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Showing 1 to 5 of 5 for “"Insertion efficiency"”.

  1. Development of biomimetic, hybrid protein-synthetic membranes: determining protein insertion efficiency, membrane material suitability and relevance of aquaporin Z

    … to understand the relationship between protein insertion and membrane permeability. This work introduces a method using fluorescence correlation spectroscopy to quantify the number of AqpZ embedded in the membrane. This work demonstrates that membrane permeability is a function of protein …

    uiuc Repository record for Development of biomimetic, hybrid protein-synthetic membranes: determining protein insertion efficiency, membrane material suitability and relevance of aquaporin Z (opens in a new tab)

  2. Targeting and Manipulating Endogenous Transcriptional Condensates

    … allow us to generate a quick response with high insertion efficiency. We adopt a proximity-based modification to biotinylate proteins inside of the condensates upon blue light exposure. This helps to determine the constituent of the condensates using mass spectrometry. Our approach opens the road …

    mit Repository record for Targeting and Manipulating Endogenous Transcriptional Condensates (opens in a new tab)

  3. DNA-Based Mimics of Membrane Proteins Lipid-DNA Interactions Determine Function

    … discussed surfactant’s influence on DNA membrane insertion efficiency, showing that aggregation of the nanostructures is one of the major factors determining their spontaneous membrane-spanning. While the understanding of phenomena in minimalistic systems is crucial for further development of …

    cambridge Repository record for DNA-Based Mimics of Membrane Proteins Lipid-DNA Interactions Determine Function (opens in a new tab)

  4. Biomimetic membranes as new materials for applications in envrionmental engineering and biology

    … diffraction. This indicates that the packing efficiency of these polymer-based systems is extremely high. Additionally, such crystals have the potential to allow for structural reconstruction of the incorporated aquaporins. This procedure can thus provide fundamental knowledge regarding the …

    uiuc Repository record for Biomimetic membranes as new materials for applications in envrionmental engineering and biology (opens in a new tab)

  5. Microfluidic Transport Studies on Lipid Vesicles

    … nanopore. We attribute this behaviour to the low insertion efficiency of the DNA nanopore and the temporal resolution of the assay, which might be too low when put in context with the timescale of passive proton permeation to show a potentially flux enhancing effect of the DNA nanostructure. …

    cambridge Repository record for Microfluidic Transport Studies on Lipid Vesicles (opens in a new tab)