Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 63 for “"Insect cells"”.
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Maintaining the balance: persistent baculovirus infection in insect cells
… californica nucleopolyhedrovirus (AcMNPV) are insect-specific viruses that usually kill the host. However, many species harbour persistent, non-lethal covert baculovirus infections although these have rarely been observed in vitro. Hence, little is known about the mechanisms for establishment …
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Isolation and characterisation of extracellular vesicles in baculovirus infection of insect cells
… multiple nucleopolyhedrovirus (AcMNPV) is an insect-specific virus. Its infection can be studied in Spodoptera frugiperda. It has a double-stranded DNA genome and a biphasic lifecycle, producing budded virus (BV) to infect neighbouring cells and occlusion derived virus to infect new insect …
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Induction and Inhibition of a Neuronal Phenotype in Spodoptera Frugiperda (Sf21) Insect Cells
Due to the increasing resistance demonstrated by insects to conventional insecticides, the need for compounds with novel modes of action is becoming more urgent. Also, the discovery and production of new insecticides is vital as regulations and restrictions on conventional insecticides become …
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Gene regulation in baculovirus-infected insect cells : application in the development of a budded virus free expression system
… LacI, was established. Infection of these cells with a recombinant virus expressing the “key” genes under the chiA/lacO promoter would allow LacI to bind lacO and block gene expression, resulting in a reduction in BV synthesis. Flow cytometry experiments showed that all single expression …
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Genetically engineering a new system for the expression of cytochrome P450 enzymes in insect cells using novel P450 reductases
… of ΔhRDM has allowed us to devise an insect cell expression system that genetically provides an improvement in the levels and activities of the drug metabolising cytochrome P450 enzymes.
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The use of insect cells to identify potent and selective inhibitors of the replication of the Dengue and Chikungunya viruses and unravel their molecular mechanism of action
… We established and characterized a new embryonic insect cell line from Culex quinquefasciatus mosquito. Also we established the flaviviruses and alphavirus replication, both in C6/36 and Lulo insect cell lines, as well as in Vero cell line. In addition we carried out a reference compound library …
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Insect expression systems : improving intracellular and heterologous gene expression
… foreign protein. Protein production in mammalian cells is a labour-intensive and expensive process and insect cells have been used as cheaper alternatives. Insect ES are based on two types of vector; viral- and plasmid-based. The baculovirus ES (BES) offers high-level transient production of …
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Functional Analysis of the Region Linking the N-Terminal Transmembrane Anchor and the Catalytic Domain of Cytochrome P450 2C2
… and Gly-rich region mutants expressed in COS-1 cells, bacteria, and insect cells. In COS-1 cells, mutations at Pro 30 and Pro33 in the Pro-rich region dramatically reduced activity, suggesting that a PXXP motif may be important for the formation or activity of a functional P450, and further that …
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Development of candidate Human papillomavirus vaccines
… was mutated to a threonine, were expressed in insect cells by recombinant baculovirus, and the resulting virus-like particles (VLPs) were tested with a panel of well-characterised monoclonal antibodies (Mabs).
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Investigation of the yeast oligosaccharyl transferase
… yeast Pichia pastoris and the lytic baculovirus/insect cell expression system. The results from P. pastoris suggested that the protein was not well behaved and expressed at low levels. Expression of the native membrane proteins in baculovirus-infected insect cells proved more successful as the …
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Biochemical and Molecular Approaches to Characterizing the Myrosinase Enzyme System in Horseradish (Armoracia Rusticana)
… expression of ArM1 in Spodoptera frugiperda insect cells resulted in an immune active myrosinase protein product and gave rise to a band of approximately 66 kDa in western blot analysis. ArM1 has the highest overall amino acid identity (72%) with Arabidopsis thaliana myrosinase TGG2. …
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Development of plant-produced Bluetongue virus vaccines
… (VLPs). BTV VLPs were successfully produced in insect cells by the co-expression of the four BTV capsid proteins (VP2, VP3, VP5 and VP7). Sheep vaccinated with insect cell-produced BTV VLPs were shown to be protected against challenge with wild type virus. However, the high costs associated with …
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Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
… expressed alone or as GFP fusions in E. coli and insect cells. The findings indicate that deletion of the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of membrane interaction such that …
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The molecular epidemiology of bluetongue virus in northern Colorado
… infection, using both mammalian cell lines and insect cells obtained from ABADRL, were performed to characterize a possible mechanism of persistence of the virus in insect cells. Viral nucleic acid was detected in presumably uninfected cell cultures, as well as in field-collected culicoid larvae …
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Analysis of the regulation of GIGANTEA transcript and protein accumulation in Arabidopsis and recombinant expression
… GI was expressed in baculovirus infected insect cells and generated GI protein that was more stable than that expressed in E. coli, but was still insoluble, possibly due to aggregation. Attempts were made to solubilise GI protein, and its localisation in insect cells was visualised using …
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Optimization of chimaeric HIV-1 virus-like particle (VLP) production and immunogenicity testing of VLPs in mice
… candidates (GagRT and GagTN) was optimized in insect cells, and their ability to enhance a murine immune response in a DNA prime-VLP boost vaccine strategy was evaluated.
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Isolation and characterization of the tubuliform spidroin 1 promoter from the black widow spider, Latrodectus Hesperus
… element was able to initiate transcription in insect cells when placed upstream the promoterless firefly luciferase reporter gene. Initiation of transcription was orientation dependent, as insertion of this upstream regulatory module in the reverse orientation led to inefficient transcriptional …
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Resin Detoxification in Dendroctonus ponderosae: Functional Characterizations of Cytochrome P450 Proteins
… do so, microsomal CYP6DH3 was expressed in Sf9 insect cells before being separated and incubated with various monoterpene substrates. GC screening revealed that no products could consistently be formed, which suggested an issue with the assay protocol. Various adjustments to the procedure have …
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Expression, purification and characterisation of recombinant chromatin assembly factor 1
… were expressed as complexes and individually in insect cells and Escherichia coli, optimised protocols are described for maximum protein recovery and purity. Constructs of p150 and p60 were also produced and used to analyse the binding regions and modes of both the p48 and p60 proteins to p150. …
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