda integrase family of recombinases, the mechanism of integration of CTnDOT is different from that of phage lambda, the model for …
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Role of the core in CTnDOT integration
IntDOT is the tyrosine recombinase encoded by the Bacteroides conjugative transposon CTnDOT. It catalyzes integration and excision into and out of the bacterial host chromosome. Although it is a member of the well studied tyrosine recombinase family, IntDOT is of particular interest because it can …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… for transfer are catalyzed by an integrase, IntDOT. IntDOT is a member of the tyrosine recombinase family. It was previously known that a host factor was required for integration and that Escherichia coli Integration Host Factor (IHF) could substitute for the host factor in an in vitro …
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DNA -Protein Interactions in the CTnDOT Excisive Intasome
… studies indicated that Exc might interact with IntDOT during excision, as determined by electrophoretic mobility shift assays. Further studies of Exc are warranted to answer the many questions that still remain about its function.
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Protein/DNA interactions during site-specific recombination
… and excision of CTnDOT is mediated by IntDOT, which has been identified as a member of the tyrosine recombinase family. Previous DNase I footprinting experiments demonstrated that IntDOT interacts with five arm-type sites, but exactly how the arm-type sites participate in the …
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Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT
… predict that a gene encoded downstream of intDOT, orf2, is the mob transcriptional repressor. Taken together, the work described in this dissertation has further shed light on the intricate regulation governing the conjugative transfer of CTnDOT.
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Excision of CTnDOT
Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-01-24T19:34:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Keeton_Carolyn.docx: 6779335 bytes, checksum: 32b917b01d2ce2946ce4c38114fe84a2 (MD5) Keeton_Carolyn.pdf: 4011034 bytes, …