Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Insertional mutagenesis"”.
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Insertional mutagenesis in zebrafish using a pseudotyped retroviral vector
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Use of a pseudotyped retoviral vector to accomplish insertional mutagenesis in zebrafish
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Random DNA integrations as an approach to insertional mutagenesis in the zebrafish (Brachydanio rerio)
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1994.
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Validation-based insertional mutagenesis (VBIM) technology identifies adenomatous polypossis coli (APC) like protein (ALP) as a novel negative regulator of NF-κB
… cancer therapy. Using a novel validation-based insertional mutagenesis (VBIM) strategy, our lab has identified the novel adenomatous polyposis coli (APC) like protein (ALP) gene as a negative regulator of NF-κB. Preliminary studies from our lab demonstrated that overexpression of ALP led to …
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Enzymes involved in utilization of pullulan by Bacteroides thetaiotaomicron
… for pullulan utilization, I used directed insertional mutagenesis to inactivate the B. thetaiotaomicron pullulanase gene.
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Studies of the structure, evolution, and TonB-dependence of the colicin I receptor of Escherichia coli
… by iron. When the gene was inactivated by insertional mutagenesis, Cir expression and function were unaltered. The gene encoding the 32 kD protein was sequenced. The predicted protein sequence was not significantly related to any of the protein sequences found in the GenBank protein …
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ΜΗΧΑΝΙΣΜΟΙ ΟΓΚΟΓΕΝΕΣΗΣ ΔΙΑ ΤΟΥ ΛΕΥΧΑΙΜΟΓΟΝΟΥ ΙΟΥ ΤΟΥ MOLONEY (MOMULV)
… AND LATE STAGES IS DUE TO PROVIRUS MEDIATES INSERTIONAL MUTAGENESIS. USING THE INTEGRATED PROVIRUS AS A MARKER WE HAVE IDENTIFIED AND CHARACTERIZED FIVE INDEPENDENT LOCI TOUR OF WHICH (MEVI-1,2,3,4) ARE INVOLVED IN TUMOR INDUCTION WHILE THE FIFTH ONE (TPL-1) IS INVOLVED IN TUMOR PROGRESSION.
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Screening for Mutants in the Output Pathway of the Circadian Clock in Chlamydomonas Reinhardtii
… by screening mutants, which were generated by insertional mutagenesis via glass bead transformation. The plasmid pSP124S containing the ble marker was used to introduce mutations. The CABII-1 gene has been reported to show a circadian rhythm in expression. The reporter gene ARS2 that was …
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DEVELOPING MOLECULAR TOOLS TO IMPROVE TERPENOIDS PRODUCTION IN RHODOSPORIDIUM TORULODIES
… sequence. Meanwhile, we used random T-DNA insertional mutagenesis to generate a mutant pool of Rhodosporidium toruloides and screened for mutants that were able to grow at high levels of monoterpene, menthol. Two such mutants, RtDPR1 and Rt0S08, were successfully identified, which helps …
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… lentivirus). Integration-based approaches risk insertional mutagenesis and oncogene activation, yielding unpredictable, irreversible consequences. Inspired by latent DNA viruses with genomes persisting as episomes, we developed an extrachromosomal genetic technology (EGT). Our EGT, featuring an …
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Targeting and Reprogramming Tumor-Associated Macrophages with Polymeric Nanoparticle-Mediated mRNA Delivery for Cancer Treatment
… costly, time-consuming, and carries risks like insertional mutagenesis, making it inaccessible for many families. One promising solution is in vivo reprogramming using messenger RNA (mRNA). mRNA can directly encode therapeutically proteins without genomic integration, avoiding the risk of …
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Exosporium Morphogenesis in Bacillus Cereus and Bacillus Anthracis
… in this sample. Inactivation of rbp by insertional mutagenesis resulted in spores devoid of the hair-like nap typical of the exosporium, which suggests that Rbp could be a new component of the exosporium nap or could have a role in its assembly. GerQ, a protein that is crosslinked by …
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Development of broadly applicable transgenic tools for the transposon mutagenesis of the red flour beetle, Tribolium castaneum
… systems, such as transposon-based insertional mutagenesis, can now be introduced to various insect species. To drive the transposase gene for transposable element remobilisation, suitable promoters are needed. The broadly conserved thermotolerance factor, Hsp70, is …
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Primary characterization of genes involved in the colony development of the insect pathogenic fungus Metarhizium anisopliae /
… conidiation. Following Agrobacterium tumefaciem insertional mutagenesis, phenotypic mutants were characterized using Y-shaped adaptor dependent extension PCR. Four of 1 8 colony development recombinants had T-DNA flanking sequences with high homology to genes encoding known signaling pathway …
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Hyperinvasiveness in the major food-borne pathogen Campylobacter jejuni
… generated in this strain by random transposon insertional mutagenesis were screened for their ability to invade human intestinal epithelial cells in an in vitro model and 174 mutants were selected for further studies. The motility of selected mutants was determined and 40 mutants that showed …
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Secondary Level Screening of Chlamydomonas Reinhardtii Mutants Defective in Circadian Gene Expression
… into the genome of this transformant via insertional mutagenesis to generate mutants defective in circadian CABII-1 expression. Potential mutants were selected based on aberrant single-point accumulative arylsulfatase activity. In this study, the arylsulfatase activity over the entire …
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Dissecting genetic determinants of transcription factor activity
… a set of 97 splenic tumors induced by retroviral insertional mutagenesis. We identify several instances of sequence-specific TFs whose activities are specifically affected by insertions mutations. Our results underscore the value of explicitly modeling TF activity and a strategy for finding …
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Morphological and Physiological Studies of the Carbon Concentrating Mechanism in Chlamydomonas reinhardtii
… on mutants with CCM defects generated by an insertional mutagenesis screen. Morphological and physiological characterisation of these mutants revealed differences in the pyrenoid morphology, the ability for RuBisCO to aggregate into the pyrenoid and the formation of thylakoidal tubule network …
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